Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31219
(537 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 101 7e-23
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 59 4e-10
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 58 1e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 4e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 40 3e-04
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 34 0.015
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 32 0.062
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 31 0.083
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 31 0.11
SPAC25B8.11 |||transcription factor|Schizosaccharomyces pombe|ch... 26 4.1
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 4.1
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 5.4
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 25 5.4
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 5.4
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 7.2
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 25 7.2
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 7.2
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 25 9.5
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 25 9.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 101 bits (242), Expect = 7e-23
Identities = 51/148 (34%), Positives = 86/148 (58%)
Frame = +3
Query: 54 LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 233
L EQIA R+AF FD ++ G+I + + ++R +GQ L+++I EVDAD +G +
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 234 EFPEFVTLAAKFIVEEDAEAMHKELREAFRLYDKESNGYIPTSSLREIXXXXXXXXXXXX 413
+F EF+T+ A+ + + D E +E+REAF+++DK+ NGYI L +
Sbjct: 66 DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 414 XXXXIQEIDTDGSGTADFDEFLEMMTGE 497
I+E DTDG G +++EF +++ +
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVISSK 150
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 59.3 bits (137), Expect = 4e-10
Identities = 38/149 (25%), Positives = 67/149 (44%)
Frame = +3
Query: 51 ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 230
E+ EQ + +AF FD ++ +I + +R +G K + +++ + D G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 231 LEFPEFVTLAAKFIVEEDAEAMHKELREAFRLYDKESNGYIPTSSLREIXXXXXXXXXXX 410
L+ +FV + + IVE D +E++ AF L+D + G I +LR +
Sbjct: 90 LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146
Query: 411 XXXXXIQEIDTDGSGTADFDEFLEMMTGE 497
I+E D D G + EF+ +M E
Sbjct: 147 ELEAMIEEFDLDQDGEINEQEFIAIMMDE 175
Score = 39.1 bits (87), Expect = 4e-04
Identities = 21/75 (28%), Positives = 40/75 (53%)
Frame = +3
Query: 33 TEDWADELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVD 212
TE + P E+I ++AF+ FD + +G I + + + + + + + LE +IEE D
Sbjct: 100 TEKIVERDPLEEI---KRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFD 156
Query: 213 ADKSGRLEFPEFVTL 257
D+ G + EF+ +
Sbjct: 157 LDQDGEINEQEFIAI 171
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 57.6 bits (133), Expect = 1e-09
Identities = 42/149 (28%), Positives = 67/149 (44%), Gaps = 5/149 (3%)
Frame = +3
Query: 63 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 242
E+I +RK F D N+SGSI + I + P + L VD D G ++F
Sbjct: 21 EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76
Query: 243 EFVTLAAKFIVEEDAEAMHKELREAFRLYDKESNGYIPTSSL-----REIXXXXXXXXXX 407
EF+ + F V + E ++L+ AF++YD + +GYI L +
Sbjct: 77 EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQ 133
Query: 408 XXXXXXIQEIDTDGSGTADFDEFLEMMTG 494
I E+D D G F+EF ++++G
Sbjct: 134 QIVDKTIMEVDKDRDGKISFEEFKDIVSG 162
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.6 bits (128), Expect = 4e-09
Identities = 35/140 (25%), Positives = 69/140 (49%)
Frame = +3
Query: 63 EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 242
EQ +++AF +D ++ G IP V +LR +G L +L E+ ++
Sbjct: 6 EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61
Query: 243 EFVTLAAKFIVEEDAEAMHKELREAFRLYDKESNGYIPTSSLREIXXXXXXXXXXXXXXX 422
+F++ + + E ++E +E +AFR++DK+++GYI T+ +
Sbjct: 62 KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 423 XIQEIDTDGSGTADFDEFLE 482
+QE D SG+ D+ +F++
Sbjct: 119 MVQEADPTNSGSFDYYDFVQ 138
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 39.5 bits (88), Expect = 3e-04
Identities = 28/102 (27%), Positives = 52/102 (50%), Gaps = 3/102 (2%)
Frame = +3
Query: 81 RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 260
++AF FD + +G IP + D+LR GQ N + E I E+++ ++ +F+ +
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64
Query: 261 AK---FIVEEDAEAMHKELREAFRLYDKESNGYIPTSSLREI 377
+ F + D E E + F+++DK++ G I LR +
Sbjct: 65 NRPNGFDMPGDPE----EFVKGFQVFDKDATGMIGVGELRYV 102
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 33.9 bits (74), Expect = 0.015
Identities = 21/63 (33%), Positives = 34/63 (53%)
Frame = +3
Query: 189 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMHKELREAFRLYDKESNGYIPTSSL 368
E + DADK+G ++F EF+ + V E ++ +L AF+LYD ++NG I +
