Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31399
(807 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 2e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 57 3e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 32 0.11
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 28 1.8
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.1
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 7.2
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 26 7.2
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 9.6
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 25 9.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.9 bits (166), Expect = 2e-13
Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
Frame = +3
Query: 42 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 215
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 216 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 392
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 393 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 485
EVA++ ++ D DG+I Y F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 2e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +3
Query: 84 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 263
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 264 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 443
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 444 DDGMIPYAAFLKKVMA 491
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 57.2 bits (132), Expect = 3e-09
Identities = 37/146 (25%), Positives = 71/146 (48%)
Frame = +3
Query: 54 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 233
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 234 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 413
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 414 VTKDCMDPEDDDGMIPYAAFLKKVMA 491
+ ++ DP + G Y F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.9 bits (69), Expect = 0.11
Identities = 33/161 (20%), Positives = 63/161 (39%), Gaps = 1/161 (0%)
Frame = +3
Query: 9 RPGGNRQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 188
R GG ++++ + D+ A F ++D + ID L +RAL N + +
Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76
Query: 189 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 365
F+ + ++ ++D E+ +L+D +E G + L
Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134
Query: 366 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 488
L E +DD E+ + ++ D DG I F+ +M
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 27.9 bits (59), Expect = 1.8
Identities = 22/97 (22%), Positives = 44/97 (45%), Gaps = 2/97 (2%)
Frame = +1
Query: 16 EGTD-KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSR 189
E TD ++HT T A+ KG P++ KAK+ + ++ +S Q P++
Sbjct: 38 ERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTK 97
Query: 190 NSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 300
+S+ + + + SK S+ ++ + +IS
Sbjct: 98 HSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.1
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +3
Query: 42 MSDLSKNDVER-ASFAFSIYDFEGKGKID 125
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 7.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 378 LGEKLDDSEVAEVTKDCMDPED 443
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1325
Score = 25.8 bits (54), Expect = 7.2
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 577 KPGTEEPAPGSARGGLSSFRWRPQPA 654
+P TE+ G G S+F+ +PQP+
Sbjct: 672 EPSTEQNIGGDVSWGASTFQSKPQPS 697
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 9.6
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +1
Query: 61 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 234
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 235 KSSFPSTAKQRKTKT 279
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 9.6
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = +1
Query: 586 TEEPAPGSAR--GGLSSFRWRPQPARAPSHVDIPYNILNTDKP 708
T P P S R GGL++ RP P A S + +P KP
Sbjct: 372 TAAPPPPSRRKTGGLNT---RPYPQHAESQMSLPLTAKERSKP 411
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,256,268
Number of Sequences: 5004
Number of extensions: 65867
Number of successful extensions: 191
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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