SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31208
         (509 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g31320.1 68415.m03824 poly [ADP-ribose] polymerase, putative ...   105   1e-23
At4g02390.1 68417.m00323 poly (ADP-ribose) polymerase / NAD(+) A...    68   4e-12
At1g63870.1 68414.m07233 disease resistance protein (TIR-NBS-LRR...    31   0.45 
At5g67540.2 68418.m08516 glycosyl hydrolase family protein 43 co...    30   1.0  
At5g67540.1 68418.m08517 glycosyl hydrolase family protein 43 co...    30   1.0  
At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA) si...    30   1.0  
At4g19610.1 68417.m02881 RNA recognition motif (RRM)-containing ...    29   1.4  
At5g23650.1 68418.m02773 myb family transcription factor contain...    29   1.8  
At3g60200.1 68416.m06726 expressed protein hypothetical proteins...    29   2.4  
At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR...    29   2.4  
At5g18410.2 68418.m02167 expressed protein similar to p53 induci...    28   3.2  
At5g18410.1 68418.m02166 expressed protein similar to p53 induci...    28   3.2  
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains...    28   3.2  
At3g51540.1 68416.m05644 expressed protein mucin 5AC, Homo sapie...    27   5.5  
At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (...    27   5.5  
At3g25230.1 68416.m03152 peptidyl-prolyl cis-trans isomerase / F...    27   5.5  
At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containi...    27   5.5  
At1g56540.1 68414.m06502 disease resistance protein (TIR-NBS-LRR...    27   7.3  
At1g08440.1 68414.m00933 hypothetical protein contains Pfam prof...    27   7.3  
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin...    27   9.7  
At4g20850.1 68417.m03025 subtilase family protein contains simil...    27   9.7  
At3g47040.1 68416.m05108 glycosyl hydrolase family 3 protein bet...    27   9.7  
At1g52360.1 68414.m05909 coatomer protein complex, subunit beta ...    27   9.7  

>At2g31320.1 68415.m03824 poly [ADP-ribose] polymerase, putative /
           NAD(+) ADP-ribosyltransferase, putative /
           poly[ADP-ribose] synthetase, putative similar to
           poly(ADP)-ribose polymerase [Zea mays] GI:3928871 ;
           contains Pfam profiles PF00644: Poly(ADP-ribose)
           polymerase catalytic domain, PF00645: Poly(ADP-ribose)
           polymerase and DNA-Ligase Zn-finger region, PF02877:
           Poly(ADP-ribose) polymerase, regulatory domain, PF00533:
           BRCA1 C Terminus (BRCT) domain
          Length = 983

 Score =  105 bits (253), Expect = 1e-23
 Identities = 56/146 (38%), Positives = 88/146 (60%), Gaps = 4/146 (2%)
 Frame = +1

Query: 82  KSIPKSKSMYVKSEKSAVKLKIRDGNAVDPASELDEECHVYTDRYKTTYSVVLSLADVVA 261
           + +P  K     + +S V +K++  +AV  AS L E CH+  D   + Y+  LS++D+  
Sbjct: 475 RKLPFDKYKIEDTSESLVTVKVKGRSAVHEASGLQEHCHILEDG-NSIYNTTLSMSDLST 533

Query: 262 QKNSFYKLQILKHNKENRYWLFRSWGRIGT-TIGGSKVEKMKDLDNALDRFQALYEEKTE 438
             NS+Y LQI++ +K +  ++FR WGR+G   IGG+KVE+M   D A+  F+ L+ EKT 
Sbjct: 534 GINSYYILQIIQEDKGSDCYVFRKWGRVGNEKIGGNKVEEMSKSD-AVHEFKRLFLEKTG 592

Query: 439 N---RWKDRNNFVKIAGRYFPLEIDY 507
           N    W+ + NF K  G++ PL+IDY
Sbjct: 593 NTWESWEQKTNFQKQPGKFLPLDIDY 618


>At4g02390.1 68417.m00323 poly (ADP-ribose) polymerase / NAD(+)
           ADP-ribosyltransferase / poly[ADP-ribose] synthetase
           (APP) identical to  SP|Q11207 Poly [ADP-ribose]
           polymerase (EC 2.4.2.30) (PARP) (ADPRT) (NAD(+) ADP-
           ribosyltransferase) (Poly[ADP-ribose] synthetase)
           {Arabidopsis thaliana}
          Length = 637

 Score = 67.7 bits (158), Expect = 4e-12
 Identities = 36/111 (32%), Positives = 58/111 (52%), Gaps = 1/111 (0%)
 Frame = +1

Query: 178 ELDEECHVYTDRYKTTYSVVLSLADVVAQKNSFYKLQILKHNKENRYWLFRSWGRIGTTI 357
           E+  + HV   R    Y  +L+  +V    N F+ LQ+L+ + +  Y ++  WGR+G   
Sbjct: 154 EIKSQYHVL-QRGDDVYDAILNQTNVRDNNNKFFVLQVLESDSKKTYMVYTRWGRVGVK- 211

