Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31224
(444 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol p... 31 0.26
At4g11900.1 68417.m01893 S-locus lectin protein kinase family pr... 30 0.81
At5g55110.1 68418.m06870 stigma-specific Stig1 family protein si... 29 1.1
At5g09670.2 68418.m01119 loricrin-related contains weak similari... 28 2.5
At5g09670.1 68418.m01118 loricrin-related contains weak similari... 28 2.5
At4g24180.1 68417.m03470 pathogenesis-related thaumatin family p... 28 3.3
At5g64550.1 68418.m08112 loricrin-related contains weak similari... 27 4.3
At3g06180.1 68416.m00710 expressed protein 27 4.3
At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:... 27 4.3
At3g17350.1 68416.m02217 expressed protein 27 5.7
At3g02380.1 68416.m00223 zinc finger protein CONSTANS-LIKE 2 (CO... 27 5.7
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 27 5.7
At5g65000.2 68418.m08176 nucleotide-sugar transporter family pro... 27 7.5
At5g65000.1 68418.m08175 nucleotide-sugar transporter family pro... 27 7.5
At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /... 27 7.5
At4g11190.1 68417.m01812 disease resistance-responsive family pr... 27 7.5
At5g43790.1 68418.m05355 pentatricopeptide (PPR) repeat-containi... 26 10.0
At3g22760.1 68416.m02870 CXC domain containing TSO1-like protein... 26 10.0
At3g13810.1 68416.m01744 zinc finger (C2H2 type) family protein ... 26 10.0
At1g31840.1 68414.m03912 pentatricopeptide (PPR) repeat-containi... 26 10.0
>At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol
protease, putative contains similarity to cysteine
proteinase RD21A (thiol protease) GI:435619, SP:P43297
from [Arabidopsis thaliana]
Length = 452
Score = 31.5 bits (68), Expect = 0.26
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = +2
Query: 242 KETRC--RKHGGECFSKGYCSQSLIYEEASDCPEGNDCC 352
K T C ++ G+C+S G C YE A+ C +G+ CC
Sbjct: 372 KSTCCCLYEYNGKCYSWGCCP----YESATCCDDGSSCC 406
>At4g11900.1 68417.m01893 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 849
Score = 29.9 bits (64), Expect = 0.81
Identities = 17/65 (26%), Positives = 28/65 (43%)
Frame = -3
Query: 286 FRETFSAVLPTSGLFYGHSVTAFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFPAARILL 107
F ET + S + FDS L+LR +S + + W H + T P +I L
Sbjct: 138 FHETVWSTGVNSSMSKDVQAVLFDSGNLVLRDGPNSSAAVLWQSFDHPSDTWLPGGKIRL 197
Query: 106 FNGIY 92
+ ++
Sbjct: 198 GSQLF 202
>At5g55110.1 68418.m06870 stigma-specific Stig1 family protein
similar to stigma-specific protein STIG1 [Nicotiana
tabacum] GI:496647; contains Pfam profile PF04885:
Stigma-specific protein, Stig1
Length = 163
Score = 29.5 bits (63), Expect = 1.1
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +2
Query: 185 GLCPKQQKDGIECCYGVSVKETRCRKHGGEC 277
GLC + K G CC G V ++H GEC
Sbjct: 115 GLCKNKCKFGQTCCRGQCVYVAYDKRHCGEC 145
>At5g09670.2 68418.m01119 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 546
Score = 28.3 bits (60), Expect = 2.5
Identities = 21/68 (30%), Positives = 27/68 (39%), Gaps = 6/68 (8%)
Frame = +2
Query: 86 CEIDSIEQEDPCRREGG--LCTVAEDCPSDIRARTGLCPKQ---QKDGIE-CCYGVSVKE 247
C + + D C GG C E C R R+GLC K ++ IE C +
Sbjct: 211 CTKSAEGKTDFCISHGGGRRCEFLEGCDKAARGRSGLCIKHGGGKRCNIEDCTRSAEGQA 270
Query: 248 TRCRKHGG 271
C HGG
Sbjct: 271 GLCISHGG 278
>At5g09670.1 68418.m01118 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 546
Score = 28.3 bits (60), Expect = 2.5
Identities = 21/68 (30%), Positives = 27/68 (39%), Gaps = 6/68 (8%)
Frame = +2
Query: 86 CEIDSIEQEDPCRREGG--LCTVAEDCPSDIRARTGLCPKQ---QKDGIE-CCYGVSVKE 247
C + + D C GG C E C R R+GLC K ++ IE C +
