BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31349 (321 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 32 0.019 SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 31 0.043 SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc... 30 0.075 SPAC18G6.15 |mal3||EB1 family Mal3|Schizosaccharomyces pombe|chr... 29 0.13 SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr 2|||M... 28 0.30 SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 28 0.40 SPAC23H3.07c |mrp2||mitochondrial ribosomal protein subunit S14|... 27 0.70 SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 27 0.70 SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pomb... 27 0.93 SPAC2F7.07c |||histone deacetylase complex subunit Rco1 |Schizos... 27 0.93 SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 26 1.2 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 26 1.6 SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 25 2.8 SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe... 25 3.8 SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar... 25 3.8 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 3.8 SPBC14F5.08 |med7||mediator complex subunit Med7|Schizosaccharom... 24 5.0 SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyce... 24 5.0 SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 24 6.6 SPBC1347.10 |cdc23|mcm10|MCM-associated protein Mcm10|Schizosacc... 23 8.7 SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Sc... 23 8.7 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 32.3 bits (70), Expect = 0.019 Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 6/91 (6%) Frame = -3 Query: 265 QHEATLANLRKKHNDAVSEMGEQLDQLNKLKAKAEKER---AQYFSEVNDLRAGLDHLSN 95 Q L L K+ E + D+L L+ K++K+ A + ++ + L+ L+N Sbjct: 1092 QRNVELEELTSKYGKLGEENAQIKDELLALRKKSKKQHDLCANFVDDLKEKSDALEQLTN 1151 Query: 94 EKAAQEKIVKQLQHQLNE---VQSKADEANR 11 EK E IV Q N V+ ++D ANR Sbjct: 1152 EK--NELIVSLEQSNSNNEALVEERSDLANR 1180 Score = 25.8 bits (54), Expect = 1.6 Identities = 14/63 (22%), Positives = 30/63 (47%) Frame = -3 Query: 199 QLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQLQHQLNEVQSKADE 20 +L ++N + K+ + DLRA L+ SNE + ++ + +LN ++ + E Sbjct: 442 KLAEINSERDFQNKKIKDFEKIEQDLRACLNSSSNELKEKSALIDKKDQELNNLREQIKE 501 Query: 19 ANR 11 + Sbjct: 502 QKK 504 Score = 23.4 bits (48), Expect = 8.7 Identities = 17/77 (22%), Positives = 35/77 (45%) Frame = -3 Query: 232 KHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQLQH 53 K A+ + +Q +LN L+ + ++++ S + L++ + NEK E QL Sbjct: 479 KEKSALIDKKDQ--ELNNLREQIKEQKKVSESTQSSLQSLQRDILNEKKKHEVYESQLNE 536 Query: 52 QLNEVQSKADEANRSRS 2 E+Q++ + S Sbjct: 537 LKGELQTEISNSEHLSS 553 >SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1115 Score = 31.1 bits (67), Expect = 0.043 Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 7/73 (9%) Frame = -3 Query: 250 LANLRKKHNDAVSEMGEQLDQL-------NKLKAKAEKERAQYFSEVNDLRAGLDHLSNE 92 L+NL+++HND + + ++L + + LK++ E+ QY + LR D L N Sbjct: 724 LSNLKERHNDELDSLNKKLREFEGILSSNSSLKSEIEERNNQYVT----LRENFDSLQN- 778 Query: 91 KAAQEKIVKQLQH 53 A E KQ+ H Sbjct: 779 -AIMETFDKQVTH 790 >SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 503 Score = 30.3 bits (65), Expect = 0.075 Identities = 18/71 (25%), Positives = 36/71 (50%) Frame = -3 Query: 226 NDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQLQHQL 47 ++ V + ++++QLN + K E ++A S+ L + + K+ Q+K + Sbjct: 19 DEEVQRISDKVNQLNTSENKNEDQKATNLSD--RLGPKITENVDAKSEQDKATNTIAEDA 76 Query: 46 NEVQSKADEAN 14 N QS+ DE+N Sbjct: 77 NTKQSENDESN 87 >SPAC18G6.15 |mal3||EB1 family