Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31506
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23930.1 68416.m03006 expressed protein 33 0.17
At3g51010.1 68416.m05585 expressed protein 33 0.23
At5g52280.1 68418.m06488 protein transport protein-related low s... 32 0.31
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 31 0.53
At5g45520.1 68418.m05591 hypothetical protein 30 1.6
At5g25070.1 68418.m02971 expressed protein 30 1.6
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 30 1.6
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 2.2
At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to ... 29 2.8
At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 29 2.8
At5g03660.1 68418.m00325 expressed protein low similarity to out... 29 2.8
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 29 2.8
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 3.8
At1g01670.1 68414.m00085 U-box domain-containing protein 29 3.8
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 6.6
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 6.6
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 28 6.6
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 28 6.6
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 8.7
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 33.1 bits (72), Expect = 0.17
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 145 KPAPKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 264
K K + G IK +D++ + QLKE EWRK+R
Sbjct: 17 KETSKDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At3g51010.1 68416.m05585 expressed protein
Length = 188
Score = 32.7 bits (71), Expect = 0.23
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +1
Query: 415 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 594
I ++R + +KKRQA++Q K + +++K A ER +QLEEE
Sbjct: 125 IGQRRAFILSEKKKRQALVQEAKRKKRIKQ---VERKMAAVARDRAWAERLIELQQLEEE 181
Query: 595 KKISLS 612
KK S+S
Sbjct: 182 KKKSMS 187
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 32.3 bits (70), Expect = 0.31
Identities = 26/89 (29%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
Frame = +1
Query: 415 IEEKRQRLE-EAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLE- 588
++EK +RL E E K K N +Q K+ E + KEQ +
Sbjct: 583 LQEKCKRLSLEMESKLSEHENLTKKTLAEANNLRLQNKTLEEMQEKTHTEITQEKEQRKH 642
Query: 589 -EEKKISLSIRIKPLTIEGLSVDKLRQKA 672
EEK +LS++++ L E L + KLR ++
Sbjct: 643 VEEKNKALSMKVQMLESEVLKLTKLRDES 671
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 31.5 bits (68), Expect = 0.53
Identities = 22/89 (24%), Positives = 44/89 (49%), Gaps = 1/89 (1%)
Frame = +1
Query: 415 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQLEE 591
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+ +
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEKEVK 957
Query: 592 EKKISLSIRIKPLTIEGLSVDKLRQKAQE 678
EK I+ + E K R+K +E
Sbjct: 958 EKVTEKEIKERGGKDESRIQVKDRKKCEE 986
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.9 bits (64), Expect = 1.6
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +1
Query: 412 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENF-----GLSNAQLERNKTK 576
++E+ ++ E E++ + +++ KT P+ +K +N G SN + + +K K
Sbjct: 624 NLEDGKKHDEGKEERSLKSDEVVEEEKKTSPSEEATEKFQNKPGDQKGKSNVEGDGDKGK 683
Query: 577 EQLEEEKK 600
LEEEKK
Sbjct: 684 ADLEEEKK 691
>At5g25070.1 68418.m02971 expressed protein
Length = 736
Score = 29.9 bits (64), Expect = 1.6
Identities = 27/85 (31%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
Frame = +1
Query: 421 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE-EK 597
EKR EAEKK A + K+A + + KS N Q+E K +LE+ E
Sbjct: 565 EKRMPELEAEKKVAASTRNFKEAGR----IAAEAKSLNLEKDKTQMETGKANAELEKAEH 620
Query: 598 KISLSIRIKPLTIEGLSVDKLRQKA 672
+I +I+ + IE L + K ++ A
Sbjct: 621 EIEETIK-RLQEIEKLILSKEKELA 644
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 29.9 bits (64), Expect = 1.6
Identities = 26/74 (35%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +1
Query: 418 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK---EQLE 588
+EKR+R+EE E+K +KD N ++ + E SN +LE N K + +
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLN--LKTELEEARNSNVELELNNRKLSQDLVS 169
Query: 589 EEKKI-SLSIRIKP 627
E KI SLS KP
Sbjct: 170 AEAKISSLSSNDKP 183
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 154 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 252
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 304
