Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31489
(550 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23930.1 68416.m03006 expressed protein 32 0.29
At5g45520.1 68418.m05591 hypothetical protein 30 1.2
At3g51010.1 68416.m05585 expressed protein 30 1.2
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 30 1.2
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 1.5
At2g14140.1 68415.m01575 hypothetical protein similar to At2g049... 29 2.0
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 29 2.7
At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to... 29 2.7
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 28 3.6
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 4.7
At5g24290.2 68418.m02858 integral membrane family protein contai... 27 6.2
At5g24290.1 68418.m02857 integral membrane family protein contai... 27 6.2
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 6.2
At5g23575.1 68418.m02766 transmembrane protein, putative similar... 27 8.3
At1g01670.1 68414.m00085 U-box domain-containing protein 27 8.3
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 31.9 bits (69), Expect = 0.29
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 103 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 210
K + G IK +D++ + QLKE EWRK+R
Sbjct: 21 KDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.9 bits (64), Expect = 1.2
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +1
Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENF-----GLSNAQLERNKTK 522
++E+ ++ E E++ + +++ KT P+ +K +N G SN + + +K K
Sbjct: 624 NLEDGKKHDEGKEERSLKSDEVVEEEKKTSPSEEATEKFQNKPGDQKGKSNVEGDGDKGK 683
Query: 523 EQLEEEKK 546
LEEEKK
Sbjct: 684 ADLEEEKK 691
>At3g51010.1 68416.m05585 expressed protein
Length = 188
Score = 29.9 bits (64), Expect = 1.2
Identities = 20/62 (32%), Positives = 31/62 (50%)
Frame = +1
Query: 361 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEE 540
I ++R + +KKRQA++Q K + +++K A ER +QLEEE
Sbjct: 125 IGQRRAFILSEKKKRQALVQEAKRKKRIKQ---VERKMAAVARDRAWAERLIELQQLEEE 181
Query: 541 KK 546
KK
Sbjct: 182 KK 183
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 29.9 bits (64), Expect = 1.2
Identities = 16/62 (25%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Frame = +1
Query: 361 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNA-QLERNKTKEQLEE 537
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+ +
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEKEVK 957
Query: 538 EK 543
EK
Sbjct: 958 EK 959
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 1.5
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 100 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 198
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At2g14140.1 68415.m01575 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At3g30450, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 847
Score = 29.1 bits (62), Expect = 2.0
Identities = 18/59 (30%), Positives = 31/59 (52%)
Frame = +1
Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEE 540
EE++Q E E++ + + KD T N+ +K EN + ++ER + KE+ EE
Sbjct: 559 EEEKQEEEGKEEEEEKICVEYKDHHSTC-NVEETEKQENPKQGDEEMEREEGKEEKVEE 616
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 28.7 bits (61), Expect = 2.7
Identities = 22/60 (36%), Positives = 30/60 (50%)
Frame = +1
Query: 358 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEE 537
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
>At1g08730.1 68414.m00969 myosin heavy chain (PCR43) identical to
myosin heavy chain PCR43 (PIR:T00727) [Arabidopsis
thaliana]; similar to ESTs gb|R30087 and gb|AA394762
Length = 1538
Score = 28.7 bits (61), Expect = 2.7
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +1
Query: 361 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKK 471
+E+K+++LEE EKK Q + +++ + NL + K
Sbjct: 1018 LEDKKKKLEETEKKGQQLQESLTRMEEKCSNLESENK 1054
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 28.3 bits (60), Expect = 3.6
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = +1
Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTK 522
+EKR+R+EE E+K +KD NL + + E SN +LE N K
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLNL--KTELEEARNSNVELELNNRK 162
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 4.7
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 131 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 253
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At5g24290.2 68418.m02858 integral membrane family protein contains
Pfam domain PF01988: Integral membrane protein
Length = 534
Score = 27.5 bits (58), Expect = 6.2
Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 6/68 (8%)
Frame = +1
Query: 358 DIEEKRQR------LEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKT 519
++E KRQR +EE E+ + L ++ S L Q EN+ L N E+N+
Sbjct: 116 NVENKRQRNGEDCEIEEEEENNERSLSDSEEKSNLEKLLGTQ---ENYELGNEDEEKNER 172
Query: 520 KEQLEEEK 543
EEK
Sbjct: 173 SSSDSEEK 180
>At5g24290.1 68418.m02857 integral membrane family protein contains
Pfam domain PF01988: Integral membrane protein
Length = 550
Score = 27.5 bits (58), Expect = 6.2
Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 6/68 (8%)
Frame = +1
Query: 358 DIEEKRQR------LEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKT 519
++E KRQR +EE E+ + L ++ S L Q EN+ L N E+N+
Sbjct: 132 NVENKRQRNGEDCEIEEEEENNERSLSDSEEKSNLEKLLGTQ---ENYELGNEDEEKNER 188
Query: 520 KEQLEEEK 543
EEK
Sbjct: 189 SSSDSEEK 196
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.5 bits (58), Expect = 6.2
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +1
Query: 367 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQL---E 534
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 535 EEKK 546
EE+K
Sbjct: 504 EERK 507
Score = 27.5 bits (58), Expect = 6.2
Identities = 11/61 (18%), Positives = 32/61 (52%)
Frame = +1
Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543
EE+++ EEA+++ + + ++A + ++K E + + K +E++E ++
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKR 549
Query: 544 K 546
+
Sbjct: 550 R 550
Score = 27.1 bits (57), Expect = 8.3
Identities = 15/61 (24%), Positives = 30/61 (49%)
Frame = +1
Query: 364 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNLTIQKKSENFGLSNAQLERNKTKEQLEEEK 543
EE+++R EEAE+ R+ + K+ +++ E + + E + K + EE +
Sbjct: 504 EERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEEAR 563
Query: 544 K 546
K
Sbjct: 564 K 564
>At5g23575.1 68418.m02766 transmembrane protein, putative similar to
cleft lip and palate transmembrane protein 1 [Homo
sapiens] GI:4039014; contains Pfam profile PF05602:
Cleft lip and palate transmembrane protein 1 (CLPTM1)
Length = 593
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 97 APKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWR 201
+PK E +PE K D+K +E E+I+ W+
Sbjct: 182 SPKDSDESEPEVEKVGDKKSDPKEEVPVEWISLWK 216
>At1g01670.1 68414.m00085 U-box domain-containing protein
Length = 365
Score = 27.1 bits (57), Expect = 8.3
Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 6/68 (8%)
Frame = +1
Query: 364 EEKRQRLEEAEKKRQ-----AMLQAMKDASKTGPNLT-IQKKSENFGLSNAQLERNKTKE 525
EE+R+RLE E KR+ M + ++A + +T I E + E N+ K
Sbjct: 177 EEQRRRLEIEELKREKEQRDKMRRVREEALSSSSGVTKILYNEEVMRRREVEAELNRAKA 236
Query: 526 QLEEEKKI 549
++E+ K++
Sbjct: 237 EIEDMKRV 244
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.309 0.125 0.338
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,242,016
Number of Sequences: 28952
Number of extensions: 106679
Number of successful extensions: 350
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 333
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 350
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.7 bits)
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