BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31227 (454 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 268 2e-73 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 149 3e-37 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 130 1e-31 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 1e-23 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 87 2e-18 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 84 1e-17 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 75 5e-15 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 32 0.036 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 32 0.047 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.083 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.44 SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 5.4 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 5.4 SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.2 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 7.2 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 25 7.2 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 24 9.5 SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 24 9.5 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 24 9.5 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 268 bits (658), Expect = 2e-73 Identities = 117/133 (87%), Positives = 128/133 (96%) Frame = +1 Query: 55 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 234 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 235 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 414 RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 415 MMFETLNSPAMYV 453 ++FET N+PA YV Sbjct: 122 IIFETFNAPAFYV 134 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 149 bits (360), Expect = 3e-37 Identities = 68/130 (52%), Positives = 91/130 (70%), Gaps = 1/130 (0%) Frame = +1 Query: 61 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 240 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 241 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQM 417 +L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127 Query: 418 MFETLNSPAM 447 +ETLN PA+ Sbjct: 128 FYETLNVPAL 137 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 130 bits (314), Expect = 1e-31 Identities = 61/131 (46%), Positives = 89/131 (67%), Gaps = 2/131 (1%) Frame = +1 Query: 67 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 240 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 241 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQMM 420 +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 421 FETLNSPAMYV 453 FE +YV Sbjct: 124 FERYGFGGVYV 134 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 103 bits (247), Expect = 1e-23 Identities = 57/151 (37%), Positives = 80/151 (52%), Gaps = 24/151 (15%) Frame = +1 Query: 73 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 201 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 202 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 360 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 361 LLTEAPLNPKANREKMTQMMFETLNSPAMYV 453 LLTE PLNP NRE ++MFE+ N +Y+ Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYI 157 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 86.6 bits (205), Expect = 2e-18 Identities = 47/132 (35%), Positives = 72/132 (54%) Frame = +1 Query: 58 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 237 D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 238 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQM 417 G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 418 MFETLNSPAMYV 453 MFE+L PA Y+ Sbjct: 121 MFESLRCPATYL 132 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 83.8 bits (198), Expect = 1e-17 Identities = 48/149 (32%), Positives = 86/149 (57%), Gaps = 1/149 (0%) Frame = +1 Query: 4 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 183 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK-- 61 Query: 184 VGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 360 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ Sbjct: 62 --LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118 Query: 361 LLTEAPLNPKANREKMTQMMFETLNSPAM 447 +TE NP R MT+++FE N+P++ Sbjct: 119 CMTEPLANPTYVRSTMTELLFELYNAPSV 147 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 74.9 bits (176), Expect = 5e-15 Identities = 40/134 (29%), Positives = 74/134 (55%), Gaps = 2/134 (1%) Frame = +1 Query: 58 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 237 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 238 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 411 I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 412 QMMFETLNSPAMYV 453 + FE L+ PA Y+ Sbjct: 122 EAAFEQLHVPAFYL 135 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 32.3 bits (70), Expect = 0.036 Identities = 29/122 (23%), Positives = 53/122 (43%), Gaps = 4/122 (3%) Frame = +1 Query: 73 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 252 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 253 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQMM 420 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 421 FE 426 FE Sbjct: 118 FE 119 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 31.9 bits (69), Expect = 0.047 Identities = 19/69 (27%), Positives = 30/69 (43%), Gaps = 4/69 (5%) Frame = +1 Query: 259 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQMMFE 426 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 427 TLNSPAMYV 453 P + Sbjct: 172 ECQVPGFTI 180 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.083 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 118 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 261 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 28.7 bits (61), Expect = 0.44 Identities = 13/43 (30%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Frame = +1 Query: 319 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQMMFETLNSP 441 +Y L E+HP+LLT+ A L P+ + ++ ++ ++ N+P Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441 >SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Schizosaccharomyces pombe|chr 3|||Manual Length = 791 Score = 25.0 bits (52), Expect = 5.4 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -3 Query: 314 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 168 WC+ S + SQ+ + +G F PL L WA + + P ITPWW Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 5.4 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +1 Query: 178 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 264 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 >SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 24.6 bits (51), Expect = 7.2 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%) Frame = +1 Query: 85 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 237 N +GM + R F S VG + VG Q+DSY+ D++ S+R Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 24.6 bits (51), Expect = 7.2 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = -2 Query: 282 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 178 GDD+VLD V +D L L AH D H Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 24.6 bits (51), Expect = 7.2 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +1 Query: 361 LLTEAPLNPKANREKMTQMMFETLN 435 +L APL+ + +KM +++FET N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 24.2 bits (50), Expect = 9.5 Identities = 12/41 (29%), Positives = 26/41 (63%), Gaps = 3/41 (7%) Frame = +1 Query: 274 IITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 387 +I + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 271 VIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 24.2 bits (50), Expect = 9.5 Identities = 8/31 (25%), Positives = 17/31 (54%) Frame = +1 Query: 256 YPIEHGIITNWDDMEKIWHHTFYNELRVAPE 348 YP G++ +W ++E + E+R+ P+ Sbjct: 244 YPRTDGLLFSWSELESMNSENMKPEIRLIPK 274 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 24.2 bits (50), Expect = 9.5 Identities = 10/26 (38%), Positives = 15/26 (57%) Frame = -3 Query: 161 RPTMDGKTARGASSPAKPALHMPEPL 84 RP+ DG+ A G A P++ P P+ Sbjct: 950 RPSNDGRKASGPRPAAPPSIPPPLPV 975 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.409 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,690,861 Number of Sequences: 5004 Number of extensions: 34432 Number of successful extensions: 125 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 168258430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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