BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31422 (755 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 454 e-129 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 259 3e-70 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 248 5e-67 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 171 1e-43 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 120 2e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 113 3e-26 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 100 3e-22 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 76 6e-15 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 68 1e-12 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.18 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.31 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.8 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 5.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 454 bits (1120), Expect = e-129 Identities = 208/230 (90%), Positives = 223/230 (96%) Frame = +2 Query: 65 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 244 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 245 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 424 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 425 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 604 I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181 Query: 605 GRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEQEMGTAA 754 GRDLTDYLMKIL ERGY+F+TTAE EIVRDIKEKLCYVALDFEQE+ TAA Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAA 231 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 259 bits (635), Expect = 3e-70 Identities = 119/225 (52%), Positives = 163/225 (72%), Gaps = 1/225 (0%) Frame = +2 Query: 71 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 250 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 251 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 427 +L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 428 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 607 +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187 Query: 608 RDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEQEM 742 RD+TDYL L + G+ ++AE EIVR+IKEK CYVA DF E+ Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 248 bits (608), Expect = 5e-67 Identities = 115/221 (52%), Positives = 160/221 (72%), Gaps = 2/221 (0%) Frame = +2 Query: 77 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 250 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 251 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 430 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 431 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 610 FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGR Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183 Query: 611 DLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFE 733 D T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E Sbjct: 184 DATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLE 224 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 171 bits (415), Expect = 1e-43 Identities = 98/251 (39%), Positives = 139/251 (55%), Gaps = 32/251 (12%) Frame = +2 Query: 83 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 211 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 212 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 370 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 371 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 526 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186 Query: 527 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEK 706 GDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R + + IKE+ Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRN---EPDSSLKTAERIKEE 243 Query: 707 LCYVALDFEQE 739 CYV D +E Sbjct: 244 CCYVCPDIVKE 254 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 120 bits (289), Expect = 2e-28 Identities = 72/252 (28%), Positives = 132/252 (52%), Gaps = 3/252 (1%) Frame = +2 Query: 5 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 184 A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60 Query: 185 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 361 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + E Sbjct: 61 K----LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115 Query: 362 HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDG 535 HP+ +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ Sbjct: 116 HPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNA 175 Query: 536 VSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCY 715 SH +P+ G + R+ G + YL+K+ + SF + C+ Sbjct: 176 ASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCH 235 Query: 716 VALDFEQEMGTA 751 V+ D+ ++ A Sbjct: 236 VSSDYTHDIAHA 247 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 113 bits (271), Expect = 3e-26 Identities = 63/196 (32%), Positives = 101/196 (51%) Frame = +2 Query: 68 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 247 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 248 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 427 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 428 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 607 MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180 Query: 608 RDLTDYLMKILTERGY 655 + D L + L ++ + Sbjct: 181 NAINDILAQTLRDKNF 196 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 100 bits (239), Expect = 3e-22 Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%) Frame = +2 Query: 68 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 247 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 248 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 421 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 422 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 601 + FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I + L Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181 Query: 602 AGRDLTDYLMKILTERGYSF 661 AG L + ++ F Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 75.8 bits (178), Expect = 6e-15 Identities = 62/238 (26%), Positives = 110/238 (46%), Gaps = 18/238 (7%) Frame = +2 Query: 83 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 262 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 263 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 430 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 431 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 568 FE F A A L + G T +V+DSG +H +P+ + Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177 Query: 569 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEQEM 742 A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+ +F+++M Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNFKEDM 233 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 68.1 bits (159), Expect = 1e-12 Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%) Frame = +2 Query: 269 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 436 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 437 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 616 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR + Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 617 TDYLMKILTERGYSFTTTAEXEIVRDIKE 703 T +L +L E + ++ + DI E Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.9 bits (84), Expect = 0.002 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%) Frame = +2 Query: 260 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 403 L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L + Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257 Query: 404 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 583 EK+ I+F + + +++ + + +G + V+D G + + EG +P++ Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317 Query: 584 ILRLDLAGRDLTDYLMKILTERGYSF 661 ++++ G D+T MK+L + + Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.18 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 128 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 271 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.31 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 329 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 472 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.8 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +3 Query: 555 STKVTLCPTPSSVWTW 602 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 5.0 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 371 LLTEAPLNPKANREKMTQIMFETFN 445 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,887,461 Number of Sequences: 5004 Number of extensions: 58461 Number of successful extensions: 219 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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