SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31342
         (771 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD...   212   3e-55
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy...    99   3e-21
At5g63910.1 68418.m08025 expressed protein                             33   0.28 
At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera...    31   1.1  
At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera...    31   1.1  
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy...    29   2.6  
At5g51490.1 68418.m06386 pectinesterase family protein contains ...    29   3.4  
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr...    29   3.4  
At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-depende...    29   3.4  
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit...    28   7.9  

>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
           / GPDH strong similarity to  SP|P52425
           Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
           {Cuphea lanceolata}; contains Pfam profile PF01210:
           NAD-dependent glycerol-3-phosphate dehydrogenase
          Length = 400

 Score =  212 bits (517), Expect = 3e-55
 Identities = 107/218 (49%), Positives = 142/218 (65%), Gaps = 2/218 (0%)
 Frame = +1

Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINETHENV 294
           K+KV +VGSGNWGS  AK++  NA  L +F D V MWV+EE++  G+KL ++IN+T+ENV
Sbjct: 54  KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINKTNENV 113

Query: 295 KYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKG 474
           KYLPG KL  NVVA PD+  A KDA++L+FV PHQF+  IC  L GKI     A+SL+KG
Sbjct: 114 KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKG 173

Query: 475 FDIAEGGGIDLISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRD 651
            ++ + G   +IS +I++ L I C VLMGANIA+E+A EKF E T+G R    +A     
Sbjct: 174 MEVKKEGPC-MISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQ 232

Query: 652 IIQTDYFRXXXXXXXXXXXICGALKNIVAVVPGSWDGL 765
           +  T YF            +CG LKN+VA+  G  DGL
Sbjct: 233 LFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGL 270


>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein low similarity to SP|Q26756
           Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
           (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
           Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 420

 Score = 99.1 bits (236), Expect = 3e-21
 Identities = 67/217 (30%), Positives = 100/217 (46%), Gaps = 3/217 (1%)
 Frame = +1

Query: 124 KVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVKYL 303
           KV ++G G++G+A+A  V R    L      V M V +  +      + INE H N KY 
Sbjct: 89  KVVVLGGGSFGTAMAAHVARRKEGLE-----VNMLVRDSFV-----CQSINENHHNCKYF 138

Query: 304 PGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDI 483
           P HKLP NV+A  D   A  DAD  +  VP QF  +    +   + P    +SL KG ++
Sbjct: 139 PEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLEL 198

Query: 484 AEGGGIDLISHIITRCLK---IPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDI 654
                + ++S II   LK    P   L G + A E+         +  +D  LA  ++ +
Sbjct: 199 ---NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQL 255

Query: 655 IQTDYFRXXXXXXXXXXXICGALKNIVAVVPGSWDGL 765
           + + Y R           I GALKN++A+  G  DG+
Sbjct: 256 LASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGM 292


>At5g63910.1 68418.m08025 expressed protein
          Length = 500

 Score = 32.7 bits (71), Expect = 0.28
 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
 Frame = +1

Query: 127 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT-EIINETHE 288
           VCIVGSG  GS++A  +   + S    + ++ M+   EI+ G+  T  +  +T E
Sbjct: 38  VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRTVTVAGDTFE 92


>At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 481

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 22/80 (27%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
 Frame = +1

Query: 82  DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 261
           D  +LD  ++QP   V  +  G+ G   AK +   A  L   + R  +WV    ++G   
Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308

Query: 262 TEIINE----THENV-KYLP 306
           +E ++     T +N  +YLP
Sbjct: 309 SEYVSANGGGTEDNTPEYLP 328


>At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 487

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 20/82 (24%), Positives = 35/82 (42%)
 Frame = +1

Query: 91  ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEI 270
           +LD  +KQP   V  +  G+ GS  AK +   A  L   + R  +WV    ++G   +  
Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDGSACSAY 316

Query: 271 INETHENVKYLPGHKLPSNVVA 336
           ++     ++      LP   V+
Sbjct: 317 LSANSGKIRDGTPDYLPEGFVS 338


>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 455

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 35/146 (23%), Positives = 62/146 (42%), Gaps = 8/146 (5%)
 Frame = +1

Query: 337 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGGIDL 507
           V ++ EA  DAD++I  +P      + + +    K    A   +SL KG +        +
Sbjct: 141 VTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEFEPHPRI 200

Query: 508 IS--HIITRCLKIPCAVLM---GANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 672
           ++   +I R   IP   ++   G NIASEV  +++    I   +    PL +  ++  +F
Sbjct: 201 VTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGK-FLRQSHF 259

Query: 673 RXXXXXXXXXXXICGALKNIVAVVPG 750
                       + G LKN+ A+  G
Sbjct: 260 IVWDNSDLITHEVMGGLKNVYAIGAG 285


>At5g51490.1 68418.m06386 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 536

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = +1

Query: 508 ISHIITRCLKIPCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMR 648
           ISH+I+ CL +  A+L   N  +  A +K   T +  +D  L   +R
Sbjct: 173 ISHLISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAVR 219


>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
           protein contains Pfam PF00646: F-box domain; contains
           Pfam PF01344 : Kelch motif; similar to SKP1 interacting
           partner 6 (GI:10716957) [Arabidopsis thaliana]
          Length = 387

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +3

Query: 552 INGSQYCIGGC*GKILRNDHWL 617
           I+G  Y IGGC   + RNDHW+
Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199


>At2g17420.1 68415.m02010 thioredoxin reductase 2 / NADPH-dependent
           thioredoxin reductase 2 (NTR2) identical to SP|Q39242
          Length = 330

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 26/79 (32%), Positives = 35/79 (44%)
 Frame = +1

Query: 118 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLTEIINETHENVK 297
           K KVCIVGSG      A    R       FE     W+  +I  G +LT     T  +V+
Sbjct: 5   KTKVCIVGSGPAAHTAAIYASRAELKPLLFEG----WMANDIAPGGQLT-----TTTDVE 55

Query: 298 YLPGHKLPSNVVAVPDVVE 354
             PG   P  ++ + D+VE
Sbjct: 56  NFPG--FPEGILGI-DIVE 71


>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
           to SP|Q10567 Adapter-related protein complex 1 beta 1
           subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
           [Drosophila melanogaster] GI:434902; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 893

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -2

Query: 638 GASITSRQPMVVSQNFSSATSDAIL 564
           GAS  +  PMV+SQN S+ ++ ++L
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,433,324
Number of Sequences: 28952
Number of extensions: 302302
Number of successful extensions: 931
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -