BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31295 (757 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 28 1.3 SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe... 27 2.9 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 26 5.0 SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 26 6.7 SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 26 6.7 SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 8.8 SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 25 8.8 SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 8.8 >SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 28.3 bits (60), Expect = 1.3 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 3/30 (10%) Frame = +3 Query: 597 KTVYVGQ---NRPISESLNIAKTAPTDSGY 677 KTVYVG N PI E L+ +T P +S + Sbjct: 115 KTVYVGNLPPNTPIDEILSCVRTGPIESAW 144 >SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 548 Score = 27.1 bits (57), Expect = 2.9 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = +2 Query: 497 WIRSWHNGLGWRRWIS 544 W SW NG+ + +WIS Sbjct: 434 WRHSWSNGIDYAKWIS 449 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 26.2 bits (55), Expect = 5.0 Identities = 8/26 (30%), Positives = 11/26 (42%) Frame = +2 Query: 446 PLPNSQWTVS*RKEVKRWIRSWHNGL 523 PLP QW W+ +WH + Sbjct: 374 PLPGHQWAEVKHDNTVTWLATWHENI 399 >SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 25.8 bits (54), Expect = 6.7 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -1 Query: 541 NPSAPTQPVVPTTDPSFNL 485 +PS P + VV TT+P+F L Sbjct: 619 HPSVPVKLVVSTTEPAFQL 637 >SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 618 NRPISESLNIAKTAPTDSGYGVTR 689 NRP SLNI +T PT +G T+ Sbjct: 317 NRPGFPSLNIPRTRPTTAGIANTQ 340 >SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 25.4 bits (53), Expect = 8.8 Identities = 12/38 (31%), Positives = 23/38 (60%), Gaps = 2/38 (5%) Frame = -2 Query: 573 GRQLCN--LVSVEIHLRQPSPLCQLRIHLLTSFLYDTV 466 GR L + + +++HL QPS L Q + +T ++ +T+ Sbjct: 145 GRDLSDRPICYIQVHLHQPSKLTQNSLREMTVWVMETM 182 >SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 461 Score = 25.4 bits (53), Expect = 8.8 Identities = 9/32 (28%), Positives = 19/32 (59%) Frame = +3 Query: 615 QNRPISESLNIAKTAPTDSGYGVTRAPAVQRN 710 +NRP+ + +NI T ++ +G+T+ + N Sbjct: 301 KNRPLKDRINIVITRNSNYNFGLTKKEKMPEN 332 >SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 8.8 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -2 Query: 549 SVEIHLRQPSPLCQLRIHLLTSFLYD 472 S+ +HLR S L IHL S+ Y+ Sbjct: 152 SISLHLRSESAPISLPIHLYKSYSYN 177 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,185,823 Number of Sequences: 5004 Number of extensions: 69762 Number of successful extensions: 300 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 300 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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