Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31466
(669 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 355 3e-99
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 206 2e-54
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 188 5e-49
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 145 4e-36
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 107 1e-24
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 107 1e-24
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 95 6e-21
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 53 3e-08
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.002
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.15
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.26
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.3
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 9.9
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 9.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 355 bits (873), Expect = 3e-99
Identities = 159/175 (90%), Positives = 171/175 (97%)
Frame = +1
Query: 58 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 237
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 238 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 417
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 418 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 582
I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIM 176
Score = 56.4 bits (130), Expect = 3e-09
Identities = 26/33 (78%), Positives = 28/33 (84%)
Frame = +2
Query: 569 PTPSFRLDLAGRDLTDYLMKILTERGYSFTTTA 667
P RLDLAGRDLTDYLMKIL ERGY+F+TTA
Sbjct: 172 PHAIMRLDLAGRDLTDYLMKILMERGYTFSTTA 204
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 206 bits (504), Expect = 2e-54
Identities = 94/179 (52%), Positives = 129/179 (72%), Gaps = 1/179 (0%)
Frame = +1
Query: 64 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 243
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 244 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 420
+L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ +
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127
Query: 421 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILPSGLG 597
+ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI +G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIG 186
Score = 26.6 bits (56), Expect = 3.2
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +2
Query: 590 DLAGRDLTDYLMKILTERGYSFTTTA 667
++ GRD+TDYL L + G+ ++A
Sbjct: 184 EIGGRDVTDYLQLQLRKSGHELVSSA 209
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 188 bits (459), Expect = 5e-49
Identities = 87/172 (50%), Positives = 123/172 (71%), Gaps = 2/172 (1%)
Frame = +1
Query: 70 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 243
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 244 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 423
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123
Query: 424 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI 579
FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H +
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLV 175
Score = 38.7 bits (86), Expect = 7e-04
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +2
Query: 584 RLDLAGRDLTDYLMKILTERGYSFTTTA 667
RLD+AGRD T YL+ +L +GY+F TA
Sbjct: 177 RLDVAGRDATRYLISLLLRKGYAFNRTA 204
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 145 bits (352), Expect = 4e-36
Identities = 82/200 (41%), Positives = 113/200 (56%), Gaps = 32/200 (16%)
Frame = +1
Query: 76 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 204
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 205 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 363
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 364 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 519
LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186
Query: 520 GDGVSHTVPIYEGYALPHAI 579
GDGV+H +P+ EGY + +I
Sbjct: 187 GDGVTHIIPVAEGYVIGSSI 206
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 107 bits (257), Expect = 1e-24
Identities = 60/187 (32%), Positives = 108/187 (57%), Gaps = 3/187 (1%)
Frame = +1
Query: 7 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 186
E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R +
Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61
Query: 187 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 363
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+
Sbjct: 62 --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118
Query: 364 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 537
+TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH
Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178
Query: 538 TVPIYEG 558
+P+ G
Sbjct: 179 VIPVLNG 185
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 107 bits (257), Expect = 1e-24
Identities = 59/169 (34%), Positives = 91/169 (53%)
Frame = +1
Query: 61 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 240
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 241 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 420
G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 421 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 567
MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 95.5 bits (227), Expect = 6e-21
Identities = 50/180 (27%), Positives = 95/180 (52%), Gaps = 2/180 (1%)
Frame = +1
Query: 61 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 240
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 241 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 414
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 415 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILPSGL 594
+ FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 53.2 bits (122), Expect = 3e-08
Identities = 31/103 (30%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +1
Query: 262 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 429
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 430 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 558
P +A + ++ LYA G G+V+D G + PI +G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 37.5 bits (83), Expect = 0.002
Identities = 42/174 (24%), Positives = 70/174 (40%), Gaps = 6/174 (3%)
Frame = +1
Query: 76 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 255
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 256 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 423
++ H G + +W +W N + P ++ +LLT+ + Q++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 424 FETFNSPAMYVAIQAVLSLYASGRTTGIVLD--SGDGVSHTVPIYEGYALPHAI 579
FE F A A L + G T D +G + I GY+ H I
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHII 171
Score = 25.4 bits (53), Expect = 7.5
Identities = 9/23 (39%), Positives = 18/23 (78%)
Frame = +2
Query: 584 RLDLAGRDLTDYLMKILTERGYS 652
R+D+ G+ LT+YL ++++ R Y+
Sbjct: 185 RVDVGGKLLTNYLKEVISYRKYN 207
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.15
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +1
Query: 121 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 264
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.26
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 322 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 465
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.3
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 364 LLTEAPLNPKANREKMTQIMFETFN 438
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 791
Score = 25.0 bits (52), Expect = 9.9
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = -2
Query: 317 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 171
WC+ S + SQ+ + +G F PL L WA + + P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 181 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 267
+M+G +Y+G AQ KR +LT + I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,591,380
Number of Sequences: 5004
Number of extensions: 52861
Number of successful extensions: 201
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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