Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31251
(581 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 68 9e-13
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 65 8e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.093
SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy... 28 1.1
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 27 1.5
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 26 4.6
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 26 4.6
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 25 8.1
SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|... 25 8.1
SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 25 8.1
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 25 8.1
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 68.1 bits (159), Expect = 9e-13
Identities = 41/132 (31%), Positives = 66/132 (50%), Gaps = 1/132 (0%)
Frame = +1
Query: 13 SQHSEKALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKD 192
SQ T+ N L + R + + + EA++WIE LG P FE L++
Sbjct: 12 SQAGAPVGTKGSNTRLAAKQRETLQAYDYLCRVDEAKKWIEECLGTDLGPTSTFEQSLRN 71
Query: 193 GTVLCQLINKLKPGSVPKINTTGG-QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKK 369
G VL L+ K +P + KI + QF+ +NI F I G+P+I F+ D++E K
Sbjct: 72 GVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELTDIYEGK 131
Query: 370 DIAQVVSTLFAL 405
++ +V+ + AL
Sbjct: 132 NLPKVIYCIHAL 143
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 64.9 bits (151), Expect = 8e-12
Identities = 47/146 (32%), Positives = 74/146 (50%), Gaps = 2/146 (1%)
Frame = +1
Query: 109 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 288
EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI
Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64
Query: 289 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 465
+ F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A
Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124
Query: 466 DECKRDFS-DEVLKAGQTVIGLQAGS 540
++ R FS + + + V LQ GS
Sbjct: 125 EKKPRVFSAQQQREFREGVNSLQYGS 150
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 31.5 bits (68), Expect = 0.093
Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
Frame = +1
Query: 115 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 294
EA++W+E ++ + F D L +G VLCQL K P + ++++ E T
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125
Query: 295 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 405
+ +A + G+ F+T DL + +I +V+ L AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 278
Score = 27.9 bits (59), Expect = 1.1
Identities = 20/81 (24%), Positives = 35/81 (43%)
Frame = +1
Query: 25 EKALTQSHNMSLERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVL 204
EK ++ S++ + + ++A K K + E +K P E V+K+ V
Sbjct: 165 EKGKSKPSKKSVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVS 223
Query: 205 CQLINKLKPGSVPKINTTGGQ 267
+ +K KP P N+T Q
Sbjct: 224 DRKKSKKKPKKTPVSNSTASQ 244
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 27.5 bits (58), Expect = 1.5
Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
Frame = -1
Query: 233 PGFSLLMSWQR----TVPSLRTSSNSSPGGNLAPRTPSIHSWASFSFS 102
PG L QR T P L TSS++ PGG + + HS S S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSS 257
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.8 bits (54), Expect = 4.6
Identities = 31/108 (28%), Positives = 47/108 (43%), Gaps = 16/108 (14%)
Frame = +1
Query: 97 NPEKEKEAQEWIEGVL------GAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV--- 240
N E+ +E + I VL G++ P E FE D KDG +L +LIN P ++
Sbjct: 111 NEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDER 170
Query: 241 ----PKINTTGGQFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKD 372
+ N FK +EN ++ KA G I D+ E ++
Sbjct: 171 VLNKQRNNKPLDNFKCIENNNVVINSAKAMGGISITNIGAGDILEGRE 218
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 25.8 bits (54), Expect = 4.6
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +1
Query: 61 ERQVRAKIASKRNPEKEKEAQEWIEGVLGAKFPPGELFEDV 183
ER+ AK A+K EK+K + ++ EG G + PP E+V
Sbjct: 665 EREA-AKAATKSEAEKQKPSGKFEEGT-GGRAPPPPPAEEV 703
>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 8.1
Identities = 10/45 (22%), Positives = 20/45 (44%)
Frame = +3
Query: 240 PQDQHHRWTVQNDGEHHKLPVCNQSIRCTRHRCVPNRRSMGEKRH 374
P+ +++++ +HHKL + RC P +K+H
Sbjct: 206 PRSSTTSYSIRDADKHHKLTTSGVTKMNITERCKPKTTIQTDKKH 250
>SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 376
Score = 25.0 bits (52), Expect = 8.1
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = -3
Query: 477 FAFVSGLGSEAGAAPFRVSVSLTTEGKQCADYLGDVFFL 361
FAFVS EA + F ++ G Q + + +FF+
Sbjct: 51 FAFVSEKSLEAASVKFVATIIAERMGAQFSSFYDKIFFV 89
>SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 429
Score = 25.0 bits (52), Expect = 8.1
Identities = 22/87 (25%), Positives = 40/87 (45%), Gaps = 4/87 (4%)
Frame = +1
Query: 304 AIKAYGVPDIDVFQTVD-LWEKKDIAQVVSTL--FALGRETYRH-AEWSGPCLGPKPADE 471
A+ YG+PDI +F ++D + K+ + +ST F+ ++ A W P E
Sbjct: 304 AMILYGIPDIRLFWSLDERFSKQFLPNKISTFKPFSKYPACFKDIAFWINNDFNPNDFYE 363
Query: 472 CKRDFSDEVLKAGQTVIGLQAGSNKGS 552
RD +++++ + A S K S
Sbjct: 364 IIRDVCQDMVESVNLIDQYTAKSGKTS 390
>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1159
Score = 25.0 bits (52), Expect = 8.1
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -3
Query: 465 SGLGSEAGAAPFRVSVSLTTEGKQ 394
SG G+ AG++PF S +LT E K+
Sbjct: 656 SGFGNTAGSSPF--SFNLTKESKE 677
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.131 0.383
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,604,155
Number of Sequences: 5004
Number of extensions: 57503
Number of successful extensions: 114
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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