BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31393
(780 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.3
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.3
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.0
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 7.0
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 7.0
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 7.0
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 9.2
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.2
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 9.2
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 9.2
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 1.3
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +2
Query: 224 AIDLLTNDECRLLLEVEDFFN 286
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 2.3
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 357 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 446
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 3.0
Identities = 19/71 (26%), Positives = 29/71 (40%), Gaps = 2/71 (2%)
Frame = +2
Query: 488 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSP- 664
P+ S V P P A++ AS+P + S P ++ + T + P Q P
Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952
Query: 665 -PGPADSVASE 694
P P VA +
Sbjct: 953 HPNPYTPVAPQ 963
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 467 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 619
+E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 641 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 507
A T H P + P+ +QW +S +DL+ ++ V+ +E K
Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.8 bits (54), Expect = 7.0
Identities = 16/65 (24%), Positives = 32/65 (49%)
Frame = -2
Query: 689 KQLSPRVPADSAAVLNALTRQHHKPFRIPAAVLQWAARSQKDLKLEPLPGVVGPRVQELQ 510
K+L+ + A +NA +H ++ A ++ + K + E L V+G R+ L+
Sbjct: 629 KKLAEELKAKGGLEVNAEDLEHLDADKLRAMQIEQVEKQNKSMN-ERLR-VIGKRIDHLE 686
Query: 509 KCFRR 495
+ +RR
Sbjct: 687 RAYRR 691
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +2
Query: 425 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 541
+D CKQ+ +FS E++ D L SI +S + ++ P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 9.2
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2
Query: 242 NDECRLLLEVEDFFNDDC 295
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 9.2
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -3
Query: 367 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 221
W T+ V R S RKV + + + EE +++ ALI + + R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 9.2
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +1
Query: 73 LPKWRPHRXKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 207
L ++ + KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,869,791
Number of Sequences: 5004
Number of extensions: 53939
Number of successful extensions: 143
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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