Score = 29.1 bits (62), Expect = 2.8
Identities = 24/83 (28%), Positives = 40/83 (48%)
Frame = +1
Query: 415 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 594
I+++ +L++ KK QA + K +K I+K E + R K +LEE
Sbjct: 44 IDKQYDKLDKLLKKLQASHEESKSVTKAPAMKAIKKTMEKDVDEVGSIARF-IKGKLEEL 102
Query: 595 KKISLSIRIKPLTIEGLSVDKLR 663
+ +L+ R KP +G VD+ R
Sbjct: 103 DRENLANRQKPGCAKGSGVDRSR 125
>At5g05210.1 68418.m00555 nucleolar matrix protein-related contains
Pfam domain, PF04935: Surfeit locus protein 6
Length = 386
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/59 (20%), Positives = 33/59 (55%)
Frame = +1
Query: 427 RQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKI 603
+Q + + +K+++ + K+ + F ++K+ + G ++E+NK ++ + EKK+
Sbjct: 310 KQSIHKDKKRQEKNAEKWKERIEGQQKFKVEKQQKRSGNIADRIEQNKMRKIAKREKKL 368
>At5g03660.1 68418.m00325 expressed protein low similarity to outer
surface protein F [Borrelia burgdorferi] GI:466482;
contains Pfam profile PF04949: Family of unknown
function (DUF662)
Length = 173
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +1
Query: 478 MKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIRIK 624
+KD +F+ Q K E+ LS L K KE+ E+KK+ + R++
Sbjct: 18 LKDQLSESMSFSSQMKKEDDELSMKALSAFKAKEEEIEKKKMEIRERVQ 66
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 29.1 bits (62), Expect = 2.8
Identities = 23/71 (32%), Positives = 34/71 (47%)
Frame = +1
Query: 412 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 591
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
Query: 592 EKKISLSIRIK 624
+ + + K
Sbjct: 508 LSRRYVELEAK 518
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 412 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 561
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At1g01670.1 68414.m00085 U-box domain-containing protein
Length = 365
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 6/73 (8%)
Frame = +1
Query: 418 EEKRQRLEEAEKKRQ-----AMLQAMKDASKTGPNFT-IQKKSENFGLSNAQLERNKTKE 579
EE+R+RLE E KR+ M + ++A + T I E + E N+ K
Sbjct: 177 EEQRRRLEIEELKREKEQRDKMRRVREEALSSSSGVTKILYNEEVMRRREVEAELNRAKA 236
Query: 580 QLEEEKKISLSIR 618
++E+ K++ + ++
Sbjct: 237 EIEDMKRVQIELK 249
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 27.9 bits (59), Expect = 6.6
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = +1
Query: 439 EEAEKKRQAMLQAMKDASKTGPNFT--IQKKSENFGLSNAQLERNKTKEQLEEEKKISLS 612
EEA+K R+ L+ KDAS + + KK+E + +KE E+ ++ +
Sbjct: 710 EEAKKTREQALRKRKDASFKHVIISEKVDKKAEKLQTKSLPYPYT-SKEVFEQSMRMPIG 768
Query: 613 IRIKPLTIEGL 645
P TI G+
Sbjct: 769 PEFNPTTIVGV 779
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 185 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 307
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 27.9 bits (59), Expect = 6.6
Identities = 22/85 (25%), Positives = 38/85 (44%)
Frame = +1
Query: 415 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 594
+ E ++L + + ML+ + + S N QK+ E +N E KE++E
Sbjct: 684 LHELSEKLSFKTSQMERMLENLDEKSNEIDN---QKRHEEDVTANLNQEIKILKEEIENL 740
Query: 595 KKISLSIRIKPLTIEGLSVDKLRQK 669
KK S+ ++ E L VD + K
Sbjct: 741 KKNQDSLMLQAEQAENLRVDLEKTK 765
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 27.9 bits (59), Expect = 6.6
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Frame = +1
Query: 415 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKT---KEQL 585
+E+K + LEE +K + A+ A + G +IQ + +S + N++ +L
Sbjct: 155 LEQKLKSLEELSEKVSELKSALIVAEEEGKKSSIQMQEYQEKVSKLESSLNQSSARNSEL 214
Query: 586 EEEKKISL 609
EE+ +I+L
Sbjct: 215 EEDLRIAL 222
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +1
Query: 421 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQL---E 588
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 589 EEKK 600
EE+K
Sbjct: 504 EERK 507
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/61 (18%), Positives = 32/61 (52%)
Frame = +1
Query: 418 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 597
EE+++ EEA+++ + + ++A + ++K E + + K +E++E ++
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKR 549
Query: 598 K 600
+
Sbjct: 550 R 550
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.131 0.359
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,131,444
Number of Sequences: 28952
Number of extensions: 148115
Number of successful extensions: 654
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 653
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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