Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31308
(811 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 70 3e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 1e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.19
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.3
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 7.3
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 26 7.3
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 9.6
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 2e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +2
Query: 74 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 253
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 254 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 433
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 434 DDGMIPYAAFLKKVMA 481
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.1 bits (164), Expect = 3e-13
Identities = 43/135 (31%), Positives = 69/135 (51%), Gaps = 1/135 (0%)
Frame = +2
Query: 74 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 250
AFS++D + G I + LG ++R+L +PT A + FL + ++
Sbjct: 17 AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR 76
Query: 251 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 430
KD D E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 77 KMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 431 DDDGMIPYAAFLKKV 475
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 29.1 bits (62), Expect = 0.78
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +2
Query: 284 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 463
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 464 L 466
L
Sbjct: 71 L 71
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 1e-08
Identities = 35/147 (23%), Positives = 72/147 (48%)
Frame = +2
Query: 41 SARNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXX 220
+++ + AF +YD + G I ++G +LR+L N T A +
Sbjct: 3 ASKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKK 62
Query: 221 XXXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVA 400
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 63 FMSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQ 117
Query: 401 EVTKDCMDPEDDDGMIPYAAFLKKVMA 481
+ ++ DP + G Y F++++MA
Sbjct: 118 LMVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.1 bits (67), Expect = 0.19
Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
Frame = +2
Query: 74 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 250
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 251 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 430
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 431 DDDGMIPYAAFLKKVM 478
D DG I F+ +M
Sbjct: 158 DQDGEINEQEFIAIMM 173
Score = 29.5 bits (63), Expect = 0.59
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +2
Query: 242 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 415
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +2
Query: 47 RNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 157
R+ +E AF ++D + GKI NL + + LN N
Sbjct: 106 RDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 25.8 bits (54), Expect = 7.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +2
Query: 662 DIPYNILNTDKPLFLYEQHNINWFVQLSTFDCTY*SKINVL*TQKK 799
DIP N++ + +Y +N + +L T+ SK+N+ T K+
Sbjct: 714 DIPNKFQNSESRIIVYYSNNTLYLSELWLPQRTFSSKLNLAATPKR 759
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 7.3
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +2
Query: 368 LGEKLDDSEVAEVTKDCMDPED 433
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.8 bits (54), Expect = 7.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 47 RNDVERASFAFSIYDFEGKGKID 115
R+ + SF F +YDF G G +D
Sbjct: 611 RDVMRNISFIFELYDFNGDGFMD 633
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 9.6
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 51 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 224
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 225 KSSFPSTAKQRKTKT 269
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,052,626
Number of Sequences: 5004
Number of extensions: 57668
Number of successful extensions: 183
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -