Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31287
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 2.7
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 29 2.7
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 29 2.7
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 3.6
At1g72110.1 68414.m08335 expressed protein 29 3.6
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 4.8
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 28 4.8
At1g80870.1 68414.m09489 protein kinase family protein contains ... 28 6.3
At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to a... 27 8.4
At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to e... 27 8.4
At3g13780.1 68416.m01739 expressed protein 27 8.4
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik... 27 8.4
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.1 bits (62), Expect = 2.7
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +1
Query: 295 TNHGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 468
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 469 I-PTSP 483
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 29.1 bits (62), Expect = 2.7
Identities = 21/57 (36%), Positives = 26/57 (45%), Gaps = 4/57 (7%)
Frame = +1
Query: 382 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIAR---ALAYIAKNIPL 540
S+S TSP P S Y Y PT + PTSP Q A+ ++AY N L
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARL 1747
Score = 28.7 bits (61), Expect = 3.6
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +1
Query: 271 GSYTYFYETNHGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 450
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 451 QPTGNAI-PTSP 483
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 382 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 483
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 382 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 483
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 382 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 483
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 382 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 483
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
Score = 27.5 bits (58), Expect = 8.4
Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 8/71 (11%)
Frame = +2
Query: 365 PSLHRAHSPGHLLKVFP------SASITSPTRTDTSPLVMLSPLPHQCLSRSLVL-LPTS 523
PSLH+ GH +++ P + S+TSP D M +P +R+ VL L
Sbjct: 463 PSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMV 522
Query: 524 PRTFLS-RSNR 553
P+ +S ++NR
Sbjct: 523 PKCIVSPQANR 533
Score = 27.5 bits (58), Expect = 8.4
Identities = 27/97 (27%), Positives = 39/97 (40%), Gaps = 5/97 (5%)
Frame = +1
Query: 208 PKVSEGYGAETVKFGNEINPDGSYTY--FYETNHGIAAQEQGVPRNLGGNPPAVPVVA-- 375
P S GY + + + +P S T + T+ G + + G P P +
Sbjct: 1539 PSSSPGYSPSSPGY-SPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPT 1597
Query: 376 QGSFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 483
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1598 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.1 bits (62), Expect = 2.7
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +1
Query: 211 KVSEGYGAETVKFGNEINPDGSYTYFYETNHGIAAQEQGVPRNLGGNPP--AVPVVAQG 381
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.1 bits (62), Expect = 2.7
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +1
Query: 211 KVSEGYGAETVKFGNEINPDGSYTYFYETNHGIAAQEQGVPRNLGGNPP--AVPVVAQG 381
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -3
Query: 412 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 311
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +1
Query: 226 YGAETVKFGNEINPDGSYTYFYETNHGIAAQEQGVPRNL 342
+G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +2
Query: 335 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 445
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 28.3 bits (60), Expect = 4.8
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -1
Query: 477 SGDSITSGLVSVLVGDVIDADGNTFR 400
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At1g80870.1 68414.m09489 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 692
Score = 27.9 bits (59), Expect = 6.3
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 4/46 (8%)
Frame = -1
Query: 546 LLERNVLGDVGKSTSDLLRHWW----GSGDSITSGLVSVLVGDVID 421
LL N + D+GK + R WW GSG+ + V +G ID
Sbjct: 330 LLSPNSVLDLGKGSKQWGRDWWWKQEGSGELCSKDYVREWIGSQID 375
>At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to
alpha-expansin 4 precursor GI:16923355 from [Cucumis
sativus]; alpha-expansin gene family, PMID:11641069
Length = 260
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 181 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 74
++GW + W + T G+ TG G S R+ G+
Sbjct: 201 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 236
>At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to
expansin2 GI:4884433 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 259
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 181 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 74
++GW + W + T G+ TG G S R+ G+
Sbjct: 200 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 235
>At3g13780.1 68416.m01739 expressed protein
Length = 309
Score = 27.5 bits (58), Expect = 8.4
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 201 SDGGNRGDRDGQKRSRSGHGNER 133
++G + GD DG K+ R G G +R
Sbjct: 177 TNGNSDGDEDGDKKGRKGLGKKR 199
>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
protein, putative similar to cold-regulated gene SRC2
[Glycine max] GI:2055230; contains Pfam profile PF00168:
C2 domain; identical to cDNA src2-like protein
GI:3426059
Length = 324
Score = 27.5 bits (58), Expect = 8.4
Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 6/55 (10%)
Frame = +1
Query: 316 QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTG 462
Q+ G P GG PP QG + P+ G P Y + GY P G
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG 258
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,775,031
Number of Sequences: 28952
Number of extensions: 342040
Number of successful extensions: 1114
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1100
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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