Mal3|Schizosaccharomyces pombe|chr 1|||Manual Length = 308 Score = 29.5 bits (63), Expect = 0.13 Identities = 17/68 (25%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = -3 Query: 253 TLANLRKKH-NDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLD-HLSNEKAAQ 80 T A LR K ++ + QL ++N+ E+ER YF+++ ++ + HL+ + Sbjct: 168 TAAVLRAKQAQQQITSLETQLYEVNETMFGLERERDFYFNKLREIEILVQTHLTTSPMSM 227 Query: 79 EKIVKQLQ 56 E +++++Q Sbjct: 228 ENMLERIQ 235 >SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 28.3 bits (60), Expect = 0.30 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = -3 Query: 253 TLANLRKKHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLD 107 ++ +R K + S + + + LNK K +K+ A+Y N L+ ++ Sbjct: 89 SITQMRTKFDKTKSWLSSKRNTLNKSKESLQKDEAEYVELANSLKTKVE 137 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 27.9 bits (59), Expect = 0.40 Identities = 18/65 (27%), Positives = 32/65 (49%) Frame = -3 Query: 238 RKKHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQL 59 RKK + +E QL ++N+ + KAEK Q + + L+ E+A E+ + Sbjct: 35 RKKLLNNKNEYLRQLSEINEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSF 94 Query: 58 QHQLN 44 Q +L+ Sbjct: 95 QEKLD 99 >SPAC23H3.07c |mrp2||mitochondrial ribosomal protein subunit S14|Schizosaccharomyces pombe|chr 1|||Manual Length = 105 Score = 27.1 bits (57), Expect = 0.70 Identities = 15/36 (41%), Positives = 20/36 (55%) Frame = +1 Query: 199 AHPSRRRHRCASCGGWRGWLRAGCWPPRGGARYPIR 306 AHP++ ++RC G RG RA G AR+P R Sbjct: 59 AHPTKIKNRCIETGRGRGVFRA-----FGLARFPFR 89 >SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 906 Score = 27.1 bits (57), Expect = 0.70 Identities = 21/63 (33%), Positives = 32/63 (50%) Frame = -3 Query: 280 EEANIQHEATLANLRKKHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHL 101 EEA+ +++ +L K+ E EQL K K EKE + S+ DL+ GL L Sbjct: 87 EEADKIYQSVEEHLSKRRKSQ-REKQEQLQ-----KEKYEKENPKVSSQFADLKRGLSTL 140 Query: 100 SNE 92 ++E Sbjct: 141 TDE 143 >SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 455 Score = 26.6 bits (56), Expect = 0.93 Identities = 15/66 (22%), Positives = 28/66 (42%) Frame = -3 Query: 286 PLEEANIQHEATLANLRKKHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLD 107 P+EEAN ++E+ A ++ S Q Q + E + +E+N + + Sbjct: 69 PIEEANEENESVAATSNERQQHDASNQPSQAAQ-TTINKNTESATPKTVNEINKSQEFQE 127 Query: 106 HLSNEK 89 H+ K Sbjct: 128 HVETPK 133 >SPAC2F7.07c |||histone deacetylase complex subunit Rco1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 26.6 bits (56), Expect = 0.93 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -3 Query: 289 PPLEEANIQHEATLANLRKKHNDAVSEMGEQLDQLNKLK 173 PP+EE N+ +A N KH+ +E+ EQ + + +K Sbjct: 292 PPIEEKNLPDDAWYCN-ECKHHSLYNELDEQEELESNVK 329 >SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosaccharomyces pombe|chr 2|||Manual Length = 421 Score = 26.2 bits (55), Expect = 1.2 Identities = 16/57 (28%), Positives = 28/57 (49%) Frame = -3 Query: 193 DQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQLQHQLNEVQSKAD 23 D L K A K+ A ++ + L NEK EK+++Q Q++ N+ + K + Sbjct: 43 DSLQKSFLDALKQSATDSELLHKNLDEIKFLQNEKLNNEKLLEQEQNEANDYRLKVE 99 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 25.8 bits (54), Expect = 1.6 Identities = 15/53 (28%), Positives = 24/53 (45%) Frame = -3 Query: 217 VSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKAAQEKIVKQL 59 VSE G+ D K+ E+ +S+ N++ D LSN + + V L Sbjct: 1026 VSERGDYDDNAKKVDEVISIEKVDGYSKENNVPLSEDDLSNSSESSNESVYSL 1078 >SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 25.0 bits (52), Expect = 2.8 Identities = 14/29 (48%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = -2 