Sbjct: 211 CTKSAEGKTDFCISHGGGRRCEFLEGCDKAARGRSGLCIKHGGGKRCNIEDCTRSAEGQA 270
Query: 248 TRCRKHGG 271
C HGG
Sbjct: 271 GLCISHGG 278
>At4g24180.1 68417.m03470 pathogenesis-related thaumatin family
protein similar to SP|P28493 Pathogenesis-related
protein 5 precursor (PR-5) {Arabidopsis thaliana};
contains Pfam profile PF00314: Thaumatin family
Length = 255
Score = 27.9 bits (59), Expect = 3.3
Identities = 17/72 (23%), Positives = 27/72 (37%), Gaps = 4/72 (5%)
Frame = +2
Query: 110 EDPCRREGGLCTVAEDCPSDIRARTGLCPKQQKDGIE----CCYGVSVKETRCRKHGGEC 277
E C G + + + CP+++R +G K + CC G T C+
Sbjct: 158 EGTCLTTGCVTDLNQKCPTELRFGSGSACKSACEAFGSPEYCCSGAYASPTECKPSMYSE 217
Query: 278 FSKGYCSQSLIY 313
K C +S Y
Sbjct: 218 IFKSACPRSYSY 229
>At5g64550.1 68418.m08112 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 634
Score = 27.5 bits (58), Expect = 4.3
Identities = 23/76 (30%), Positives = 30/76 (39%), Gaps = 8/76 (10%)
Frame = +2
Query: 86 CEIDSIEQEDPCRREGG--LCTVAEDCPSDIRARTGLCPKQ---QKDGIE-CCYGVSVKE 247
C + + D C GG C E C R ++GLC K ++ IE C +
Sbjct: 271 CTKSAEGKTDLCISHGGGRRCGFPEGCAKAARGKSGLCIKHGGGKRCRIESCTRSAEGQA 330
Query: 248 TRCRKHGG--ECFSKG 289
C HGG C S G
Sbjct: 331 GLCISHGGGRRCQSSG 346
>At3g06180.1 68416.m00710 expressed protein
Length = 241
Score = 27.5 bits (58), Expect = 4.3
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -1
Query: 180 LALISDGQSSATVHKPPSLRHGSSCSMESISQKSSS 73
L L+SD + S T PPS S S S S SSS
Sbjct: 4 LLLLSDEEHSTTNSMPPSSSASRSASNHSSSSSSSS 39
>At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:
CHX6a and CHX6b were originally 1 gene but were split
pased on alignments with other family members; may be a
pseudogene and requires futher investigation; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 818
Score = 27.5 bits (58), Expect = 4.3
Identities = 15/56 (26%), Positives = 27/56 (48%)
Frame = -3
Query: 247 LFYGHSVTAFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFPAARILLFNGIYFTEI 80
+ + S++AF S+ LL+ S+ LS + T A + FN IY+ ++
Sbjct: 193 IIFLQSISAFTSIDTLLKDLQIKHSEFGRIALSGAMVTDMLAFGVTFFNAIYYEKL 248
>At3g17350.1 68416.m02217 expressed protein
Length = 278
Score = 27.1 bits (57), Expect = 5.7
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = +2
Query: 185 GLCPKQQKDGIECCYGVS-VKETRCRKHGGEC 277
G+ P GIE Y V+ + RCRK GG C
Sbjct: 213 GVGPLDWSYGIELSYSVTEIGCDRCRKSGGTC 244
>At3g02380.1 68416.m00223 zinc finger protein CONSTANS-LIKE 2 (COL2)
identical to putative flowering-time gene CONSTANS
(COL2) GB:AAB67879 GI:1507699 SP:Q96502 (Arabidopsis
thaliana)
Length = 347
Score = 27.1 bits (57), Expect = 5.7
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = -2
Query: 383 INYPFIVTLKYNNHSLRDSLKLLHISKIDCSSLSRNILRRASDI 252
INY F YNN+SL+D +S +D S + + ASDI
Sbjct: 207 INYGFSSGAHYNNNSLKDLNHSASVSSMDISVVPES---TASDI 247
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 27.1 bits (57), Expect = 5.7
Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +2
Query: 59 FYGNSEDDFCEIDSIEQEDPCRREGGLCTVAEDCPSDIRAR-TGLCPKQQ 205
F N+E +C + Q P +EG L +C ++R + TG+ PK++
Sbjct: 4289 FCENNEKSYCRDVLLSQFSPVFKEGKLLAENLNCLLNVRDQSTGMEPKER 4338
>At5g65000.2 68418.m08176 nucleotide-sugar transporter family
protein low similarity to SP|Q9Y2D2 UDP
N-acetylglucosamine transporter {Homo sapiens}; contains
Pfam profile PF04142: Nucleotide-sugar transporter
Length = 260
Score = 26.6 bits (56), Expect = 7.5
Identities = 16/57 (28%), Positives = 25/57 (43%)
Frame = -3
Query: 265 VLPTSGLFYGHSVTAFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFPAARILLFNGI 95
V+ TS + V ++ L+ R S G WT++ T + PAA L N +
Sbjct: 43 VIVTSSVLTCEIVKVICALILMARNGSLKGLAKEWTLMGSLTASGLPAAIYALQNSL 99
>At5g65000.1 68418.m08175 nucleotide-sugar transporter family
protein low similarity to SP|Q9Y2D2 UDP
N-acetylglucosamine transporter {Homo sapiens}; contains
Pfam profile PF04142: Nucleotide-sugar transporter
Length = 325
Score = 26.6 bits (56), Expect = 7.5
Identities = 16/57 (28%), Positives = 25/57 (43%)
Frame = -3
Query: 265 VLPTSGLFYGHSVTAFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFPAARILLFNGI 95
V+ TS + V ++ L+ R S G WT++ T + PAA L N +
Sbjct: 43 VIVTSSVLTCEIVKVICALILMARNGSLKGLAKEWTLMGSLTASGLPAAIYALQNSL 99
>At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /
cystatin, putative similar to cysteine proteinase
inhibitor [Glycine max] GI:1944342; contains Pfam
profile PF00031: Cystatin domain
Length = 232
Score = 26.6 bits (56), Expect = 7.5
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = -1
Query: 132 PSLRHGSSCSMESISQKSSSLFP 64
P ++ + +M+S+ QKS+SLFP
Sbjct: 162 PEVQEAAKHAMKSLQQKSNSLFP 184
>At4g11190.1 68417.m01812 disease resistance-responsive family
protein / dirigent family protein similar to dirigent
protein [Forsythia x intermedia] gi|6694693|gb|AAF25357;
similar to disease resistance response protein 206-d
[Pisum sativum] gi|508844|gb|AAB18669
Length = 184
Score = 26.6 bits (56), Expect = 7.5
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -3
Query: 322 SFFIYQRLTAVAFRETFSAVLPTSG-LFYGHSVTAFD 215
S +YQ T ++FR+ F+ P +FY H+V A+D
Sbjct: 14 SVLLYQSTTVLSFRQPFNLAKPCKRFVFYLHNV-AYD 49
>At5g43790.1 68418.m05355 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 460
Score = 26.2 bits (55), Expect = 10.0
Identities = 14/35 (40%), Positives = 23/35 (65%)
Frame = -2
Query: 368 IVTLKYNNHSLRDSLKLLHISKIDCSSLSRNILRR 264
I+T+ ++H+ S KLLH+S C S + +ILR+
Sbjct: 32 IITIGLSHHTYPLS-KLLHLSSTVCLSYALSILRQ 65
>At3g22760.1 68416.m02870 CXC domain containing TSO1-like protein 1
(SOL1) identical to CXC domain containing TSO1-like
protein 1 (SOL1) [Arabidopsis thaliana] GI:7767427;
contains Pfam profile PF03638: Tesmin/TSO1-like CXC
domain; supporting cDNA
gi|7767426|gb|AF205142.1|AF205142
Length = 609
Score = 26.2 bits (55), Expect = 10.0
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +2
Query: 197 KQQKDGIECCYGVSVKETRCRKHGGECFSKG-YC 295
+Q +G C + K+++C K ECF+ G YC
Sbjct: 318 EQSGEGDSSCKRCNCKKSKCLKLYCECFAAGFYC 351
>At3g13810.1 68416.m01744 zinc finger (C2H2 type) family protein
similar to finger protein pcp1 GB:S48856 from [Solanum
tuberosum] contains Pfam domain, PF00096: Zinc finger,
C2H2 type
Length = 513
Score = 26.2 bits (55), Expect = 10.0
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +2
Query: 239 VKETRCRKHGGECFSKGYCSQSLIYEEASDCPEGNDCC 352
+K+ CRKHG + + CS+ Y SDC + C
Sbjct: 163 IKKHFCRKHGEKKWKCDKCSKK--YAVQSDCKAHSKTC 198
>At1g31840.1 68414.m03912 pentatricopeptide (PPR) repeat-containing
protein contains multiple PPR domains: Pfam profile:
PF01535: PPR repeat
Length = 690
Score = 26.2 bits (55), Expect = 10.0
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 3/53 (5%)
Frame = -3
Query: 241 YGHSVTAFDSVFLLLRTESSSGSDI---RWTVLSHSTQTTFPAARILLFNGIY 92
YG ++ ++ +LL ES S + RW +S + + A +L+ NG++
Sbjct: 63 YGSNLQRNETNLVLLSLESEPNSALKYFRWAEISGKDPSFYTIAHVLIRNGMF 115
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,036,567
Number of Sequences: 28952
Number of extensions: 195321
Number of successful extensions: 676
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 676
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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