Sbjct: 66 EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121
Query: 369 REI 377
I
Sbjct: 122 LRI 124
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 31.9 bits (69), Expect = 0.062
Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = -2
Query: 479 QELVEVGGAASVSV-DLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEF 303
QEL V A S DL D AI ++Q+L F +DL + VT+ + +P +
Sbjct: 1245 QELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSY 1304
Query: 302 FVHGLS 285
+ + +S
Sbjct: 1305 YTYRVS 1310
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 31.5 bits (68), Expect = 0.083
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = +1
Query: 70 SPSSAKPSTGSTTTARVVSPVTSWLIS*E*WGSHS 174
S SSA PS+GS+T +R+ S +TS +I E +GS S
Sbjct: 734 SNSSAYPSSGSSTFSRLSSTLTSSIIPTETFGSTS 768
Score = 27.1 bits (57), Expect = 1.8
Identities = 34/142 (23%), Positives = 58/142 (40%)
Frame = +1
Query: 52 SCPQNRSPSSAKPSTGSTTTARVVSPVTSWLIS*E*WGSHSTRKSSRNLLRRSTPTXXXX 231
S + + SS+ S+ T+++ S +TS ++ S S SS N +TPT
Sbjct: 118 SLASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSATPT-SSA 176
Query: 232 XXXXXXXXXXXXXXXKRTLRPCTKNSGKRSDCTTRKATVTFPRRACARSSGNWTSS*LTM 411
+L + NS T AT T + +S + TSS L
Sbjct: 177 TSSSLSSTAASNSATSSSLASSSLNS-------TTSATATSSSLSSTAASNSATSSSLAS 229
Query: 412 NSMASSKRSTLTEAAPPTSTSS 477
+S+ S+ +T T ++ ++ SS
Sbjct: 230 SSLNSTTSATATSSSISSTVSS 251
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 31.1 bits (67), Expect = 0.11
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3
Query: 90 FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 248
++ + +S + V + +M+ + + LEE ++ DAD SG+L F EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPAC25B8.11 |||transcription factor|Schizosaccharomyces pombe|chr
1|||Manual
Length = 654
Score = 25.8 bits (54), Expect = 4.1
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = -2
Query: 206 LLNKFLED-FLVEWLPHHSQD---ISHEVTGDTTRAVVVEPVE 90
LL L+D F V L HS++ IS+++ G TR + PVE
Sbjct: 433 LLQDILQDLFTVRALTRHSKNNRSISYQIIGFFTRLNSIRPVE 475
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 25.8 bits (54), Expect = 4.1
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +3
Query: 294 MHKELREAFRLYDKESNGYIPTSSLR 371
M +E EAF L+D GYI LR
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.4 bits (53), Expect = 5.4
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +3
Query: 186 LEELIEEVDADKSGRLEFPEFV 251
+ E I EV+ D SGR+E +FV
Sbjct: 56 VREAIREVNVDSSGRVEPEDFV 77
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1811
Score = 25.4 bits (53), Expect = 5.4
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +3
Query: 198 IEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMHKELREA 317
I SG+L FV A K + + A HK+LREA
Sbjct: 118 ISNASNQNSGKLNPQLFVIEAFKHMSKAKATKRHKKLREA 157
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 25.4 bits (53), Expect = 5.4
Identities = 15/36 (41%), Positives = 21/36 (58%)
Frame = +1
Query: 370 ARSSGNWTSS*LTMNSMASSKRSTLTEAAPPTSTSS 477
A ++G+ TSS T + SS S T +A +STSS
Sbjct: 968 ASATGSSTSSIATASVSGSSTSSVATASATDSSTSS 1003
Score = 25.0 bits (52), Expect = 7.2
Identities = 16/38 (42%), Positives = 21/38 (55%)
Frame = +1
Query: 364 ACARSSGNWTSS*LTMNSMASSKRSTLTEAAPPTSTSS 477
A A +G+ TSS T + SS S T +A +STSS
Sbjct: 1005 AAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSS 1042
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.0 bits (52), Expect = 7.2
Identities = 14/60 (23%), Positives = 31/60 (51%)
Frame = +1
Query: 298 TKNSGKRSDCTTRKATVTFPRRACARSSGNWTSS*LTMNSMASSKRSTLTEAAPPTSTSS 477
T ++ S ++ T T+P + ++ + +SS + +S SS + + + PP++ SS
Sbjct: 592 TTSTVSESGSSSASITSTYPSSTLSMTTSHLSSSSVHSSSAHSSSSRSSSMSLPPSAGSS 651
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 25.0 bits (52), Expect = 7.2
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = -2
Query: 194 FLEDFLVEWLPHHSQDISHEVTGDTTRAVVVEPVEGFAEDGDLFWGQL 51
FL + L H D H + + +PVEG + DG L +G++
Sbjct: 1075 FLGPTYYQRLKHLVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEM 1122
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.0 bits (52), Expect = 7.2
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +1
Query: 328 TTRKATVTFPRRACARSSGNWTSS*LTMNSMASSKRSTLTEAAPPTSTSS 477
T+ +T T S+ TS+ + +SS + +T PPTSTSS
Sbjct: 153 TSTSSTDTNSNPLPTTSTSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSS 202
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = +1
Query: 31 TPKTGPMSCPQNRSPSSAKPSTGSTTTARV 120
+P GP+ P +R P A PS + RV
Sbjct: 73 SPHHGPVRSPSSRKPLPASPSRTRDHSLRV 102
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 300 KELREAFRLYDKESNGYI 353
+EL+EAF L DK+ +G I
Sbjct: 48 QELKEAFALLDKDGDGNI 65
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,958,190
Number of Sequences: 5004
Number of extensions: 35541
Number of successful extensions: 201
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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