BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31489 (550 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 33 0.028 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 29 0.34 SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 29 0.45 SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ... 28 1.0 SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc... 28 1.0 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 2.4 SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomy... 26 3.2 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 5.6 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 25 7.3 SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 9.7 SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 25 9.7 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 9.7 SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces po... 25 9.7 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 25 9.7 SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|c... 25 9.7 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 33.1 bits (72), Expect = 0.028 Identities = 20/61 (32%), Positives = 33/61 (54%) Frame = +1 Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543 EEKR+++EE++K + +LQA + K N + KKS + + KE+ + EK Sbjct: 138 EEKRRKIEESDKWHRVLLQA--EGKKLKDNEQLLKKSIRRKEKEKKKSSDAWKERKDNEK 195 Query: 544 K 546 K Sbjct: 196 K 196 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 29.5 bits (63), Expect = 0.34 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E + KR+A +A ++A + ++K++ NA+ E K K + E Sbjct: 636 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEENAKREAEEKVKRETE 694 Query: 535 EEKK 546 E K Sbjct: 695 ENAK 698 Score = 29.1 bits (62), Expect = 0.45 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E + KR+A +A ++A + ++K++ NA+ E K K + E Sbjct: 620 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEENAKREAEEKAKREAE 678 Query: 535 EEKK 546 E K Sbjct: 679 ENAK 682 Score = 27.5 bits (58), Expect = 1.4 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E + KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 572 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEEKAKREAEEKAKREAE 630 Query: 535 EEKK 546 E+ K Sbjct: 631 EKAK 634 Score = 27.5 bits (58), Expect = 1.4 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQK-KSENFGLSNAQLERNKTKEQLE 534 + EEK +R E + KR+A +A ++A + +K K E + + E N +E E Sbjct: 612 EAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 671 Query: 535 EEKK 546 + K+ Sbjct: 672 KAKR 675 Score = 26.6 bits (56), Expect = 2.4 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E + KR+A A ++A + ++K++ A+ E K K + E Sbjct: 580 EAEEKAKREAEEKAKREAEENAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 638 Query: 535 EEKK 546 E+ K Sbjct: 639 EKAK 642 Score = 26.6 bits (56), Expect = 2.4 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 588 EAEEKAKREAEENAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 646 Query: 535 EEKK 546 E+ K Sbjct: 647 EKAK 650 Score = 26.6 bits (56), Expect = 2.4 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534 + EEK +R E + KR+A +A ++A + ++K++ A+ E K K + E Sbjct: 604 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 662 Query: 535 EEKK 546 E K Sbjct: 663 ENAK 666 Score = 25.8 bits (54), Expect = 4.2 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +1 Query: 367 EKRQRLEEAEK-KRQAMLQAMKDASKTGPNLTIQK-KSENFGLSNAQLERNKTKEQLEEE 540 E++ RLE E KR+A QA ++A + +K K E + + E N +E E+ Sbjct: 550 EEKARLEAEENAKREAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKA 609 Query: 541 KK 546 K+ Sbjct: 610 KR 611 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 29.1 bits (62), Expect = 0.45 Identities = 16/67 (23%), Positives = 35/67 (52%), Gaps = 4/67 (5%) Frame = +1 Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKD----ASKTGPNLTIQKKSENFGLSNAQLERNKTKE 525 ++EEK LE A+ Q ++ ++KD +K + ++ G+S+A ++ K + Sbjct: 162 ELEEKINSLESAQSIEQEVISSLKDDKTVETKNDVPEVSRPSTDTIGVSSALSKKKKKRN 221 Query: 526 QLEEEKK 546 + ++KK Sbjct: 222 RKNQKKK 228 >SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 27.9 bits (59), Expect = 1.0 Identities = 17/54 (31%), Positives = 31/54 (57%) Frame = +1 Query: 382 LEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543 +EE E+ R+ + A D + G + +S+ G + +++ KTK+QLEEE+ Sbjct: 197 VEEQERLRKETIAAFHDVN--GNKDAVSNESDEDG--DFLVKKEKTKKQLEEEE 246 >SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 776 Score = 27.9 bits (59), Expect = 1.0 Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%) Frame = +1 Query: 370 KRQRLEEAEKKRQAMLQAMKDASKTGPNLT---IQKKSENFGLSNAQLERNKTKEQLEEE 540 K + +++R A +QA+ D S T + + +SEN GL N ++ +QLE+E Sbjct: 232 KDHEILRLKEERTAAMQAIDDISGTIETIKSDCYKVESENKGLINEVMDMRNFVQQLEQE 291 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 26.6 bits (56), Expect = 2.4 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -2 Query: 210 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG 94 P+FA + L LF+Q+ P + + FW S+ L+ +G Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAG 831 >SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 274 Score = 26.2 bits (55), Expect = 3.2 Identities = 16/64 (25%), Positives = 35/64 (54%), Gaps = 2/64 (3%) Frame = +1 Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTK--EQLEE 537 EEK +++EE E + + ++D K+ +++K S A+ +R+ + E LE+ Sbjct: 132 EEKDRKIEEGELTSEEEKEPIQDLRKSHKRKSVEKSSVPNKKHKAERKRSPSPKIEILED 191 Query: 538 EKKI 549 +++I Sbjct: 192 DEEI 195 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 5.6 Identities = 8/24 (33%), Positives = 14/24 (58%) Frame = -3 Query: 476 SLFFWMVKLGPVLLASFMAWSIAW 405 SL+FW L P +F++W + + Sbjct: 111 SLYFWTAYLSPPKYRAFLSWFLGY 134 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.0 bits (52), Expect = 7.3 Identities = 11/20 (55%), Positives = 16/20 (80%) Frame = +1 Query: 127 EFIKRQDQKRSDLDEQLKEY 186 E +KR+DQK+ D+ E L+EY Sbjct: 272 ELLKRRDQKQQDV-EALQEY 290 >SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces pombe|chr 2|||Manual Length = 857 Score = 24.6 bits (51), Expect = 9.7 Identities = 13/31 (41%), Positives = 14/31 (45%) Frame = +2 Query: 182 NTSTNGANSGPRRRMSSNALKRSRPSARFLV 274 N A P SSN+ K S PS FLV Sbjct: 793 NIPLGHALGNPESNNSSNSFKPSHPSQSFLV 823 >SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 24.6 bits (51), Expect = 9.7 Identities = 12/40 (30%), Positives = 22/40 (55%) Frame = -2 Query: 210 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSGC 91 PL + +D + ++F+Q++ VL + SL + SGC Sbjct: 281 PLLSLSIDFYFRVFVQIKAKPVLVKNLQSQSLLIYHCSGC 320 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 24.6 bits (51), Expect = 9.7 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = +1 Query: 367 EKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLS 492 +++ L K A+LQ M G LT+Q+ ++NFG S Sbjct: 1098 DRKSSLSSVSDKDAAVLQRM------GAQLTLQQMAQNFGSS 1133 >SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces pombe|chr 3|||Manual Length = 218 Score = 24.6 bits (51), Expect = 9.7 Identities = 16/58 (27%), Positives = 27/58 (46%) Frame = +1 Query: 361 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLE 534 + EK+ + K A ++A + ASK+G T S+N L + +R + K E Sbjct: 116 LHEKKVIDDNQYKDAMATVEAHEQASKSGDTSTSGAISKNDILKRIEEDRERHKRMRE 173 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 24.6 bits (51), Expect = 9.7 Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 3/62 (4%) Frame = +1 Query: 367 EKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLER---NKTKEQLEE 537 E RQ+ EE ++++QAM +A + A+ T ++ SE LS + +R K K++LE Sbjct: 55 ELRQKYEEEQRRQQAM-EAKRIAAST------RQTSERPPLSAEEAKRIREVKEKDRLES 107 Query: 538 EK 543 +K Sbjct: 108 KK 109 >SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 24.6 bits (51), Expect = 9.7 Identities = 15/52 (28%), Positives = 30/52 (57%) Frame = +1 Query: 391 AEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEKK 546 A + RQ + + ++++ KT L K E+ + + E N+TK++++ EKK Sbjct: 174 ARRTRQRLAEHLENSKKT--ILQHDNKEEDKEIHS---EENETKDEIKSEKK 220 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.309 0.125 0.338 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,324,763 Number of Sequences: 5004 Number of extensions: 17882 Number of successful extensions: 90 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.7 bits)
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