Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31435
(581 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26750.1 68417.m03854 hydroxyproline-rich glycoprotein family... 33 0.18
At5g67070.1 68418.m08457 rapid alkalinization factor (RALF) fami... 31 0.42
At1g21310.1 68414.m02662 proline-rich extensin-like family prote... 31 0.56
At3g63070.1 68416.m07084 PWWP domain-containing protein putative... 30 1.3
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 29 2.3
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 29 2.3
At3g54580.1 68416.m06039 proline-rich extensin-like family prote... 29 3.0
At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal hydrolase-r... 28 4.0
At4g14150.1 68417.m02183 phragmoplast-associated kinesin-related... 28 5.2
At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family... 27 6.9
At2g24590.1 68415.m02936 splicing factor, putative similar to to... 27 6.9
At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase cyc... 27 9.1
At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing ... 27 9.1
At2g36220.1 68415.m04446 expressed protein 27 9.1
>At4g26750.1 68417.m03854 hydroxyproline-rich glycoprotein family
protein
Length = 421
Score = 32.7 bits (71), Expect = 0.18
Identities = 27/121 (22%), Positives = 41/121 (33%)
Frame = +3
Query: 144 PPPRDAPYMRDRPGPVRGYERGPPAAPYDERYAYGEDRRGFALMDRGPRDXXXXXXXXXX 323
PPP P P P GP +PY Y++ + P++
Sbjct: 234 PPPSSYPSNDHLPPPT-----GPSDSPYPHPYSHQPYHQDPPKHMPPPQNYSSHEPSPNS 288
Query: 324 DERYQNHISVVEGTRRYDEPAYDDRHYDERAQYERPRPAHHIPNGDMYSRRSPAPMYSGR 503
+Q++ S E + P Y + + Y P A P+ +S P P YS
Sbjct: 289 LPNFQSYPSFSESSLPSTSPHYPSHYQNPEPYYSSPHSAP-APSSTSFSSAPPPPPYSSN 347
Query: 504 G 506
G
Sbjct: 348 G 348
>At5g67070.1 68418.m08457 rapid alkalinization factor (RALF) family
protein similar to RALF precursor [Nicotiana tabacum]
GI:16566316
Length = 129
Score = 31.5 bits (68), Expect = 0.42
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +3
Query: 333 YQNHISVVEGTRRYDEPAYDDRHYDERAQYERPRPAHHIPNGDMYSRRSPAPMYSGR 503
+ + +++G ++ DD D R+ Y R R ++I G + + R P P SGR
Sbjct: 45 HSDEFDIIDGEESFEVTEEDDGVTDRRSLYWR-RTKYYISYGALSANRVPCPPRSGR 100
>At1g21310.1 68414.m02662 proline-rich extensin-like family protein
contains extensin-like region, Pfam:PF04554
Length = 431
Score = 31.1 bits (67), Expect = 0.56
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +3
Query: 129 PVRHAPPPRDAPYMRDRPGPVRGYERGPPAAPY 227
PV H+PPP Y+ P P + PP PY
Sbjct: 388 PVYHSPPPPKEKYVYKSPPPPPVHHYSPPHHPY 420
Score = 29.1 bits (62), Expect = 2.3
Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 4/43 (9%)
Frame = +3
Query: 129 PVRHAPPPRDAPYM-RDRPGPVRGYERGP---PAAPYDERYAY 245
PV H+PPP Y+ + P PV+ Y P P E+Y Y
Sbjct: 360 PVYHSPPPPKKHYVYKSPPPPVKHYSPPPVYHSPPPPKEKYVY 402
Score = 27.1 bits (57), Expect = 9.1
Identities = 17/63 (26%), Positives = 24/63 (38%), Gaps = 3/63 (4%)
Frame = +3
Query: 372 YDEPAYDDRHYDERAQYERPRPAHHIPNGDM--YSRRSPAPMYSGRGGPPAV-AGGYERD 542
Y P +HY ++ P P +H P Y +SP P PP + +
Sbjct: 31 YSSPPPPVKHYTPPVKHYSPPPVYHSPPPPKKHYEYKSPPPPVKHYSPPPVYHSPPPPKK 90
Query: 543 HYV 551
HYV
Sbjct: 91 HYV 93
>At3g63070.1 68416.m07084 PWWP domain-containing protein putative
transcription factor HUA2, Arabidopsis thaliana,
EMBL:AF116556
Length = 1347
Score = 29.9 bits (64), Expect = 1.3
Identities = 14/53 (26%), Positives = 22/53 (41%)
Frame = +3
Query: 387 YDDRHYDERAQYERPRPAHHIPNGDMYSRRSPAPMYSGRGGPPAVAGGYERDH 545
+D+R+Y + + RP P + N + S P Y R P + Y H
Sbjct: 1248 FDERNYQDSYERMRPEPCENRDNWRYHPPSSHGPRYHDRHKGPHQSSSYSGHH 1300
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 29.1 bits (62), Expect = 2.3
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = +3
Query: 138 HAPPPRDAPYMRDRPGPVRGYERGPPAAP 224
H+PPP Y P P E PP AP
Sbjct: 680 HSPPPSPVHYSSPPPPPSAPCEESPPPAP 708
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 29.1 bits (62), Expect = 2.3
Identities = 24/75 (32%), Positives = 28/75 (37%)
Frame = -3
