BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31476
(617 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25 |Sch... 32 0.076
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 27 2.2
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 27 2.9
SPAC21E11.05c |cyp8||cyclophilin family peptidyl-prolyl cis-tran... 27 2.9
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 26 3.8
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 26 5.0
SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|c... 25 8.8
>SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 31.9 bits (69), Expect = 0.076
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 4/45 (8%)
Frame = +3
Query: 120 GETCGSNGLPLTPSSISILGRAQR--LLS--IEHPNLCTYLDIIR 242
GE +P TP++ S R QR LL + HPN+C +D IR
Sbjct: 76 GEKAALKMIPFTPNNTSQTVRVQREALLGRLLRHPNICRVIDCIR 120
>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
Sap49|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -2
Query: 184 ARPKIDIELGVRGSPLLPQVSPG*LLAKNVHAA 86
A P +++ +G G+PL+P PG ++A AA
Sbjct: 273 AMPMMNVPMGPGGAPLVPPPPPGMVMASPSPAA 305
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 26.6 bits (56), Expect = 2.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -2
Query: 220 HKFGCSMDSSLCARPKIDIELGVRGSPLLPQVS 122
H SMDS+ P+ D+ VR S +PQ S
Sbjct: 458 HYIDSSMDSNRSIEPEPDVISTVRNSSTIPQAS 490
>SPAC21E11.05c |cyp8||cyclophilin family peptidyl-prolyl cis-trans
isomerase Cyp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 516
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -1
Query: 167 YRTGSKGQSITSTSFTRVTLSEK 99
+ TG S+TSTSFT VT +E+
Sbjct: 235 HTTGYAAASLTSTSFTPVTKNER 257
>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 589
Score = 26.2 bits (55), Expect = 3.8
Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +3
Query: 207 HPNLCTYLD--IIRGKHGKVVVSQNKI*LKS*TIRNRKIT*THFEELY*QNCSRLHFL 374
HPN+ TYLD ++R +H VV+ + I K+T + + C + L
Sbjct: 368 HPNIVTYLDSFLVRERHLWVVMEYMNAGSLTDIIEKSKLTEAQIARICLETCKGIQHL 425
>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1337
Score = 25.8 bits (54), Expect = 5.0
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = -3
Query: 417 SYFNLQNLAQKYEKIKNA-IWS--SSVNIIPQNV 325
S+F+ NL +KY +KN W+ S+ IP+N+
Sbjct: 172 SFFSKSNLKRKYRYLKNPDAWNLPPSLQFIPKNL 205
>SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|chr
2|||Manual
Length = 812
Score = 25.0 bits (52), Expect = 8.8
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +2
Query: 110 ESPW*NLWK*WTAPYSQFYINL 175
+S W LW+ W YS F +++
Sbjct: 82 QSRWSKLWRIWNVGYSWFILSI 103
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,510,424
Number of Sequences: 5004
Number of extensions: 48481
Number of successful extensions: 109
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -