Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31258
         (608 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    67   2e-12
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    55   7e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    44   2e-05
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    38   0.002
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    36   0.006
SPBC21H7.07c |his5||imidazoleglycerol phosphate dehydratase|Schi...    31   0.13 
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    28   0.93 
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo...    25   6.5  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    25   6.5  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 40/114 (35%), Positives = 62/114 (54%), Gaps = 6/114 (5%)
 Frame = +1

Query: 82  GIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDEDD 249
           G I  N+L     SLG+  +  EL +M+ E     +G I+FT+ LT+ A +M   +D ++
Sbjct: 27  GNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-DTDNEE 85

Query: 250 VVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYID 405
            V  AFK FD++G   I  E L H L + G++ S +EV +   + D D  G I+
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVIN 139



 Score = 29.9 bits (64), Expect = 0.30
 Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
 Frame = +1

Query: 277 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTASAE 444
           D  G ID +E L        D  + +EV EA+   D D  GYI   +L  +LT+  E
Sbjct: 60  DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116



 Score = 29.5 bits (63), Expect = 0.40
 Identities = 14/38 (36%), Positives = 22/38 (57%)
 Frame = +3

Query: 21  SQKQVAEFKEAFQLMDHDKVWHHRQERLARHLRLPGQA 134
           + +Q+AEF+EAF L D D+  +     L   +R  GQ+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQS 44


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 55.2 bits (127), Expect = 7e-09
 Identities = 32/95 (33%), Positives = 49/95 (51%), Gaps = 2/95 (2%)
 Frame = +1

Query: 82  GIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVIN 261
           G IG+ D++    SL + ASE  ++ M    + PIN    LT   + +   S  +D ++ 
Sbjct: 63  GNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND-LLE 121

Query: 262 AFKTFD--EEGKIDSERLRHALMTWGDKFSADEVD 360
           AF TFD  + GKI    +R AL + GD+    EV+
Sbjct: 122 AFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156



 Score = 26.6 bits (56), Expect = 2.8
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = +3

Query: 9   FSMFSQKQVAEFKEAFQLMDHD 74
           F+  +  Q+ E KEAF L+D D
Sbjct: 39  FAQLTSSQIQELKEAFALLDKD 60


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 28/120 (23%), Positives = 58/120 (48%), Gaps = 2/120 (1%)
 Frame = +1

Query: 82  GIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVIN 261
           G+I  + + +   SLG   ++ EL ++  E    I+  + ++  +N++     E++  I 
Sbjct: 24  GLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEE-YIK 82

Query: 262 AFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 435
           AF+ FD++  G I++ +    + T G+K S +EV     + D  + G  D    +  + A
Sbjct: 83  AFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQRIMA 142


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 29/123 (23%), Positives = 57/123 (46%), Gaps = 4/123 (3%)
 Frame = +1

Query: 82  GIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGS--DEDDVV 255
           G I K  +     + G+  +  E+ E+       ++  Q L +  NR +G     + +  
Sbjct: 21  GRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NRPNGFDMPGDPEEF 79

Query: 256 INAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 429
           +  F+ FD++  G I    LR+ L + G+K S +E+DE    + + D G ++    + M+
Sbjct: 80  VKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD-GMVNYHDFVQMI 138

Query: 430 TAS 438
            A+
Sbjct: 139 LAN 141


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 35.5 bits (78), Expect = 0.006
 Identities = 27/124 (21%), Positives = 56/124 (45%), Gaps = 6/124 (4%)
 Frame = +1

Query: 88  IGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDEDDVV 255
           I  ++LRA   +LG  A + E+ +++ +      G +     + +   ++    D  + +
Sbjct: 54  IDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVE-RDPLEEI 112

Query: 256 INAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAML 429
             AF+ FD++  GKI    LR       +     E++   ++ D+D  G I+  + IA++
Sbjct: 113 KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172

Query: 430 TASA 441
              A
Sbjct: 173 MDEA 176


>SPBC21H7.07c |his5||imidazoleglycerol phosphate
           dehydratase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 216

 Score = 31.1 bits (67), Expect = 0.13
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 509 GWRLRPYSRDGIITRFHHLPSSSALAVSMA 420
           GW LR YSR  +I   HH    +A+A+ +A
Sbjct: 71  GWSLRLYSRGDLIIDDHHTAEDTAIALGIA 100


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 28.3 bits (60), Expect = 0.93
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +1

Query: 349 DEVDEAYDQMDIDDKGYID 405
           +E +EA+D  D+  KGYID
Sbjct: 12  EEAEEAFDLFDVTHKGYID 30


>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
           Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 457

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 9/26 (34%), Positives = 19/26 (73%)
 Frame = -1

Query: 173 ASPTISSSSFSDASLPRESKVARKSF 96
           +SP++   +++  + P++S V+RKSF
Sbjct: 35  SSPSVPEGTYTVLNNPKQSTVSRKSF 60


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 21/49 (42%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
 Frame = -1

Query: 239 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKS 99
           S P   R+A+ +K S VKL + P+A   + SS+ S ASL  ES   R S
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS 590


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,014,846
Number of Sequences: 5004
Number of extensions: 33950
Number of successful extensions: 161
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 268287866
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)