Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31361
(491 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P06742 Cluster: Myosin light chain alkali; n=35; Arthro... 70 3e-11
UniRef50_UPI0000D556DD Cluster: PREDICTED: similar to CG5596-PA,... 61 2e-08
UniRef50_P53014 Cluster: Myosin, essential light chain; n=2; Cae... 48 2e-04
UniRef50_P14649 Cluster: Myosin light polypeptide 6B; n=145; Cho... 42 0.006
UniRef50_P12829 Cluster: Myosin light polypeptide 4; n=63; Eutel... 42 0.006
UniRef50_A0A9Q4 Cluster: Myosin light chain; n=5; Chordata|Rep: ... 42 0.007
UniRef50_Q17133 Cluster: Myosin, essential light chain; n=3; Met... 41 0.013
UniRef50_P54357 Cluster: Myosin-2 essential light chain; n=23; E... 38 0.092
UniRef50_P05945 Cluster: Myosin catalytic light chain LC-1, mant... 38 0.12
UniRef50_UPI0000D9C1D1 Cluster: PREDICTED: similar to Calmodulin... 36 0.37
UniRef50_UPI00005A0143 Cluster: PREDICTED: similar to Calmodulin... 36 0.37
UniRef50_P02607 Cluster: Myosin light polypeptide 6; n=34; Eutel... 36 0.37
UniRef50_P27482 Cluster: Calmodulin-like protein 3; n=476; cellu... 36 0.37
UniRef50_A7RV39 Cluster: Predicted protein; n=2; Nematostella ve... 35 0.86
UniRef50_Q9D6P8 Cluster: Calmodulin-like protein 3; n=640; Eukar... 35 0.86
UniRef50_O45209 Cluster: Calmodulin-like protein; n=3; Eukaryota... 35 1.1
UniRef50_Q014Q8 Cluster: Calcineurin B regulatory subunit; n=4; ... 34 2.0
UniRef50_A2ETI7 Cluster: PIKK family atypical protein kinase; n=... 33 3.5
UniRef50_P53141 Cluster: Myosin light chain 1; n=6; Saccharomyce... 33 4.6
UniRef50_A5NW30 Cluster: PfkB domain protein; n=3; Alphaproteoba... 32 6.0
UniRef50_UPI0000E4A62F Cluster: PREDICTED: similar to calmodulin... 32 8.0
UniRef50_UPI00004987C9 Cluster: EF-hand calcium binding protein;... 32 8.0
>UniRef50_P06742 Cluster: Myosin light chain alkali; n=35;
Arthropoda|Rep: Myosin light chain alkali - Drosophila
melanogaster (Fruit fly)
Length = 155
Score = 69.7 bits (163), Expect = 3e-11
Identities = 30/50 (60%), Positives = 39/50 (78%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
ML AEL H LLALGE LDD +V + DCMDPEDD+G IPY+ F++++M+
Sbjct: 100 MLLAELQHALLALGESLDDEQVETLFADCMDPEDDEGFIPYSQFVQRLMS 149
>UniRef50_UPI0000D556DD Cluster: PREDICTED: similar to CG5596-PA,
isoform A; n=1; Tribolium castaneum|Rep: PREDICTED:
similar to CG5596-PA, isoform A - Tribolium castaneum
Length = 169
Score = 60.9 bits (141), Expect = 2e-08
Identities = 26/48 (54%), Positives = 37/48 (77%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 149
M EL+H+LL+LGEKL D+EV E+ +DC+D EDD+G I Y FL+++
Sbjct: 109 MAAGELSHSLLSLGEKLTDAEVDELFEDCLDEEDDEGDIEYIPFLRRM 156
>UniRef50_P53014 Cluster: Myosin, essential light chain; n=2;
Caenorhabditis|Rep: Myosin, essential light chain -
Caenorhabditis elegans
Length = 153
Score = 47.6 bits (108), Expect = 2e-04
Identities = 25/50 (50%), Positives = 32/50 (64%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
+L AEL H LLALGE+L E E+ K ED +GM+ Y F+KKV+A
Sbjct: 100 ILAAELRHILLALGERLSADEADELLKGV---EDGEGMVKYEDFIKKVLA 146
>UniRef50_P14649 Cluster: Myosin light polypeptide 6B; n=145;
Chordata|Rep: Myosin light polypeptide 6B - Homo sapiens
(Human)
Length = 208
Score = 42.3 bits (95), Expect = 0.006
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
++GAEL H L LGEK+ + EV V ED +G I Y AFLK +++
Sbjct: 161 VMGAELRHVLTTLGEKMTEEEVETV---LAGHEDSNGCINYEAFLKHILS 207
>UniRef50_P12829 Cluster: Myosin light polypeptide 4; n=63;
Euteleostomi|Rep: Myosin light polypeptide 4 - Homo
sapiens (Human)
Length = 197
Score = 42.3 bits (95), Expect = 0.006
Identities = 21/50 (42%), Positives = 32/50 (64%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
++GAEL H L LGEK+ ++EV ++ ED +G I Y AF+K +M+
Sbjct: 150 VMGAELRHVLATLGEKMTEAEVEQL---LAGQEDANGCINYEAFVKHIMS 196
