Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31464
(632 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 56 6e-09
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 54 2e-08
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 42 1e-04
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 32 0.079
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 28 1.3
SPBC1709.10c |||metallochaperone |Schizosaccharomyces pombe|chr ... 27 1.7
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 27 2.3
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 6.9
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 25 6.9
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 6.9
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 25 9.1
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 25 9.1
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 9.1
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 55.6 bits (128), Expect = 6e-09
Identities = 33/104 (31%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
Frame = +1
Query: 178 WHSGHFCCWQCDESLTGQRYVL---RDEHPYCIKCYESVFANGCEECNKIIGIDSKDLSY 348
WH FCC +CD+ L +L D P C C A+ C C I D +S
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIK-DYALMSG 93
Query: 349 KDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASR 480
D + H CF C CR ++D F I+C +C + SR
Sbjct: 94 YDSY-HRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
Score = 31.9 bits (69), Expect = 0.079
Identities = 26/106 (24%), Positives = 37/106 (34%), Gaps = 3/106 (2%)
Frame = +1
Query: 298 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKLD-KIYCGNCYDAQ 468
C C + +S+ WH+ CF C KC L D+ D + C +C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74
Query: 469 FASRCDGCGEVFRAGTKKMGVQDQGSGTEKCFCCVVCKNPIGTKSF 606
A C C + G S +CF C C+ I +F
Sbjct: 75 -AHTCTACRMRIKDYALMSGYD---SYHRECFRCHDCRKQIIDSNF 116
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 54.0 bits (124), Expect = 2e-08
Identities = 39/157 (24%), Positives = 65/157 (41%), Gaps = 4/157 (2%)
Frame = +1
Query: 148 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKCYESVFANGCEECNKIIGI 327
G A K H F C C ++L + R+ YC Y F+ C+ C I
Sbjct: 268 GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCK--TPI 325
Query: 328 DSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVF 504
+ + + + +HE CA C V V+ + D +C CYD ++A +C C +
Sbjct: 326 EDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPI 385
Query: 505 RAGTKKMGVQDQGSGTE---KCFCCVVCKNPIGTKSF 606
+G+ +GS E +C+ C C +G + +
Sbjct: 386 ------LGISVKGSDGEYHSQCWTCGACNALLGDEGY 416
Score = 37.9 bits (84), Expect = 0.001
Identities = 21/73 (28%), Positives = 27/73 (36%)
Frame = +1
Query: 274 YESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGN 453
Y C C + + +S K H CF C C +L F + K YC
Sbjct: 250 YRGNSEKSCHSCGGSLRA-GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 454 CYDAQFASRCDGC 492
Y QF+ RC C
Sbjct: 309 DYHEQFSPRCKHC 321
Score = 25.8 bits (54), Expect = 5.2
Identities = 11/42 (26%), Positives = 19/42 (45%)
Frame = +1
Query: 148 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKC 273
G K + ++HS + C C+ L + Y + + P C C
Sbjct: 387 GISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 41.5 bits (93), Expect = 1e-04
Identities = 31/127 (24%), Positives = 53/127 (41%), Gaps = 7/127 (5%)
Frame = +1
Query: 148 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRD-----EHPYCIKCYESVFANGCEECN 312
G+Y +A+ +H F C C+ + + + + D + P C Y C C
Sbjct: 125 GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCG 184
Query: 313 KIIGIDSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDG 489
+ + ++ +K +H F C+ C V + + K+YC Y FA+RC G
Sbjct: 185 --MALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCG 242
Query: 490 C-GEVFR 507
C G + R
Sbjct: 243 CDGPILR 249
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 31.9 bits (69), Expect = 0.079
Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 3/81 (3%)
Frame = +1
Query: 259 YCIKCYESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKL 432
+CIKC+ESV + + + + K WH CF C C L + F
Sbjct: 23 FCIKCWESVPS-------------TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69
Query: 433 DK-IYCGNCYDAQFASRCDGC 492
K I+C C D C+GC
Sbjct: 70 QKQIFCKLCVDI-----CNGC 85
Score = 27.9 bits (59), Expect = 1.3
Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 3/102 (2%)
Frame = +1
Query: 172 KDWHSGHFCCWQCDESL--TGQRYVLRDEHP-YCIKCYESVFANGCEECNKIIGIDSKDL 342
K WHS F C C++ L + + + D+ +C C + NGC I +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD--ICNGCS--TPICEFN---- 93
Query: 343 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQ 468
+ + + C C+ CR + FG + YC C Q
Sbjct: 94 AVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQ 133
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +1
Query: 187 GHFCCWQCDESLTGQRYVLRDEHPYCIKC 273
GHF C +C L GQ+Y KC
Sbjct: 623 GHFTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPBC1709.10c |||metallochaperone |Schizosaccharomyces pombe|chr
2|||Manual
Length = 68
Score = 27.5 bits (58), Expect = 1.7
Identities = 13/44 (29%), Positives = 17/44 (38%)
Frame = +1
Query: 457 YDAQFASRCDGCGEVFRAGTKKMGVQDQGSGTEKCFCCVVCKNP 588
Y A CDGC ++GV+D+ EK V P
Sbjct: 3 YQFNVAMACDGCKNAIDRVLTRLGVEDKSISVEKQEVIVTTDKP 46
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 27.1 bits (57), Expect = 2.3
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -1
Query: 410 STRETRHLAHKKQASCQCLSLYDRSLESMPMILLHSSQPLAKTLS 276
S R T A KQA L+ Y +++M IL + +PLA T S
Sbjct: 753 SPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRS 797
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 25.4 bits (53), Expect = 6.9
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +1
Query: 346 YKDKHWHEACFLCAKCRVSLVDK 414
Y H C++CAKC+ L K
Sbjct: 4 YYPVHLKSRCYVCAKCKTHLAFK 26
>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 638
Score = 25.4 bits (53), Expect = 6.9
Identities = 12/33 (36%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
Frame = +1
Query: 274 YESVFANGCEECNKIIGIDSKDLSYKDKH--WH 366
YE+ ++N C C + G D + DKH WH
Sbjct: 416 YEN-YSNQCANCGRRYGNDPESRKELDKHSDWH 447
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 6.9
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -3
Query: 438 LVELGAELLVDQRDAALGAQEAGLVPVLVLVRQILRVNADD 316
L LG ELL + D Q A L+ V+ ++ L A+D
Sbjct: 689 LTPLGMELLQNYIDHTSDVQTAALIAAFVVPKKFLDKRAED 729
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 25.0 bits (52), Expect = 9.1
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = -1
Query: 386 AHKKQASCQCLSLYDRSLESMPMILL 309
A + ++S Q LSL R+ E MP+++L
Sbjct: 363 AIESESSSQSLSLSQRASEVMPLVIL 388
>SPCC126.07c |||human CTD-binding SR-like protein rA9
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 571
Score = 25.0 bits (52), Expect = 9.1
Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 6/90 (6%)
Frame = +1
Query: 253 HPYCIKCYESVFANGCEECN-KIIGIDSKDLSYKDKHWHEACFLCAKCR-VSLVDKQF-- 420
H +C++ + V AN C C + + +D L + W+ A + K + V+ + F
Sbjct: 61 HNHCLESWCRV-ANTCPLCRTEFLKVDV--LEFVKGPWYRAYPVEEKTQSVANAGEPFED 117
Query: 421 -GSKLDK-IYCGNCYDAQFASRCDGCGEVF 504
GS+ + + CG A+ CDGC + +
Sbjct: 118 EGSETCRCVICGRSDHAEVLLLCDGCDDAY 147
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 25.0 bits (52), Expect = 9.1
Identities = 10/23 (43%), Positives = 12/23 (52%), Gaps = 1/23 (4%)
Frame = +1
Query: 445 CGNC-YDAQFASRCDGCGEVFRA 510
C C YD +CDGCG + A
Sbjct: 363 CPKCGYDDARGDQCDGCGGLLNA 385
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,939,331
Number of Sequences: 5004
Number of extensions: 34834
Number of successful extensions: 141
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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