BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31383 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 413 e-116 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 231 8e-62 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 223 2e-59 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 164 1e-41 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 116 3e-27 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 113 3e-26 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 100 2e-22 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 67 3e-12 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 56 4e-09 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.001 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.17 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.29 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.6 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 413 bits (1016), Expect = e-116 Identities = 187/206 (90%), Positives = 201/206 (97%) Frame = +1 Query: 58 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 237 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 238 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 417 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 418 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 597 I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181 Query: 598 GRDLTDYLMKILTERGYSFTTTAERE 675 GRDLTDYLMKIL ERGY+F+TTAERE Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAERE 207 Score = 37.5 bits (83), Expect = 0.002 Identities = 16/17 (94%), Positives = 17/17 (100%) Frame = +2 Query: 674 KIVRDIKEKLCYVALDF 724 +IVRDIKEKLCYVALDF Sbjct: 207 EIVRDIKEKLCYVALDF 223 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 231 bits (565), Expect = 8e-62 Identities = 105/205 (51%), Positives = 148/205 (72%), Gaps = 1/205 (0%) Frame = +1 Query: 64 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 243 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 244 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 420 +L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 421 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 600 +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187 Query: 601 RDLTDYLMKILTERGYSFTTTAERE 675 RD+TDYL L + G+ ++AE+E Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKE 212 Score = 31.5 bits (68), Expect = 0.13 Identities = 13/17 (76%), Positives = 15/17 (88%) Frame = +2 Query: 674 KIVRDIKEKLCYVALDF 724 +IVR+IKEK CYVA DF Sbjct: 212 EIVREIKEKCCYVASDF 228 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 223 bits (545), Expect = 2e-59 Identities = 104/204 (50%), Positives = 146/204 (71%), Gaps = 2/204 (0%) Frame = +1 Query: 70 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 243 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 244 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 423 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 424 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 603 FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGR Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183 Query: 604 DLTDYLMKILTERGYSFTTTAERE 675 D T YL+ +L +GY+F TA+ E Sbjct: 184 DATRYLISLLLRKGYAFNRTADFE 207 Score = 27.1 bits (57), Expect = 2.7 Identities = 10/14 (71%), Positives = 13/14 (92%) Frame = +2 Query: 680 VRDIKEKLCYVALD 721 VR++KEKLCYV+ D Sbjct: 209 VREMKEKLCYVSYD 222 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 164 bits (398), Expect = 1e-41 Identities = 95/233 (40%), Positives = 132/233 (56%), Gaps = 34/233 (14%) Frame = +1 Query: 76 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 204 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 205 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 363 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 364 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 519 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186 Query: 520 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 672 GDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 116 bits (279), Expect = 3e-27 Identities = 67/219 (30%), Positives = 121/219 (55%), Gaps = 3/219 (1%) Frame = +1 Query: 7 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 186 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61 Query: 187 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 363 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ Sbjct: 62 --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118 Query: 364 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 537 +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178 Query: 538 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 654 +P+ G + R+ G + YL+K+ + SF Sbjct: 179 VIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSF 217 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 113 bits (271), Expect = 3e-26 Identities = 63/196 (32%), Positives = 101/196 (51%) Frame = +1 Query: 61 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 240 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 241 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 420 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 421 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 600 MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180 Query: 601 RDLTDYLMKILTERGY 648 + D L + L ++ + Sbjct: 181 NAINDILAQTLRDKNF 196 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 100 bits (239), Expect = 2e-22 Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%) Frame = +1 Query: 61 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 240 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 241 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 414 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 415 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 594 + FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I + L Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181 Query: 595 AGRDLTDYLMKILTERGYSF 654 AG L + ++ F Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 66.9 bits (156), Expect = 3e-12 Identities = 40/130 (30%), Positives = 63/130 (48%), Gaps = 4/130 (3%) Frame = +1 Query: 262 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 429 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 430 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 609 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR + Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 610 TDYLMKILTE 639 T +L +L E Sbjct: 232 TQHLQNLLRE 241 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 56.4 bits (130), Expect = 4e-09 Identities = 51/210 (24%), Positives = 92/210 (43%), Gaps = 18/210 (8%) Frame = +1 Query: 76 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 255 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 256 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 423 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 424 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 561 FE F A A L + G T +V+DSG +H +P+ + Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177 Query: 562 ALPHAILRLDLAGRDLTDYLMKILTERGYS 651 A+ R+D+ G+ LT+YL ++++ R Y+ Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.9 bits (84), Expect = 0.001 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%) Frame = +1 Query: 253 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 396 L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L + Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257 Query: 397 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 576 EK+ I+F + + +++ + + +G + V+D G + + EG +P++ Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317 Query: 577 ILRLDLAGRDLTDYLMKILTERGYSF 654 ++++ G D+T MK+L + + Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.17 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 121 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 264 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.29 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 322 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 465 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.6 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 548 STKVTLCPTPSSVWTW 595 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 364 LLTEAPLNPKANREKMTQIMFETFN 438 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,807,777 Number of Sequences: 5004 Number of extensions: 56774 Number of successful extensions: 222 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 213 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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