>UniRef50_A0A9Q4 Cluster: Myosin light chain; n=5; Chordata|Rep:
Myosin light chain - Molgula tectiformis
Length = 153
Score = 41.9 bits (94), Expect = 0.007
Identities = 21/49 (42%), Positives = 30/49 (61%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 152
+LGAEL H L LGE++ + EV ++ ED +G I Y F+KKV+
Sbjct: 104 VLGAELRHVLATLGERMSEEEVDQLMS---GQEDANGCINYEVFIKKVL 149
>UniRef50_Q17133 Cluster: Myosin, essential light chain; n=3;
Metazoa|Rep: Myosin, essential light chain -
Branchiostoma floridae (Florida lancelet) (Amphioxus)
Length = 149
Score = 41.1 bits (92), Expect = 0.013
Identities = 21/50 (42%), Positives = 29/50 (58%)
Frame = +3
Query: 3 LMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 152
L+ GAEL H L LGEKL +++V E+ ED +G + Y F K +M
Sbjct: 98 LISGAELRHVLATLGEKLTEAQVDELMAGGGGQEDAEGNVNYDTFAKYLM 147
>UniRef50_P54357 Cluster: Myosin-2 essential light chain; n=23;
Eumetazoa|Rep: Myosin-2 essential light chain -
Drosophila melanogaster (Fruit fly)
Length = 147
Score = 38.3 bits (85), Expect = 0.092
Identities = 21/47 (44%), Positives = 27/47 (57%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
AEL H L LGEKL D EV ++ + ED G I Y F++ VM+
Sbjct: 103 AELRHLLTTLGEKLTDEEVEQLLANM---EDQQGNINYEEFVRMVMS 146
>UniRef50_P05945 Cluster: Myosin catalytic light chain LC-1, mantle
muscle; n=11; Bilateria|Rep: Myosin catalytic light
chain LC-1, mantle muscle - Todarodes pacificus
(Japanese flying squid)
Length = 160
Score = 37.9 bits (84), Expect = 0.12
Identities = 19/51 (37%), Positives = 29/51 (56%)
Frame = +3
Query: 3 LMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
L+ AE+ + L LGE++ + + ++ C ED DG I Y +KKVMA
Sbjct: 102 LISSAEIRNVLKMLGERITEDQCNDIFTFCDIREDIDGNIKYEDLMKKVMA 152
>UniRef50_UPI0000D9C1D1 Cluster: PREDICTED: similar to
Calmodulin-like protein 3 (Calmodulin-related protein
NB-1) (CaM-like protein) (CLP); n=1; Macaca mulatta|Rep:
PREDICTED: similar to Calmodulin-like protein 3
(Calmodulin-related protein NB-1) (CaM-like protein)
(CLP) - Macaca mulatta
Length = 175
Score = 36.3 bits (80), Expect = 0.37
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
AEL H + LGEKL D EV E+ + D DG + Y F++ +++
Sbjct: 130 AELRHVMTRLGEKLSDEEVDEMIRAA--DTDGDGQVNYEEFVRVLVS 174
>UniRef50_UPI00005A0143 Cluster: PREDICTED: similar to
Calmodulin-related protein NB-1 (Calmodulin-like
protein) (CLP); n=1; Canis lupus familiaris|Rep:
PREDICTED: similar to Calmodulin-related protein NB-1
(Calmodulin-like protein) (CLP) - Canis familiaris
Length = 409
Score = 36.3 bits (80), Expect = 0.37
Identities = 19/51 (37%), Positives = 28/51 (54%)
Frame = +3
Query: 3 LMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
L+ AEL H + LGEKL D EV E+ + D DG + Y F+ +++
Sbjct: 360 LVSAAELRHVMTRLGEKLSDEEVDEMIRAA--DVDGDGQVNYEEFVHMLVS 408
>UniRef50_P02607 Cluster: Myosin light polypeptide 6; n=34;
Euteleostomi|Rep: Myosin light polypeptide 6 - Gallus
gallus (Chicken)
Length = 151
Score = 36.3 bits (80), Expect = 0.37
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +3
Query: 6 MLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
++GAE+ H L+ LGEK+ + EV ++ ED +G I Y ++ V++
Sbjct: 104 VMGAEIRHVLVTLGEKMTEEEVEQLV---AGHEDSNGCINYEELVRMVLS 150
>UniRef50_P27482 Cluster: Calmodulin-like protein 3; n=476; cellular
organisms|Rep: Calmodulin-like protein 3 - Homo sapiens
(Human)
Length = 149
Score = 36.3 bits (80), Expect = 0.37
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
AEL H + LGEKL D EV E+ + D DG + Y F++ +++
Sbjct: 104 AELRHVMTRLGEKLSDEEVDEMIRAA--DTDGDGQVNYEEFVRVLVS 148
>UniRef50_A7RV39 Cluster: Predicted protein; n=2; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 151
Score = 35.1 bits (77), Expect = 0.86
Identities = 20/50 (40%), Positives = 29/50 (58%)
Frame = +3
Query: 3 LMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 152