Query: 358 GGSKVEKMKD-LDNALDRFQALYEEKTENRWKDRNNFVKIAGRYFPLEIDY 507
           G SK++   D  D A++ F   + +KT+N W DR  F+     Y  LE+DY
Sbjct: 212 GQSKLDGPYDSWDRAIEIFTNKFNDKTKNYWSDRKEFIPHPKSYTWLEMDY 262


>At1g63870.1 68414.m07233 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1031

 Score = 31.1 bits (67), Expect = 0.45
 Identities = 18/66 (27%), Positives = 28/66 (42%)
 Frame = -3

Query: 414 LESVQSVIEIFHLLDFTAADRCADPTPRTEQPVSVLFIMFQDLQFVERVLLSDNISEREH 235
           +E++   I+  HLL       C       E P S+ F+M  D + +E V    N  + E 
Sbjct: 771 IETIPECIKSLHLLYILNLSGCRRLASLPELPSSLRFLMADDCESLETVFCPLNTPKAEL 830

Query: 234 DRVRCF 217
           +   CF
Sbjct: 831 NFTNCF 836


>At5g67540.2 68418.m08516 glycosyl hydrolase family protein 43
           contains similarity to xylanase GI:2645416 from
           [Caldicellulosiruptor saccharolyticus]
          Length = 471

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
 Frame = +1

Query: 331 SWGRIGTTIGGSKVEKMKDL--DNALDRFQALYEEKTE 438
           +W   G  +G  +  K  DL   N L+R + +Y EKTE
Sbjct: 211 TWKNEGIVLGAEETNKTHDLHKSNVLERPKVIYNEKTE 248


>At5g67540.1 68418.m08517 glycosyl hydrolase family protein 43
           contains similarity to xylanase GI:2645416 from
           [Caldicellulosiruptor saccharolyticus]
          Length = 466

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
 Frame = +1

Query: 331 SWGRIGTTIGGSKVEKMKDL--DNALDRFQALYEEKTE 438
           +W   G  +G  +  K  DL   N L+R + +Y EKTE
Sbjct: 206 TWKNEGIVLGAEETNKTHDLHKSNVLERPKVIYNEKTE 243


>At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA)
           similar to ribosomal protein L13A GB:O49885 [Lupinus
           luteus]
          Length = 206

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 16/53 (30%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
 Frame = +1

Query: 283 LQILKHNKENRYWLFRSWGRIGTTIGGSKVEKMKDLDN-ALDRFQALYEEKTE 438
           L++L+    ++Y L    GR+ + +G +  + +K+L+N   +R QA+YE K +
Sbjct: 129 LKVLRLQAGHKYCLL---GRLSSEVGWNHYDTIKELENKRKERAQAVYERKKQ 178


>At4g19610.1 68417.m02881 RNA recognition motif (RRM)-containing
           protein contains InterPro entry IPR000504: RNA-binding
           region RNP-1 (RNA recognition motif) (RRM)
          Length = 783

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 26/133 (19%), Positives = 54/133 (40%), Gaps = 1/133 (0%)
 Frame = +1

Query: 61  PQDIIDGKSIPKSKSMYVKSEKSAVK-LKIRDGNAVDPASELDEECHVYTDRYKTTYSVV 237
           P +I++ K++P +       E++ V+ + +     +DP        +V    +KTT   +
Sbjct: 526 PGNILEPKNLPDTNEERSDIEENGVRRVNLEQQVEIDPDVTESNVLNVKNLSFKTTDEGL 585

Query: 238 LSLADVVAQKNSFYKLQILKHNKENRYWLFRSWGRIGTTIGGSKVEKMKDLDNALDRFQA 417
                 + ++     + I+KH K  +Y L   +G +      +     +DL   +    A
Sbjct: 586 KKHFTKLVKQGKILSVTIIKHKKNEKY-LSSGYGFVEFDSVETATSVYRDLQGTVLDGHA 644

Query: 418 LYEEKTENRWKDR 456
           L     EN+  D+
Sbjct: 645 LILRFCENKRSDK 657


>At5g23650.1 68418.m02773 myb family transcription factor contains
           Pfam profile: PF00249 myb-like DNA-binding domain
          Length = 337

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = +1

Query: 382 KDLDNALDRFQALYEEKTENRWK 450
           KD D A ++  A+Y +KTE RWK
Sbjct: 15  KDDDIAFEKALAIYNDKTEIRWK 37


>At3g60200.1 68416.m06726 expressed protein hypothetical proteins
           At2g44600 - Arabidopsis thaliana, EMBL:AAC27462
          Length = 305

