SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31278
         (635 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   360   e-100
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   205   5e-54
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   193   2e-50
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   143   2e-35
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...   106   3e-24
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...   104   9e-24
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    93   3e-20
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    54   2e-08
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    44   2e-05
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.14 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.24 
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   4.0  
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch...    25   9.1  
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    25   9.1  
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo...    25   9.1  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  360 bits (885), Expect = e-100
 Identities = 162/179 (90%), Positives = 174/179 (97%)
 Frame = +3

Query: 48  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 227
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 228 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 407
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 408 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYALPHAILRLDL 584
           I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYE YALPHAI+RLDL
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDL 180



 Score = 33.9 bits (74), Expect = 0.020
 Identities = 15/16 (93%), Positives = 15/16 (93%)
 Frame = +1

Query: 586 AGRDLTDYLMKILTER 633
           AGRDLTDYLMKIL ER
Sbjct: 181 AGRDLTDYLMKILMER 196


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  205 bits (500), Expect = 5e-54
 Identities = 93/179 (51%), Positives = 130/179 (72%), Gaps = 1/179 (0%)
 Frame = +3

Query: 54  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 233
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 234 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 410
           +L ++ PIE GII NW DME+IW + + + +L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 411 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYALPHAILRLDLG 587
            +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+ +++P AI + ++G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIG 186


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  193 bits (470), Expect = 2e-50
 Identities = 90/177 (50%), Positives = 127/177 (71%), Gaps = 2/177 (1%)
 Frame = +3

Query: 60  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 233
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 234 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 413
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 414 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYALPHAILRLDL 584
           FE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE   L H + RLD+
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDV 180


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  143 bits (347), Expect = 2e-35
 Identities = 82/205 (40%), Positives = 114/205 (55%), Gaps = 32/205 (15%)
 Frame = +3

Query: 66  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 194
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 195 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 353
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 354 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 509
           LLTE PLNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186

Query: 510 GDGVSHTVPIYEXYALPHAILRLDL 584
           GDGV+H +P+ E Y +  +I  + L
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPL 211


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score =  106 bits (254), Expect = 3e-24
 Identities = 59/190 (31%), Positives = 107/190 (56%), Gaps = 3/190 (1%)
 Frame = +3

Query: 36  FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 215
           F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +     + +  + VG++
Sbjct: 18  FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72

Query: 216 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 392
              + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+ +TE   NP   R
Sbjct: 73  TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131

Query: 393 EKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEXYALPHA 566
             MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+  SH +P+     +   
Sbjct: 132 STMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSE 191

Query: 567 ILRLDLGWSR 596
             R+  G S+
Sbjct: 192 AKRISWGGSQ 201


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score =  104 bits (250), Expect = 9e-24
 Identities = 58/169 (34%), Positives = 90/169 (53%)
 Frame = +3

Query: 51  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 230
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 231 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 410
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 411 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYAL 557
           MFE+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+ + L
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 93.1 bits (221), Expect = 3e-20
 Identities = 49/180 (27%), Positives = 95/180 (52%), Gaps = 2/180 (1%)
 Frame = +3

Query: 51  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 230
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 231 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 404
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 405 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYALPHAILRLDL 584
           +  FE  + PA Y+  QAV   +A+ ++T +++D G   +   P+ +   +   I +  L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 34/116 (29%), Positives = 54/116 (46%), Gaps = 4/116 (3%)
 Frame = +3

Query: 252 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 419
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 420 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEXYALPHAILRLDLG 587
               P   +A + ++ LYA G   G+V+D G   +   PI +   +  A  +L LG
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLG 227


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 43.6 bits (98), Expect = 2e-05
 Identities = 45/192 (23%), Positives = 79/192 (41%), Gaps = 18/192 (9%)
 Frame = +3

Query: 66  LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 245
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 246 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 413
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 414 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEXY 551
           FE F   A      A L  +  G  T               +V+DSG   +H +P+ +  
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177

Query: 552 ALPHAILRLDLG 587
               A+ R+D+G
Sbjct: 178 VQEQAVKRVDVG 189


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.14
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +3

Query: 111 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 254
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +3

Query: 312  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 455
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 354 LLTEAPLNPKANREKMTQIMFETFN 428
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
           family|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 791

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = -2

Query: 307 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 161
           WC+  S + SQ+  +   +G F    PL L WA   +  +    P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 8/16 (50%), Positives = 8/16 (50%)
 Frame = +1

Query: 538 STXVTLCPTPSXVWTW 585
           ST    CP PS  W W
Sbjct: 52  STKTATCPDPSWEWAW 67


>SPBC1683.09c |frp1||ferric-chelate reductase
           Frp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 564

 Score = 25.0 bits (52), Expect = 9.1
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = +3

Query: 171 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 257
           +M+G     +Y+G  AQ KR +LT +  I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,453,759
Number of Sequences: 5004
Number of extensions: 49092
Number of successful extensions: 180
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -