SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31489
         (550 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo...    33   0.028
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo...    29   0.34 
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch...    29   0.45 
SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ...    28   1.0  
SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc...    28   1.0  
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    27   2.4  
SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomy...    26   3.2  
SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy...    25   5.6  
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom...    25   7.3  
SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces...    25   9.7  
SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera...    25   9.7  
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    25   9.7  
SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces po...    25   9.7  
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac...    25   9.7  
SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|c...    25   9.7  

>SPBC947.07 |||ribosome biogenesis protein
           Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 233

 Score = 33.1 bits (72), Expect = 0.028
 Identities = 20/61 (32%), Positives = 33/61 (54%)
 Frame = +1

Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543
           EEKR+++EE++K  + +LQA  +  K   N  + KKS        +   +  KE+ + EK
Sbjct: 138 EEKRRKIEESDKWHRVLLQA--EGKKLKDNEQLLKKSIRRKEKEKKKSSDAWKERKDNEK 195

Query: 544 K 546
           K
Sbjct: 196 K 196


>SPAC17C9.03 |tif471||translation initiation factor eIF4G
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1403

 Score = 29.5 bits (63), Expect = 0.34
 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E + KR+A  +A ++A +       ++K++     NA+ E   K K + E
Sbjct: 636 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEENAKREAEEKVKRETE 694

Query: 535 EEKK 546
           E  K
Sbjct: 695 ENAK 698



 Score = 29.1 bits (62), Expect = 0.45
 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E + KR+A  +A ++A +       ++K++     NA+ E   K K + E
Sbjct: 620 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEENAKREAEEKAKREAE 678

Query: 535 EEKK 546
           E  K
Sbjct: 679 ENAK 682



 Score = 27.5 bits (58), Expect = 1.4
 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E + KR+A  +A ++A +       ++K++      A+ E   K K + E
Sbjct: 572 EAEEKAKREAEEKAKREAEEKAKREAEENAKR-EAEEKAKREAEEKAKREAEEKAKREAE 630

Query: 535 EEKK 546
           E+ K
Sbjct: 631 EKAK 634



 Score = 27.5 bits (58), Expect = 1.4
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQK-KSENFGLSNAQLERNKTKEQLE 534
           + EEK +R  E + KR+A  +A ++A +       +K K E    +  + E N  +E  E
Sbjct: 612 EAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 671

Query: 535 EEKK 546
           + K+
Sbjct: 672 KAKR 675



 Score = 26.6 bits (56), Expect = 2.4
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E + KR+A   A ++A +       ++K++      A+ E   K K + E
Sbjct: 580 EAEEKAKREAEEKAKREAEENAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 638

Query: 535 EEKK 546
           E+ K
Sbjct: 639 EKAK 642



 Score = 26.6 bits (56), Expect = 2.4
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E   KR+A  +A ++A +       ++K++      A+ E   K K + E
Sbjct: 588 EAEEKAKREAEENAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 646

Query: 535 EEKK 546
           E+ K
Sbjct: 647 EKAK 650



 Score = 26.6 bits (56), Expect = 2.4
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLE-RNKTKEQLE 534
           + EEK +R  E + KR+A  +A ++A +       ++K++      A+ E   K K + E
Sbjct: 604 EAEEKAKREAEEKAKREAEEKAKREAEEKAKR-EAEEKAKREAEEKAKREAEEKAKREAE 662

Query: 535 EEKK 546
           E  K
Sbjct: 663 ENAK 666



 Score = 25.8 bits (54), Expect = 4.2
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = +1

Query: 367 EKRQRLEEAEK-KRQAMLQAMKDASKTGPNLTIQK-KSENFGLSNAQLERNKTKEQLEEE 540
           E++ RLE  E  KR+A  QA ++A +       +K K E    +  + E N  +E  E+ 
Sbjct: 550 EEKARLEAEENAKREAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREAEEKA 609

Query: 541 KK 546
           K+
Sbjct: 610 KR 611


>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 750

 Score = 29.1 bits (62), Expect = 0.45
 Identities = 16/67 (23%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
 Frame = +1

Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKD----ASKTGPNLTIQKKSENFGLSNAQLERNKTKE 525
           ++EEK   LE A+   Q ++ ++KD     +K       +  ++  G+S+A  ++ K + 
Sbjct: 162 ELEEKINSLESAQSIEQEVISSLKDDKTVETKNDVPEVSRPSTDTIGVSSALSKKKKKRN 221

Query: 526 QLEEEKK 546
           +  ++KK
Sbjct: 222 RKNQKKK 228


>SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 598

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 17/54 (31%), Positives = 31/54 (57%)
 Frame = +1

Query: 382 LEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543
           +EE E+ R+  + A  D +  G    +  +S+  G  +  +++ KTK+QLEEE+
Sbjct: 197 VEEQERLRKETIAAFHDVN--GNKDAVSNESDEDG--DFLVKKEKTKKQLEEEE 246


>SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence
           orphan|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 776

 Score = 27.9 bits (59), Expect = 1.0
 Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
 Frame = +1

Query: 370 KRQRLEEAEKKRQAMLQAMKDASKTGPNLT---IQKKSENFGLSNAQLERNKTKEQLEEE 540
           K   +   +++R A +QA+ D S T   +     + +SEN GL N  ++     +QLE+E
Sbjct: 232 KDHEILRLKEERTAAMQAIDDISGTIETIKSDCYKVESENKGLINEVMDMRNFVQQLEQE 291


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 26.6 bits (56), Expect = 2.4
 Identities = 13/39 (33%), Positives = 22/39 (56%)
 Frame = -2

Query: 210 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG 94
           P+FA  +   L LF+Q+ P + +    FW S+ L+  +G
Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAG 831


>SPBC2A9.07c |||zf-PARP-type zinc finger protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 274

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 16/64 (25%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
 Frame = +1

Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTK--EQLEE 537
           EEK +++EE E   +   + ++D  K+    +++K S       A+ +R+ +   E LE+
Sbjct: 132 EEKDRKIEEGELTSEEEKEPIQDLRKSHKRKSVEKSSVPNKKHKAERKRSPSPKIEILED 191

Query: 538 EKKI 549
           +++I
Sbjct: 192 DEEI 195


>SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 543

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 8/24 (33%), Positives = 14/24 (58%)
 Frame = -3

Query: 476 SLFFWMVKLGPVLLASFMAWSIAW 405
           SL+FW   L P    +F++W + +
Sbjct: 111 SLYFWTAYLSPPKYRAFLSWFLGY 134


>SPAC6F6.12 |||autophagy associated protein
           Atg24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 401

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 11/20 (55%), Positives = 16/20 (80%)
 Frame = +1

Query: 127 EFIKRQDQKRSDLDEQLKEY 186
           E +KR+DQK+ D+ E L+EY
Sbjct: 272 ELLKRRDQKQQDV-EALQEY 290


>SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 857

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 13/31 (41%), Positives = 14/31 (45%)
 Frame = +2

Query: 182 NTSTNGANSGPRRRMSSNALKRSRPSARFLV 274
           N     A   P    SSN+ K S PS  FLV
Sbjct: 793 NIPLGHALGNPESNNSSNSFKPSHPSQSFLV 823


>SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA
           methyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 548

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 12/40 (30%), Positives = 22/40 (55%)
 Frame = -2

Query: 210 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSGC 91
           PL +  +D + ++F+Q++   VL  +    SL +   SGC
Sbjct: 281 PLLSLSIDFYFRVFVQIKAKPVLVKNLQSQSLLIYHCSGC 320


>SPBC1826.01c |mot1||TATA-binding protein associated factor
            Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = +1

Query: 367  EKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLS 492
            +++  L     K  A+LQ M      G  LT+Q+ ++NFG S
Sbjct: 1098 DRKSSLSSVSDKDAAVLQRM------GAQLTLQQMAQNFGSS 1133


>SPCC4B3.08 |||C-terminal domain kinase I |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 218

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 16/58 (27%), Positives = 27/58 (46%)
 Frame = +1

Query: 361 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLE 534
           + EK+   +   K   A ++A + ASK+G   T    S+N  L   + +R + K   E
Sbjct: 116 LHEKKVIDDNQYKDAMATVEAHEQASKSGDTSTSGAISKNDILKRIEEDRERHKRMRE 173


>SPCC1393.02c |||non-specific DNA binding protein Spt2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 406

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 3/62 (4%)
 Frame = +1

Query: 367 EKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLER---NKTKEQLEE 537
           E RQ+ EE ++++QAM +A + A+ T      ++ SE   LS  + +R    K K++LE 
Sbjct: 55  ELRQKYEEEQRRQQAM-EAKRIAAST------RQTSERPPLSAEEAKRIREVKEKDRLES 107

Query: 538 EK 543
           +K
Sbjct: 108 KK 109


>SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 432

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 15/52 (28%), Positives = 30/52 (57%)
 Frame = +1

Query: 391 AEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEKK 546
           A + RQ + + ++++ KT   L    K E+  + +   E N+TK++++ EKK
Sbjct: 174 ARRTRQRLAEHLENSKKT--ILQHDNKEEDKEIHS---EENETKDEIKSEKK 220


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.309    0.125    0.338 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,324,763
Number of Sequences: 5004
Number of extensions: 17882
Number of successful extensions: 90
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.7 bits)

- SilkBase 1999-2023 -