BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31393 (780 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.3 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.3 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.0 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 7.0 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 7.0 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 7.0 SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 9.2 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.2 SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 9.2 SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 9.2 >SPAC1006.07 |||translation initiation factor eIF4A|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 28.3 bits (60), Expect = 1.3 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +2 Query: 224 AIDLLTNDECRLLLEVEDFFN 286 +I+ +TND+ R++ E+E F+N Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 27.5 bits (58), Expect = 2.3 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +3 Query: 357 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 446 STR + N+VP N L I+C + NK Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 27.1 bits (57), Expect = 3.0 Identities = 19/71 (26%), Positives = 29/71 (40%), Gaps = 2/71 (2%) Frame = +2 Query: 488 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSP- 664 P+ S V P P A++ AS+P + S P ++ + T + P Q P Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952 Query: 665 -PGPADSVASE 694 P P VA + Sbjct: 953 HPNPYTPVAPQ 963 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 25.8 bits (54), Expect = 7.0 Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Frame = +2 Query: 467 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 619 +E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+ Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 25.8 bits (54), Expect = 7.0 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Frame = -2 Query: 641 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 507 A T H P + P+ +QW +S +DL+ ++ V+ +E K Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 25.8 bits (54), Expect = 7.0 Identities = 16/65 (24%), Positives = 32/65 (49%) Frame = -2 Query: 689 KQLSPRVPADSAAVLNALTRQHHKPFRIPAAVLQWAARSQKDLKLEPLPGVVGPRVQELQ 510 K+L+ + A +NA +H ++ A ++ + K + E L V+G R+ L+ Sbjct: 629 KKLAEELKAKGGLEVNAEDLEHLDADKLRAMQIEQVEKQNKSMN-ERLR-VIGKRIDHLE 686 Query: 509 KCFRR 495 + +RR Sbjct: 687 RAYRR 691 >SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 731 Score = 25.4 bits (53), Expect = 9.2 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +2 Query: 425 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 541 +D CKQ+ +FS E++ D L SI +S + ++ P Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 9.2 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +2 Query: 242 NDECRLLLEVEDFFNDDC 295 NDECR L + FF+ +C Sbjct: 535 NDECRRLKQCNHFFHREC 552 >SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2310 Score = 25.4 bits (53), Expect = 9.2 Identities = 14/49 (28%), Positives = 23/49 (46%) Frame = -3 Query: 367 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 221 W T+ V R S RKV + + + EE +++ ALI + + R Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191 >SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual Length = 304 Score = 25.4 bits (53), Expect = 9.2 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Frame = +1 Query: 73 LPKWRPHRXKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 207 L ++ + KLV+L T T LD + ++ A V+ N Y N C+++ Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,869,791 Number of Sequences: 5004 Number of extensions: 53939 Number of successful extensions: 143 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -