Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31282
(622 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 201 3e-52
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 194 4e-50
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 190 8e-49
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 1.9
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.5
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.3
At3g05830.1 68416.m00654 expressed protein 29 3.3
At3g12240.1 68416.m01527 serine carboxypeptidase S10 family prot... 28 4.3
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 28 5.7
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 5.7
At3g49180.1 68416.m05375 transducin family protein / WD-40 repea... 28 5.7
At3g06490.1 68416.m00753 myb family transcription factor (MYB108... 28 5.7
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 5.7
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 7.6
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 7.6
At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 7.6
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 201 bits (490), Expect = 3e-52
Identities = 87/150 (58%), Positives = 117/150 (78%), Gaps = 1/150 (0%)
Frame = +2
Query: 134 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 313
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 314 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 490
IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L
Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123
Query: 491 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 580
Q+++ FD GK+L+ +V+ + GEE + A+K
Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEEQINALK 153
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 194 bits (473), Expect = 4e-50
Identities = 85/150 (56%), Positives = 115/150 (76%), Gaps = 1/150 (0%)
Frame = +2
Query: 134 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 313
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 314 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 490
IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L
Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123
Query: 491 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 580
TQL+ F+ GK+++ +V+ + GEE + A+K
Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEEQMCALK 153
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 190 bits (462), Expect = 8e-49
Identities = 83/150 (55%), Positives = 111/150 (74%), Gaps = 1/150 (0%)
Frame = +2
Query: 134 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 313
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 314 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 490
IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L
Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123
Query: 491 TQLRTDFDSGKELLCTVLKSCGEECVIAVK 580
Q+R FD GK+++ +V+ S GEE + AVK
Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEEQICAVK 153
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.5 bits (63), Expect = 1.9
Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = -1
Query: 544 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQVTIV-SHQGQVAIIRDISQLVVFALHVGY 368
+HGA + AA++V L +RD E +V V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 367 VHVVCGGTD 341
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.5
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +2
Query: 464 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 565
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.3
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -2
Query: 234 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 127
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g05830.1 68416.m00654 expressed protein
Length = 336
Score = 28.7 bits (61), Expect = 3.3
Identities = 24/82 (29%), Positives = 39/82 (47%)
Frame = +2
Query: 287 AKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDL 466
AK + G+D K ED+ P V+ +D ++T + D+ + LM +G +
Sbjct: 186 AKAGIGGMDSELQKLLEDVSPMKFERMNRLVEVKDEEITKLKDE--IRLM--SGQWKHKT 241
Query: 467 KIPDGDLGTQLRTDFDSGKELL 532
K + L Q RTD D K++L
Sbjct: 242 KELESQLEKQRRTDQDLKKKVL 263
>At3g12240.1 68416.m01527 serine carboxypeptidase S10 family protein
contains Pfam profile: PF00450 serine carboxypeptidase;
similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]
Length = 436
Score = 28.3 bits (60), Expect = 4.3
Identities = 20/76 (26%), Positives = 33/76 (43%), Gaps = 4/76 (5%)
Frame = -1
Query: 451 VTIVSHQGQVAIIRDISQL---VVFALHVGYVHVVCGGTD-IFILFTIEDINPNQVNFSV 284
+ ++ H +IIR + + F L GY+ V D +F F + NP + V
Sbjct: 16 LVLIQHADSSSIIRYLPGFEGPLPFELETGYIGVGQKEEDQLFYYFIKSENNPEEDPLLV 75
Query: 283 AVLSGFGCGHFNNLAW 236
+ G GC F+ L +
Sbjct: 76 WLTGGPGCSSFSGLVY 91
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 27.9 bits (59), Expect = 5.7
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +3
Query: 108 FKTQQWVTSKTHTSRPETPGPQPPSP 185
F T Q K++ S P +P P PP P
Sbjct: 83 FHTNQTAVIKSYASPPPSPPPPPPPP 108
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +3
Query: 132 SKTHT-SRPETPGPQPPS 182
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
>At3g49180.1 68416.m05375 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
GTP-binding protein beta chain homolog, Nicotiana
tabacum, PIR:T16970
Length = 438
Score = 27.9 bits (59), Expect = 5.7
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1
Query: 592 SCVCFDGDDALLTAGFQHGA 533
+C+ F GDD+LL +G Q G+
Sbjct: 124 TCLVFSGDDSLLVSGSQDGS 143
>At3g06490.1 68416.m00753 myb family transcription factor (MYB108)
identical to transcription factor MYB108 GI:15375290
from [Arabidopsis thaliana]
Length = 323
Score = 27.9 bits (59), Expect = 5.7
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +3
Query: 237 HARLLKCPHPKPESTATLKFTWLGLISSMVKSMKIS--VPPHTTWTYPT*SAKTTN 398
HA+ LKC + T+K+ W+ + ++S S TT T T SA T++
Sbjct: 122 HAKQLKCDVNSQQFKDTMKYLWMPRLVERIQSASASSAAAATTTTTTTTGSAGTSS 177
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 5.7
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +3
Query: 135 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 260
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 7.6
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 377 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 526
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 7.6
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +2
Query: 407 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 541
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 7.6
Identities = 17/45 (37%), Positives = 20/45 (44%)
Frame = +3
Query: 111 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 245
K Q V S++H S T P PP P P K S C +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,733,947
Number of Sequences: 28952
Number of extensions: 303461
Number of successful extensions: 1012
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 997
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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