Query: 411 ARRSGGRHRQAHRNVAYPPPLRCDSGSVRQGKQAPAVHKRP*GRDPSARTHAGPRRTHSA 232
ARR A R + PP R R P +R P AR H P
Sbjct: 317 ARRRRSPSPPARRRRSPSPPAR------RHRSPTPPARQRR-SPSPPARRHRSPPPARRR 369
Query: 231 RHRAPPADRVRSRAP 187
R +PPA R RS +P
Sbjct: 370 RSPSPPARRRRSPSP 384
>At3g54580.1 68416.m06039 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 951
Score = 28.7 bits (61), Expect = 3.0
Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 3/53 (5%)
Frame = +3
Query: 420 YERPRPAHHIPNGDMYSRRSPAPMYSGRGGPPAVAGGYERDHY---VPQYMQP 569
Y P P H+ P+ +Y +SP P Y PP + HY P Y P
Sbjct: 719 YSSPPPPHYSPSPKVY-YKSPPPPYVYSSPPPPYYSPSPKVHYKSPPPPYYAP 770
>At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of unknown
function (DUF627)
Length = 1086
Score = 28.3 bits (60), Expect = 4.0
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +1
Query: 490 CIPGVEVHLQWPEVTREITMYHNTCNLHWD 579
C P + L+W + EI +Y T LHW+
Sbjct: 971 CPPIFTIVLEWEKNETEIEIYETTNALHWE 1000
>At4g14150.1 68417.m02183 phragmoplast-associated kinesin-related
protein (PAKRP1)
Length = 1292
Score = 27.9 bits (59), Expect = 5.2
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = -3
Query: 291 P*GRDPSARTHAGPRRTHSARHRAPPADRVRSRAPD 184
P +PS PR+ SA+ APP DR + PD
Sbjct: 19 PHSPNPSISKSKPPRKLRSAKENAPPLDR-NTSTPD 53
>At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 205
Score = 27.5 bits (58), Expect = 6.9
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +3
Query: 129 PVRHAPPPRDAPYMRDRPGPVRGYERGPPAAPY 227
P H PPP +P + P P + PP +PY
Sbjct: 40 PPHHPPPPHFSPPHQPPPSPY-PHPHPPPPSPY 71
>At2g24590.1 68415.m02936 splicing factor, putative similar to to
RSZp22 protein [Arabidopsis thaliana]
gi|2582645|emb|CAA05352
Length = 196
Score = 27.5 bits (58), Expect = 6.9
Identities = 24/88 (27%), Positives = 31/88 (35%), Gaps = 1/88 (1%)
Frame = -3
Query: 447 CGEQVSGVHTVRARRSGGRHRQAHRNVAYPPPLRCDSGSVRQGKQAPAVHKRP*GRDPSA 268
CGE R+R G R++ PP R + +P P P
Sbjct: 101 CGESGHFARECRSRGGSGGRRRSRSRSRSPPRYRKSPTYGGRRSYSPRARSPP----PPR 156
Query: 267 RTHAGPR-RTHSARHRAPPADRVRSRAP 187
R PR R +S R+PP R R P
Sbjct: 157 RRSPSPRGRNYS---RSPPPYRARDEVP 181
>At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase
cyclophilin-type family protein weak similarity to
CARS-Cyp [Homo sapiens] GI:1117968; contains Pfam
profile PF00160: peptidyl-prolyl cis-trans isomerase,
cyclophilin-type
Length = 837
Score = 27.1 bits (57), Expect = 9.1
Identities = 29/100 (29%), Positives = 46/100 (46%), Gaps = 4/100 (4%)
Frame = -3
Query: 432 SGVHTVRARRSGGRHRQAHRNVAYPPPLRCDSGSVRQGK---QAPAVHKRP*-GRDPSAR 265
S V + R R S R + ++V+ P +R S+ + ++ + P GR +R
Sbjct: 586 SPVRSSRRRISRSPVRSSRKSVSRSP-IRLSRRSISRSPIRLSRRSISRSPVRGRRRISR 644
Query: 264 THAGPRRTHSARHRAPPADRVRSRAPDLAGPSCMVRPEAG 145
+ P R S R R+PP DR RS + A P+ +R G
Sbjct: 645 SPV-PARRRSVRPRSPPPDRRRSLSRS-ASPNGRIRRGRG 682
>At2g44710.1 68415.m05564 RNA recognition motif (RRM)-containing
protein
Length = 809
Score = 27.1 bits (57), Expect = 9.1
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = +3
Query: 135 RHAPPPRDAPYMRDRPGPVRGYERGPPAAPY 227
R PPP A R P P R Y+R PP Y
Sbjct: 559 RPMPPPARA---RPLPPPARSYDRRPPVPLY 586
>At2g36220.1 68415.m04446 expressed protein
Length = 263
Score = 27.1 bits (57), Expect = 9.1
Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 2/76 (2%)
Frame = -3
Query: 438 QVSGVHTVRARRSGGRHRQAHRNVAYPPPLRCDSGSVRQGKQAPAVHKRP*G-RDPSAR- 265
+VS + VR+RR R ++ P R +A + + G +D SAR
Sbjct: 151 KVSCIGRVRSRRERSRRMHRQKSSRVEPVNRVKKPGFMASFRA--IFRIKGGCKDVSARE 208
Query: 264 THAGPRRTHSARHRAP 217
TH R TH R R P
Sbjct: 209 THTSTRNTHDIRSRLP 224
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,915,249
Number of Sequences: 28952
Number of extensions: 249946
Number of successful extensions: 1054
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 783
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1038
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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