Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31362
(736 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 411 e-116
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 229 3e-61
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 222 4e-59
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 164 1e-41
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 116 3e-27
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 113 3e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 100 2e-22
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 67 3e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 56 4e-09
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.001
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.17
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.30
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.7
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.8
SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 25 8.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 411 bits (1011), Expect = e-116
Identities = 186/205 (90%), Positives = 200/205 (97%)
Frame = +2
Query: 65 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 244
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 245 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 424
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 425 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 604
I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181
Query: 605 GRDLTDYLMKILTERGYSFTTTAER 679
GRDLTDYLMKIL ERGY+F+TTAER
Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAER 206
Score = 39.9 bits (89), Expect = 4e-04
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = +3
Query: 681 EIVRDIKEKLCYVALDFQ 734
EIVRDIKEKLCYVALDF+
Sbjct: 207 EIVRDIKEKLCYVALDFE 224
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 229 bits (560), Expect = 3e-61
Identities = 104/204 (50%), Positives = 147/204 (72%), Gaps = 1/204 (0%)
Frame = +2
Query: 71 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 250
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 251 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 427
+L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ +
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127
Query: 428 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 607
+ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187
Query: 608 RDLTDYLMKILTERGYSFTTTAER 679
RD+TDYL L + G+ ++AE+
Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEK 211
Score = 33.5 bits (73), Expect = 0.032
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +3
Query: 681 EIVRDIKEKLCYVALDFQ 734
EIVR+IKEK CYVA DF+
Sbjct: 212 EIVREIKEKCCYVASDFR 229
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 222 bits (543), Expect = 4e-59
Identities = 103/202 (50%), Positives = 145/202 (71%), Gaps = 2/202 (0%)
Frame = +2
Query: 77 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 250
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 251 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 430
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123
Query: 431 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 610
FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183
Query: 611 DLTDYLMKILTERGYSFTTTAE 676
D T YL+ +L +GY+F TA+
Sbjct: 184 DATRYLISLLLRKGYAFNRTAD 205
Score = 29.5 bits (63), Expect = 0.52
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +3
Query: 681 EIVRDIKEKLCYVALDFQ 734
E VR++KEKLCYV+ D +
Sbjct: 207 ETVREMKEKLCYVSYDLE 224
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 164 bits (398), Expect = 1e-41
Identities = 95/233 (40%), Positives = 132/233 (56%), Gaps = 34/233 (14%)
Frame = +2
Query: 83 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 211
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 212 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 370
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 371 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 526
LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186
Query: 527 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 679
GDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 116 bits (280), Expect = 3e-27
Identities = 68/222 (30%), Positives = 122/222 (54%), Gaps = 3/222 (1%)
Frame = +2
Query: 5 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 184
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R +
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60
Query: 185 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 361
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + E
Sbjct: 61 K----LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115
Query: 362 HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDG 535
HP+ +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+
Sbjct: 116 HPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNA 175
Query: 536 VSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 661
SH +P+ G + R+ G + YL+K+ + SF
Sbjct: 176 ASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSF 217
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 113 bits (271), Expect = 3e-26
Identities = 63/196 (32%), Positives = 101/196 (51%)
Frame = +2
Query: 68 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 247
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 248 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 427
G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 428 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 607
MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180
Query: 608 RDLTDYLMKILTERGY 655
+ D L + L ++ +
Sbjct: 181 NAINDILAQTLRDKNF 196
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 100 bits (239), Expect = 2e-22
Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%)
Frame = +2
Query: 68 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 247
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 248 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 421
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 422 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 601
+ FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I + L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181
Query: 602 AGRDLTDYLMKILTERGYSF 661
AG L + ++ F
Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 66.9 bits (156), Expect = 3e-12
Identities = 40/130 (30%), Positives = 63/130 (48%), Gaps = 4/130 (3%)
Frame = +2
Query: 269 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 436
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 437 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 616
P +A + ++ LYA G G+V+D G + PI +G + A +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231
Query: 617 TDYLMKILTE 646
T +L +L E
Sbjct: 232 TQHLQNLLRE 241
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 56.4 bits (130), Expect = 4e-09
Identities = 51/210 (24%), Positives = 92/210 (43%), Gaps = 18/210 (8%)
Frame = +2
Query: 83 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 262
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 263 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 430
++ H G + +W +W N + P ++ +LLT+ + Q++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 431 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 568
FE F A A L + G T +V+DSG +H +P+ +
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177
Query: 569 ALPHAILRLDLAGRDLTDYLMKILTERGYS 658
A+ R+D+ G+ LT+YL ++++ R Y+
Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 37.9 bits (84), Expect = 0.001
Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
Frame = +2
Query: 260 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 403
L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L +
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257
Query: 404 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 583
EK+ I+F + + +++ + + +G + V+D G + + EG +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317
Query: 584 ILRLDLAGRDLTDYLMKILTERGYSF 661
++++ G D+T MK+L + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.17
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +2
Query: 128 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 271
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.30
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 329 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 472
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 3.7
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 555 STKVTLCPTPSSVWTW 602
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 371 LLTEAPLNPKANREKMTQIMFETFN 445
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.4 bits (53), Expect = 8.5
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Frame = -1
Query: 694 SRTISP-LSGGGERVTPLGEDLHEVVGQVATSQVQTEDGVGQS 569
S +ISP LSG G +TPL + QV + V QS
Sbjct: 462 SASISPTLSGSGSGLTPLSSHFPSAATGLVGGQVYPQSSVLQS 504
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,847,316
Number of Sequences: 5004
Number of extensions: 57599
Number of successful extensions: 225
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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