BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31465
(535 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 27 1.3
SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 26 4.1
SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 5.4
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 7.1
SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe... 25 9.4
>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 396
Score = 27.5 bits (58), Expect = 1.3
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +1
Query: 244 ALEVRGQYSYVDLDGKVH---ETTYTADENGFHPSGADIPQLPQV 369
++E G Y ++ L+G VH T T + H S A + LPQV
Sbjct: 199 SIEGEGAYGHLMLEGGVHRVQRTPATETKGRVHTSTASVIVLPQV 243
>SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 25.8 bits (54), Expect = 4.1
Identities = 12/30 (40%), Positives = 13/30 (43%)
Frame = -1
Query: 310 CTSSRGPFHRGRRTSTVREPQEPHFRYRRS 221
C +H GR S V P PHF R S
Sbjct: 81 CPPQFEEYHHGRSHSLVSPPPSPHFLKRIS 110
>SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor
complex subunit Rrn5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 556
Score = 25.4 bits (53), Expect = 5.4
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = -2
Query: 273 VRVLSANLKSLIFGTDVLQYSRFLGCFAIRSFKAKAVTLNADIV 142
++++ + LK LI + L SRF A +F K + N D+V
Sbjct: 227 LQIIKSKLKELIGTSIFLAESRFRKLEANNAFHRKPIIKNRDVV 270
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 25.0 bits (52), Expect = 7.1
Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Frame = -1
Query: 346 LLRRDGNHFRQRCTSSRGPFHRGRRTSTVREPQEPHFR---YRRSS 218
L DGNHFR + S P R+ S + E + + YRRSS
Sbjct: 60 LNENDGNHFRPASSLSFSPSSLSRKDSGPGDGLEVNKKNNFYRRSS 105
>SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 455
Score = 24.6 bits (51), Expect = 9.4
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -2
Query: 483 FYSLPYRVFSVQDPNVADDRQHFVRSL 403
+YS P +F Q P +A+ Q F+R L
Sbjct: 108 WYSKPILIFLHQTPELAEASQKFLRYL 134
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,843,525
Number of Sequences: 5004
Number of extensions: 34357
Number of successful extensions: 89
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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