Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31333
(591 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 31 0.57
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 30 1.3
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 30 1.3
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 3.1
At2g05990.2 68415.m00652 enoyl-[acyl-carrier protein] reductase ... 29 3.1
At2g05990.1 68415.m00651 enoyl-[acyl-carrier protein] reductase ... 29 3.1
At1g72110.1 68414.m08335 expressed protein 29 3.1
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 4.1
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 28 4.1
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik... 28 5.4
At5g55700.1 68418.m06944 glycosyl hydrolase family 14 protein si... 27 7.1
At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to a... 27 7.1
At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to e... 27 7.1
At3g13780.1 68416.m01739 expressed protein 27 7.1
At1g26930.1 68414.m03283 kelch repeat-containing F-box family pr... 27 7.1
At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein 27 9.4
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 27 9.4
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 31.1 bits (67), Expect = 0.57
Identities = 22/61 (36%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIARALAYIAKKHSSQEVSDK 568
S+S TSP P S Y Y PT + PTSP Q A+ IA S+ +S
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARLSPA 1750
Query: 569 S 571
S
Sbjct: 1751 S 1751
Score = 29.1 bits (62), Expect = 2.3
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +2
Query: 305 TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 478
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 479 I-PTSP 493
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 28.7 bits (61), Expect = 3.1
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +2
Query: 281 GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 460
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 461 QPTGNAI-PTSP 493
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
Score = 27.5 bits (58), Expect = 7.1
Identities = 27/97 (27%), Positives = 39/97 (40%), Gaps = 5/97 (5%)
Frame = +2
Query: 218 PKVSEGYGAETVKFGNEINPDGSYTY--FYETNNGIAAQEQGVPRNLGGNPPAVPVVA-- 385
P S GY + + + +P S T + T+ G + + G P P +
Sbjct: 1539 PSSSPGYSPSSPGY-SPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPT 1597
Query: 386 QGSFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1598 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634
Score = 27.1 bits (57), Expect = 9.4
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1607 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1641
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S Y Y PT A PTSP
Sbjct: 1628 SYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSP 1662
Score = 27.1 bits (57), Expect = 9.4
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 392 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 493
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1663 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1697
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.3
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 221 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 391
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.3
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 221 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 391
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 3.1
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -2
Query: 422 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 321
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At2g05990.2 68415.m00652 enoyl-[acyl-carrier protein] reductase
[NADH], chloroplast, putative / NADH-dependent enoyl-ACP
reductase, putative strong similarity to
enoyl-[acyl-carrier protein] reductase [NADH] SP:P80030
from [Brassica napus]
Length = 390
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = +2
Query: 407 SPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARALAYIAKKHSSQEVS 562
+P G IS+ Y+A E G + ++ E R LAY A + S+ V+
Sbjct: 254 NPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKSNIRVN 305
>At2g05990.1 68415.m00651 enoyl-[acyl-carrier protein] reductase
[NADH], chloroplast, putative / NADH-dependent enoyl-ACP
reductase, putative strong similarity to
enoyl-[acyl-carrier protein] reductase [NADH] SP:P80030
from [Brassica napus]
Length = 390
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = +2
Query: 407 SPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARALAYIAKKHSSQEVS 562
+P G IS+ Y+A E G + ++ E R LAY A + S+ V+
Sbjct: 254 NPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKSNIRVN 305
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 28.7 bits (61), Expect = 3.1
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +2
Query: 236 YGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 352
+G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3
Query: 345 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 455
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 28.3 bits (60), Expect = 4.1
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -3
Query: 487 SGDSITSGLVSVLVGDVIDADGNTFR 410
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
protein, putative similar to cold-regulated gene SRC2
[Glycine max] GI:2055230; contains Pfam profile PF00168:
C2 domain; identical to cDNA src2-like protein
GI:3426059
Length = 324
Score = 27.9 bits (59), Expect = 5.4
Identities = 24/79 (30%), Positives = 29/79 (36%), Gaps = 6/79 (7%)
Frame = +2
Query: 326 QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTGNAIPT 487
Q+ G P GG PP QG + P+ G P Y + GY P G P
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG---PY 260
Query: 488 SPPVPEQIARALAYIAKKH 544
P+Q A KKH
Sbjct: 261 G--YPQQQAHGKPQKPKKH 277
>At5g55700.1 68418.m06944 glycosyl hydrolase family 14 protein
similar to beta-amylase enzyme GI:6065749 from
[Arabidopsis thaliana]; contains Pfam profile PF01373:
Glycosyl hydrolase family 14
Length = 484
Score = 27.5 bits (58), Expect = 7.1
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = -2
Query: 584 KYVCEIYRLLLERNVSWRCRQEHERSAQALVGKWG*HYQW 465
K +C +L + R RQE E+S+ LV K G Y W
Sbjct: 309 KLICHADAILAKAADVLRRRQEEEKSSVMLVAKIGGIYWW 348
>At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to
alpha-expansin 4 precursor GI:16923355 from [Cucumis
sativus]; alpha-expansin gene family, PMID:11641069
Length = 260
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 191 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 84
++GW + W + T G+ TG G S R+ G+
Sbjct: 201 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 236
>At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to
expansin2 GI:4884433 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 259
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 191 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 84
++GW + W + T G+ TG G S R+ G+
Sbjct: 200 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 235
>At3g13780.1 68416.m01739 expressed protein
Length = 309
Score = 27.5 bits (58), Expect = 7.1
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 211 SDGGNRGDRDGQKRSRSGHGNER 143
++G + GD DG K+ R G G +R
Sbjct: 177 TNGNSDGDEDGDKKGRKGLGKKR 199
>At1g26930.1 68414.m03283 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 421
Score = 27.5 bits (58), Expect = 7.1
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +2
Query: 209 RVVPKVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGN 361
R K S G + + FG E++ Y Y TN+ A+ +PR L G+
Sbjct: 157 RYADKESLAVGTDLLVFGWEVSSYVIYRYSLLTNSWSTAKSMNMPRCLFGS 207
>At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein
Length = 774
Score = 27.1 bits (57), Expect = 9.4
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +2
Query: 344 RNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARAL 523
RNL N AVP + G + W S +++ ++D G A P + P+ I A
Sbjct: 74 RNLVNNAAAVPRLGIGGYEWWSE-----ALHGISDVGPGAKFGGAFPGATSFPQVITTAA 128
Query: 524 AY 529
++
Sbjct: 129 SF 130
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 27.1 bits (57), Expect = 9.4
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +1
Query: 79 RPSPV*K*ILQEEPIPVQHIPAVRSRDHSYSVSAHPDLLYCPRQSS 216
RPSP+ ++EE P+ ++P +RS + S S LY P SS
Sbjct: 67 RPSPMRGLPMEEETKPIVYLPRIRSEVFASSPSLLN--LYSPSSSS 110
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,150,089
Number of Sequences: 28952
Number of extensions: 300683
Number of successful extensions: 1040
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 982
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1029
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1171109464
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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