Query: 296 YRAPPRGGQHPARSHPR-QPPQEAQRCRL 213 YR PPR G+H R+HP P Q R L Sbjct: 369 YR-PPRKGKHNDRAHPPIHPVQMVHRSAL 396 >SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 24.6 bits (51), Expect = 3.8 Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 1/31 (3%) Frame = +3 Query: 228 CFLRR-LARVASCWMLASSRGGPVPNSPYSE 317 CFL+ LARV +L S RG + N P E Sbjct: 59 CFLKENLARVLVHDLLMSKRGLSISNGPIKE 89 >SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 24.6 bits (51), Expect = 3.8 Identities = 17/75 (22%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Frame = -3 Query: 259 EATLANLRK-KHNDAVSEMGEQL-DQLNKLKAKAEKERAQYFSEVNDLRAGLDHLSNEKA 86 E L L K +H + ++L DQ+ K K E + Q ++++ + L+H + + Sbjct: 296 EECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQLSNAQEKLEHEQHSRN 355 Query: 85 AQEKIVKQLQHQLNE 41 + + KQ + E Sbjct: 356 QKLEAAKQRMDNIRE 370 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 24.6 bits (51), Expect = 3.8 Identities = 17/76 (22%), Positives = 38/76 (50%), Gaps = 1/76 (1%) Frame = -3 Query: 235 KKHNDAVSEMGEQLDQLNKLKAKAEKERAQYFSEVNDLRAGLDHL-SNEKAAQEKIVKQL 59 +K N+++ L++ +L K +E ++ DLR LD+ SN K +E++ Sbjct: 634 EKLNESLKTSKTNLEEQTQLAEKYHEELLDNQQKLYDLRIELDYTKSNCKQMEEEMQVLR 693 Query: 58 QHQLNEVQSKADEANR 11 + +E++ +E ++ Sbjct: 694 EGHESEIKDFIEEHSK 709 >SPBC14F5.08 |med7||mediator complex subunit Med7|Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 24.2 bits (50), Expect = 5.0 Identities = 14/43 (32%), Positives = 21/43 (48%) Frame = -3 Query: 283 LEEANIQHEATLANLRKKHNDAVSEMGEQLDQLNKLKAKAEKE 155 L E ++HE + L + HN +M E L++ L EKE Sbjct: 286 LLEKQLKHEESQVELLRTHN---RQMTETLEKYKSLDFNMEKE 325 >SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyces pombe|chr 3|||Manual Length = 520 Score = 24.2 bits (50), Expect = 5.0 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = -3 Query: 145 YFSEVNDLRAGLDHLSNEKAAQEKIVKQLQHQLNEVQSK 29 Y E N + LSN + Q K V++ Q Q NE+ K Sbjct: 212 YHPEANQIIKRALLLSNGLSVQPKSVEESQIQFNELAQK 250 >SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase Gpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1448 Score = 23.8 bits (49), Expect = 6.6 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = -1 Query: 150 LNTLAKSMTSVPVSTTCPTK 91 L++ K++ S P+ST+ PTK Sbjct: 843 LSSYLKTLESTPMSTSSPTK 862 >SPBC1347.10 |cdc23|mcm10|MCM-associated protein Mcm10|Schizosaccharomyces pombe|chr 2|||Manual Length = 593 Score = 23.4 bits (48), Expect = 8.7 Identities = 9/13 (69%), Positives = 11/13 (84%) Frame = -3 Query: 67 KQLQHQLNEVQSK 29 K+LQ QLNE+Q K Sbjct: 17 KRLQRQLNEIQEK 29 >SPAC1556.06.1 |meu1|SPAC1556.06a, SPAC1556.06|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 776 Score = 23.4 bits (48), Expect = 8.7 Identities = 17/80 (21%), Positives = 37/80 (46%), Gaps = 3/80 (3%) Frame = -3 Query: 283 LEEANIQHEATLANLRKKHNDAVSEMGEQLDQLNKLKA---KAEKERAQYFSEVNDLRAG 113 L+E++ + + L+++ A+ + + + +K+ K E E +EV D+R Sbjct: 225 LKESDSFKDHEILRLKEERTAAMQAIDDISGTIETIKSDCYKVESENKGLINEVMDMRNF 284 Query: 112 LDHLSNEKAAQEKIVKQLQH 53 + L E A E ++Q+ Sbjct: 285 VQQLEQELYAFEDDYSRIQN 304 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 956,236 Number of Sequences: 5004 Number of extensions: 11951 Number of successful extensions: 86 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 81 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 86 length of database: 2,362,478 effective HSP length: 63 effective length of database: 2,047,226 effective search space used: 88030718 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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