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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31393
         (780 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar...    28   1.3  
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    27   2.3  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    27   3.0  
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb...    26   7.0  
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch...    26   7.0  
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos...    26   7.0  
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos...    25   9.2  
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   9.2  
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce...    25   9.2  
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual    25   9.2  

>SPAC1006.07 |||translation initiation factor
           eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 392

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 9/21 (42%), Positives = 17/21 (80%)
 Frame = +2

Query: 224 AIDLLTNDECRLLLEVEDFFN 286
           +I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = +3

Query: 357 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 446
           STR   +  N+VP N L I+C    +  NK
Sbjct: 74  STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 19/71 (26%), Positives = 29/71 (40%), Gaps = 2/71 (2%)
 Frame = +2

Query: 488  PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSP- 664
            P+     S V     P P A++ AS+P + S P  ++   +         T + P Q P 
Sbjct: 895  PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952

Query: 665  -PGPADSVASE 694
             P P   VA +
Sbjct: 953  HPNPYTPVAPQ 963


>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
           membrane proteins, ESCRT 0 complex|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 610

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = +2

Query: 467 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 619
           +E + D+ S   N S +P+      P  V+ SSP   + P+  +++ + +T+
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521


>SPCC417.08 |tef3||translation elongation factor
           eEF3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1047

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
 Frame = -2

Query: 641 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 507
           A T   H P + P+  +QW  +S +DL+ ++    V+    +E  K
Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791


>SPBC17D11.05 |tif32||translation initiation factor
           eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 932

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 16/65 (24%), Positives = 32/65 (49%)
 Frame = -2

Query: 689 KQLSPRVPADSAAVLNALTRQHHKPFRIPAAVLQWAARSQKDLKLEPLPGVVGPRVQELQ 510
           K+L+  + A     +NA   +H    ++ A  ++   +  K +  E L  V+G R+  L+
Sbjct: 629 KKLAEELKAKGGLEVNAEDLEHLDADKLRAMQIEQVEKQNKSMN-ERLR-VIGKRIDHLE 686

Query: 509 KCFRR 495
           + +RR
Sbjct: 687 RAYRR 691


>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 731

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +2

Query: 425 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 541
           +D  CKQ+ +FS   E++ D L SI   +S +  ++   P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281


>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 583

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = +2

Query: 242 NDECRLLLEVEDFFNDDC 295
           NDECR L +   FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552


>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2310

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 14/49 (28%), Positives = 23/49 (46%)
 Frame = -3

Query: 367  WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 221
            W   T+ V     R S RKV + + +  EE    +++ ALI  + +  R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191


>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 304

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +1

Query: 73  LPKWRPHRXKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 207
           L ++   + KLV+L      T T LD +   ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,869,791
Number of Sequences: 5004
Number of extensions: 53939
Number of successful extensions: 143
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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