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 15/66 (22%), Positives = 30/66 (45%)
 Frame = +2

Query: 176 RNWTRSATCTRTATKQRTRSCSRSLMLSLKRTLSTNCRS*NIIKRTDTGCSVRGVGSAQR 355
           R+W  +   T    KQ T +C    +++ +R     CR  + ++ T+   S   +   +R
Sbjct: 158 RSWISTILSTGRRKKQPTTACYIEDVIAARRPQRIYCRGMSPVRDTEPEQSAESIEELRR 217

Query: 356 SAAVKS 373
           + A K+
Sbjct: 218 TPATKT 223


>At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR
            class), putative domain signature TIR-NBS-LRR exists,
            suggestive of a disease resistance protein.
          Length = 897

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 20/78 (25%), Positives = 33/78 (42%)
 Frame = -3

Query: 414  LESVQSVIEIFHLLDFTAADRCADPTPRTEQPVSVLFIMFQDLQFVERVLLSDNISEREH 235
            +E V   I+  H L +     C       E P S+  +  +D   +ERV  S NI   + 
Sbjct: 773  IEWVTDSIKDLHNLYYLKLSGCKRLVSLPELPCSLECLFAEDCTSLERVSDSLNIPNAQF 832

Query: 234  DRVRCFVAVRVHVALLVQ 181
            + ++CF   R     ++Q
Sbjct: 833  NFIKCFTLDREARRAIIQ 850


>At5g18410.2 68418.m02167 expressed protein similar to p53 inducible
           protein [Homo sapiens] GI:5616320
          Length = 1017

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 11/30 (36%), Positives = 20/30 (66%)
 Frame = -1

Query: 461 LFLSFQRFSVFSSYSAWNLSKALSRSFIFS 372
           +F+S    S+F+ Y +W+ S+ L  SF+F+
Sbjct: 698 IFVSRLSESIFTYYKSWSASELLDPSFLFA 727


>At5g18410.1 68418.m02166 expressed protein similar to p53 inducible
           protein [Homo sapiens] GI:5616320
          Length = 1234

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 11/30 (36%), Positives = 20/30 (66%)
 Frame = -1

Query: 461 LFLSFQRFSVFSSYSAWNLSKALSRSFIFS 372
           +F+S    S+F+ Y +W+ S+ L  SF+F+
Sbjct: 698 IFVSRLSESIFTYYKSWSASELLDPSFLFA 727


>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
           ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
          Length = 1340

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
 Frame = +1

Query: 52  SKLPQDI-IDGKSIPKSKSMYVKSEKSAVKLKIRDGNAVDPASELDEE 192
           S +P+ + I   ++PK +S    S K  VK     G+  DP+++ +E+
Sbjct: 775 SDVPEHVAIPETTVPKEESTGTSSNKKIVKKVAETGDTSDPSAKANEQ 822


>At3g51540.1 68416.m05644 expressed protein mucin 5AC, Homo sapiens,
           PIR:S53363
          Length = 438

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 18/49 (36%), Positives = 24/49 (48%)
 Frame = +2

Query: 230 RSCSRSLMLSLKRTLSTNCRS*NIIKRTDTGCSVRGVGSAQRSAAVKSR 376
           R+ SRSL  S ++T S       I   T T  S +  G AQRS ++  R
Sbjct: 153 RTVSRSLTPSSRKTPSPTSTPSRISTTTSTTPSFKTAGDAQRSRSLTPR 201


>At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (Atm)
            identical to ataxia-telangiectasia mutated protein (Atm)
            [Arabidopsis thaliana] GI:7529272; contains Pfam profile
            PF00855: PWWP domain; contains GA donor splice site at
            exon 73
          Length = 3255

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = +1

Query: 355  IGGSKVEKMKDLDNALDRFQALYEEKTENRWKDRNNFV 468
            +G S    +  L+N L  F  +YEE TE+ W   ++ V
Sbjct: 1595 LGASYDFVIHSLENLLRSFAKVYEEYTEHAWNTHSDTV 1632


>At3g25230.1 68416.m03152 peptidyl-prolyl cis-trans isomerase /
           FK506-binding protein (ROF1) identical to rotamase FKBP
           (ROF1) GB:U49453 [Arabidopsis thaliana] (Mol. Gen.
           Genet. 252 (5), 510-517 (1996))
          Length = 551

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 16/44 (36%), Positives = 20/44 (45%)
 Frame = +1

Query: 361 GSKVEKMKDLDNALDRFQALYEEKTENRWKDRNNFVKIAGRYFP 492
           G K E  KDLD  L +F+A  E+ T     D   F    G + P
Sbjct: 164 GEKWENPKDLDEVLVKFEAKLEDGTVVGKSDGVEFTVKDGHFCP 207


>At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat; gene
           structure supported by cDNA sequence and Brassica genome
           sequence alignments.
          Length = 626

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 6/59 (10%)
 Frame = +1

Query: 346 GTTIGGSKV-EKMKDLDNA----LDRFQA-LYEEKTENRWKDRNNFVKIAGRYFPLEID 504
           G T  G K+ E M+D D      +D +   +Y    ENRW+D   F+    + FP  +D
Sbjct: 402 GRTDDGLKILEMMQDSDTVHGARIDPYNCVIYGFYKENRWEDALEFLLKMEKLFPRAVD 460


>At1g56540.1 68414.m06502 disease resistance protein (TIR-NBS-LRR
            class), putative domain signature TIR-NBS-LRR exists,
            suggestive of a disease resistance protein.
          Length = 1096

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 21/88 (23%), Positives = 35/88 (39%), Gaps = 2/88 (2%)
 Frame = -3

Query: 474  NLDKVIPVFPTVLSFFFVQR--LESVQSVIEIFHLLDFTAADRCADPTPRTEQPVSVLFI 301
            NL     + PT ++   +    +ES+   I+  H L   A   C   T   + P S+ ++
Sbjct: 752  NLKTFSTLLPTSVTELHIDNSGIESITDCIKGLHNLRVLALSNCKKLTSLPKLPSSLKWL 811

Query: 300  MFQDLQFVERVLLSDNISEREHDRVRCF 217
                 + +ERV    N    + D   CF
Sbjct: 812  RASHCESLERVSEPLNTPNADLDFSNCF 839


>At1g08440.1 68414.m00933 hypothetical protein contains Pfam profile
           PF01027: Uncharacterized protein family UPF0005
          Length = 501

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 14/46 (30%), Positives = 25/46 (54%)
 Frame = +1

Query: 133 VKLKIRDGNAVDPASELDEECHVYTDRYKTTYSVVLSLADVVAQKN 270
           V +KI D +++   +E D + HV+T+R    ++    L D   +KN
Sbjct: 457 VTIKINDDDSIHDKNE-DGDIHVHTNRVSCDHTNASDLLDSGVKKN 501


>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
           homolog 1, Arabidopsis thaliana, EMBL:AT08315
           [SP|P29402]
          Length = 530

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 14/50 (28%), Positives = 22/50 (44%)
 Frame = +1

Query: 43  DPASKLPQDIIDGKSIPKSKSMYVKSEKSAVKLKIRDGNAVDPASELDEE 192
           DP  K P+D  +   IP   ++  +       ++I D  A  P   LD+E
Sbjct: 225 DPEDKKPEDWDERAKIPDPNAVKPEDWDEDAPMEIEDEEAEKPEGWLDDE 274


>At4g20850.1 68417.m03025 subtilase family protein contains
           similarity to Tripeptidyl-peptidase II (EC 3.4.14.10)
           (TPP-II) (Tripeptidyl aminopeptidase)
           (Swiss-Prot:P29144) [Homo sapiens]
          Length = 1380

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
 Frame = +2

Query: 224 RTRSCSRSLMLSLKRT--LSTNCRS*NIIKRTDTGCSVRGVGSA 349
           R RSCSRS  + L+R+   +  C S + +  +  GC   G G A
Sbjct: 49  RRRSCSRSRGIRLRRSGFSAMPCSSSDTLTASRVGCGGGGGGGA 92


>At3g47040.1 68416.m05108 glycosyl hydrolase family 3 protein
           beta-D-glucan exohydrolase, Nicotiana tabacum,
           TREMBL:AB017502_1
          Length = 636

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = -1

Query: 437 SVFSSYSAWNLSKALSRSFIFSTLL 363
           +V +SYS+WN SK  S  F+ + LL
Sbjct: 278 TVMASYSSWNGSKLHSDYFLLTELL 302


>At1g52360.1 68414.m05909 coatomer protein complex, subunit beta 2
           (beta prime), putative contains 7 WD-40 repeats
           (PF00400) (1 weak); similar to (SP:O55029) Coatomer
           beta' subunit (Beta'-coat protein) (Beta'-COP) (p102)
           (SP:O55029) [Mus musculus]; similar to GI:298096 from
           [Homo sapiens]
          Length = 926

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 13/43 (30%), Positives = 24/43 (55%)
 Frame = -1

Query: 170 GSTALPSRILSLTADFSDLTYMLLLLGIDFPSIISWGSLEAGS 42
           G  A  SR+  +  +F+ + Y LLL  I++ +++  G L+  S
Sbjct: 566 GYLASQSRVFLVDKEFNVIGYTLLLSLIEYKTLVMRGDLDKAS 608


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.316    0.134    0.394 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,201,008
Number of Sequences: 28952
Number of extensions: 186667
Number of successful extensions: 699
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 697
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)

- SilkBase 1999-2023 -