Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31503
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 2e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 57 3e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.19
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.8
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.1
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 27 4.1
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 7.2
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 9.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.9 bits (166), Expect = 2e-13
Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
Frame = +1
Query: 25 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 198
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 199 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 375
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 376 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 468
EVA++ ++ D DG+I Y F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 2e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +1
Query: 67 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 246
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 247 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 426
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 427 DDGMIPYAAFLKKVMA 474
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 57.2 bits (132), Expect = 3e-09
Identities = 37/146 (25%), Positives = 71/146 (48%)
Frame = +1
Query: 37 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 216
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 217 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 396
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 397 VTKDCMDPEDDDGMIPYAAFLKKVMA 474
+ ++ DP + G Y F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.1 bits (67), Expect = 0.19
Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
Frame = +1
Query: 67 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 243
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 244 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 423
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 424 DDDGMIPYAAFLKKVM 471
D DG I F+ +M
Sbjct: 158 DQDGEINEQEFIAIMM 173
Score = 29.5 bits (63), Expect = 0.58
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +1
Query: 235 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 408
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +1
Query: 34 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 150
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.8
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 627 APPEELSPPRALPAPVPQSRASVF*GPSHRT 535
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.1
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 25 MSDLSKNDVER-ASFAFSIYDFEGKGKID 108
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 26.6 bits (56), Expect = 4.1
Identities = 19/92 (20%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
Frame = +2
Query: 11 KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSRNSVVQ 187
++HT T A+ KG P++ KAK+ + ++ +S Q P+++S+ +
Sbjct: 43 RNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTKHSLDE 102
Query: 188 RRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 283
+ + SK S+ ++ + +IS
Sbjct: 103 NEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 7.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 361 LGEKLDDSEVAEVTKDCMDPED 426
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 9.5
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +2
Query: 44 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 217
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 218 KSSFPSTAKQRKTKT 262
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,987,122
Number of Sequences: 5004
Number of extensions: 54967
Number of successful extensions: 157
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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