BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31342 (771 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 212 3e-55 At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 99 3e-21 At5g63910.1 68418.m08025 expressed protein 33 0.28 At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera... 31 1.1 At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera... 31 1.1 At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy... 29 2.6 At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 3.4 At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr... 29 3.4 At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-depende... 29 3.4 At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit... 28 7.9 >At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+] / GPDH strong similarity to SP|P52425 Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8) {Cuphea lanceolata}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 400 Score = 212 bits (517), Expect = 3e-55 Identities = 107/218 (49%), Positives = 142/218 (65%), Gaps = 2/218 (0%) Frame = +1 Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENV 294 K+KV +VGSGNWGS AK++ NA L +F D V MWV+EE++ G+KL ++IN+T+ENV Sbjct: 54 KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENV 113 Query: 295 KYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKG 474 KYLPG KL NVVA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG Sbjct: 114 KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKG 173 Query: 475 FDIAEGGGIDLISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRD 651 ++ + G +IS +I++ L I C VLMGANIA+E+A EKF E T+G R +A Sbjct: 174 MEVKKEGPC-MISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQ 232 Query: 652 IIQTDYFRXXXXXXXXXXXICGALKNIVAVVPGSWDGL 765 + T YF +CG LKN+VA+ G DGL Sbjct: 233 LFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGL 270 >At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehydrogenase family protein low similarity to SP|Q26756 Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 420 Score = 99.1 bits (236), Expect = 3e-21 Identities = 67/217 (30%), Positives = 100/217 (46%), Gaps = 3/217 (1%) Frame = +1 Query: 124 KVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVKYL 303 KV ++G G++G+A+A V R L V M V + + + INE H N KY Sbjct: 89 KVVVLGGGSFGTAMAAHVARRKEGLE-----VNMLVRDSFV-----CQSINENHHNCKYF 138 Query: 304 PGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDI 483 P HKLP NV+A D A DAD + VP QF + + + P +SL KG ++ Sbjct: 139 PEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLEL 198 Query: 484 AEGGGIDLISHIITRCLK---IPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDI 654 + ++S II LK P L G + A E+ + +D LA ++ + Sbjct: 199 ---NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQL 255 Query: 655 IQTDYFRXXXXXXXXXXXICGALKNIVAVVPGSWDGL 765 + + Y R I GALKN++A+ G DG+ Sbjct: 256 LASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGM 292 >At5g63910.1 68418.m08025 expressed protein Length = 500 Score = 32.7 bits (71), Expect = 0.28 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%) Frame = +1 Query: 127 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT-EIINETHE 288 VCIVGSG GS++A + + S + ++ M+ EI+ G+ T + +T E Sbjct: 38 VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRTVTVAGDTFE 92 >At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 481 Score = 30.7 bits (66), Expect = 1.1 Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 5/80 (6%) Frame = +1 Query: 82 DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 261 D +LD ++QP V + G+ G AK + A L + R +WV ++G Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308 Query: 262 TEIINE----THENV-KYLP 306 +E ++ T +N +YLP Sbjct: 309 SEYVSANGGGTEDNTPEYLP 328 >At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 487 Score = 30.7 bits (66), Expect = 1.1 Identities = 20/82 (24%), Positives = 35/82 (42%) Frame = +1 Query: 91 ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEI 270 +LD +KQP V + G+ GS AK + A L + R +WV ++G + Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDGSACSAY 316 Query: 271 INETHENVKYLPGHKLPSNVVA 336 ++ ++ LP V+ Sbjct: 317 LSANSGKIRDGTPDYLPEGFVS 338 >At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehydrogenase family protein weak similarity to SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile PF01210: NAD-dependent glycerol-3-phosphate dehydrogenase Length = 455 Score = 29.5 bits (63), Expect = 2.6 Identities = 35/146 (23%), Positives = 62/146 (42%), Gaps = 8/146 (5%) Frame = +1 Query: 337 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGGIDL 507 V ++ EA DAD++I +P + + + K A +SL KG + + Sbjct: 141 VTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEFEPHPRI 200 Query: 508 IS--HIITRCLKIPCAVLM---GANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 672 ++ +I R IP ++ G NIASEV +++ I + PL + ++ +F Sbjct: 201 VTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGK-FLRQSHF 259 Query: 673 RXXXXXXXXXXXICGALKNIVAVVPG 750 + G LKN+ A+ G Sbjct: 260 IVWDNSDLITHEVMGGLKNVYAIGAG 285 >At5g51490.1 68418.m06386 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 536 Score = 29.1 bits (62), Expect = 3.4 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +1 Query: 508 ISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMR 648 ISH+I+ CL + A+L N + A +K T + +D L +R Sbjct: 173 ISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAVR 219 >At2g29770.1 68415.m03617 kelch repeat-containing F-box family protein contains Pfam PF00646: F-box domain; contains Pfam PF01344 : Kelch motif; similar to SKP1 interacting partner 6 (GI:10716957) [Arabidopsis thaliana] Length = 387 Score = 29.1 bits (62), Expect = 3.4 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +3 Query: 552 INGSQYCIGGC*GKILRNDHWL 617 I+G Y IGGC + RNDHW+ Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199 >At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-dependent thioredoxin reductase 2 (NTR2) identical to SP|Q39242 Length = 330 Score = 29.1 bits (62), Expect = 3.4 Identities = 26/79 (32%), Positives = 35/79 (44%) Frame = +1 Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVK 297 K KVCIVGSG A R FE W+ +I G +LT T +V+ Sbjct: 5 KTKVCIVGSGPAAHTAAIYASRAELKPLLFEG----WMANDIAPGGQLT-----TTTDVE 55 Query: 298 YLPGHKLPSNVVAVPDVVE 354 PG P ++ + D+VE Sbjct: 56 NFPG--FPEGILGI-DIVE 71 >At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity to SP|Q10567 Adapter-related protein complex 1 beta 1 subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin [Drosophila melanogaster] GI:434902; contains Pfam profile: PF01602 Adaptin N terminal region Length = 893 Score = 27.9 bits (59), Expect = 7.9 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -2 Query: 638 GASITSRQPMVVSQNFSSATSDAIL 564 GAS + PMV+SQN S+ ++ ++L Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,433,324 Number of Sequences: 28952 Number of extensions: 302302 Number of successful extensions: 931 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 898 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 926 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1716774400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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