BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31465 (535 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 27 1.3 SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 26 4.1 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 5.4 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 7.1 SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe... 25 9.4 >SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 396 Score = 27.5 bits (58), Expect = 1.3 Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%) Frame = +1 Query: 244 ALEVRGQYSYVDLDGKVH---ETTYTADENGFHPSGADIPQLPQV 369 ++E G Y ++ L+G VH T T + H S A + LPQV Sbjct: 199 SIEGEGAYGHLMLEGGVHRVQRTPATETKGRVHTSTASVIVLPQV 243 >SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 25.8 bits (54), Expect = 4.1 Identities = 12/30 (40%), Positives = 13/30 (43%) Frame = -1 Query: 310 CTSSRGPFHRGRRTSTVREPQEPHFRYRRS 221 C +H GR S V P PHF R S Sbjct: 81 CPPQFEEYHHGRSHSLVSPPPSPHFLKRIS 110 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 5.4 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = -2 Query: 273 VRVLSANLKSLIFGTDVLQYSRFLGCFAIRSFKAKAVTLNADIV 142 ++++ + LK LI + L SRF A +F K + N D+V Sbjct: 227 LQIIKSKLKELIGTSIFLAESRFRKLEANNAFHRKPIIKNRDVV 270 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 25.0 bits (52), Expect = 7.1 Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 3/46 (6%) Frame = -1 Query: 346 LLRRDGNHFRQRCTSSRGPFHRGRRTSTVREPQEPHFR---YRRSS 218 L DGNHFR + S P R+ S + E + + YRRSS Sbjct: 60 LNENDGNHFRPASSLSFSPSSLSRKDSGPGDGLEVNKKNNFYRRSS 105 >SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 24.6 bits (51), Expect = 9.4 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = -2 Query: 483 FYSLPYRVFSVQDPNVADDRQHFVRSL 403 +YS P +F Q P +A+ Q F+R L Sbjct: 108 WYSKPILIFLHQTPELAEASQKFLRYL 134 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,843,525 Number of Sequences: 5004 Number of extensions: 34357 Number of successful extensions: 89 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 89 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 220420454 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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