L+ AEL H L +LG+KL D EV + + ED+ G + Y F++ VM
Sbjct: 103 LIGAAELRHLLASLGDKLSDEEVDNL---MVGFEDNQGQVFYEDFVRAVM 149
>UniRef50_Q9D6P8 Cluster: Calmodulin-like protein 3; n=640;
Eukaryota|Rep: Calmodulin-like protein 3 - Mus musculus
(Mouse)
Length = 149
Score = 35.1 bits (77), Expect = 0.86
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA 155
AEL H + LGEKL D EV E+ + D DG + Y F+ +++
Sbjct: 104 AELRHVMTKLGEKLSDEEVDEMIQAA--DTDGDGQVNYEEFVHMLVS 148
>UniRef50_O45209 Cluster: Calmodulin-like protein; n=3;
Eukaryota|Rep: Calmodulin-like protein - Branchiostoma
lanceolatum (Common lancelet) (Amphioxus)
Length = 225
Score = 34.7 bits (76), Expect = 1.1
Identities = 18/41 (43%), Positives = 23/41 (56%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 137
AEL H + LGEKL D EV E+ ++ D DG + Y F
Sbjct: 187 AELRHVMTNLGEKLTDEEVDEMIREA--DIDGDGQVNYEEF 225
>UniRef50_Q014Q8 Cluster: Calcineurin B regulatory subunit; n=4;
Ostreococcus|Rep: Calcineurin B regulatory subunit -
Ostreococcus tauri
Length = 1711
Score = 33.9 bits (74), Expect = 2.0
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 137
AE+ H L ++GEK D E+ E+ M D DG I Y F
Sbjct: 1398 AEIAHVLASMGEKATDDEIDEMI--LMADTDGDGQISYEEF 1436
>UniRef50_A2ETI7 Cluster: PIKK family atypical protein kinase; n=1;
Trichomonas vaginalis G3|Rep: PIKK family atypical
protein kinase - Trichomonas vaginalis G3
Length = 2228
Score = 33.1 bits (72), Expect = 3.5
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +2
Query: 338 IPYNILDTDKPLFLYEQHNINCFVQLSTFDCT 433
+PY +D PLFLY H+INC ++T C+
Sbjct: 864 LPY--IDQLMPLFLYHIHDINCLSVIATLSCS 893
>UniRef50_P53141 Cluster: Myosin light chain 1; n=6;
Saccharomycetales|Rep: Myosin light chain 1 -
Saccharomyces cerevisiae (Baker's yeast)
Length = 149
Score = 32.7 bits (71), Expect = 4.6
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +3
Query: 18 ELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 152
+L + L LGEKL D+EV E+ K D +G I Y F++ V+
Sbjct: 105 DLRYMLTGLGEKLTDAEVDELLKGV--EVDSNGEIDYKKFIEDVL 147
>UniRef50_A5NW30 Cluster: PfkB domain protein; n=3;
Alphaproteobacteria|Rep: PfkB domain protein -
Methylobacterium sp. 4-46
Length = 355
Score = 32.3 bits (70), Expect = 6.0
Identities = 20/49 (40%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Frame = -2
Query: 322 SAGWLRAPPEELSPPRALPA---PVPQSR-ASVF*GPSHRTFVAGKDLI 188
S GW PP L+PPR PA P+P R GP V G+ LI
Sbjct: 5 STGWRMRPPAPLAPPRRAPAPGRPLPACRPPGARSGPIPMILVCGEALI 53
>UniRef50_UPI0000E4A62F Cluster: PREDICTED: similar to calmodulin;
n=2; Strongylocentrotus purpuratus|Rep: PREDICTED:
similar to calmodulin - Strongylocentrotus purpuratus
Length = 283
Score = 31.9 bits (69), Expect = 8.0
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +3
Query: 15 AELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 149
AEL + + G L D EVA++ K+ ++ DG + Y FL+K+
Sbjct: 202 AELKTVMKSFGVPLTDKEVAKIFKEA--DKNGDGKVNYEEFLRKI 244
>UniRef50_UPI00004987C9 Cluster: EF-hand calcium binding protein;
n=1; Entamoeba histolytica HM-1:IMSS|Rep: EF-hand
calcium binding protein - Entamoeba histolytica
HM-1:IMSS
Length = 153
Score = 31.9 bits (69), Expect = 8.0
Identities = 19/48 (39%), Positives = 26/48 (54%)
Frame = +3
Query: 18 ELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVMA*K 161
ELT LL +GE+ SEV E+ E+ +G I Y F++ V A K
Sbjct: 109 ELTEALLNIGERCTTSEVGELKTVA---ENQEGQIRYELFIQAVFAKK 153
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 414,868,422
Number of Sequences: 1657284
Number of extensions: 7187003
Number of successful extensions: 25920
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 24107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25823
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 28437262108
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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