BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31206 (692 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 31 0.16 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 31 0.16 SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyc... 29 0.48 SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 29 0.84 SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 28 1.5 SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces ... 27 1.9 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 27 2.6 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 27 2.6 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 3.4 SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 27 3.4 SPAC22A12.14c |||BSD domain protein, unknown biological role|Sch... 26 4.5 SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr ... 26 4.5 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 26 5.9 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 5.9 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 5.9 SPBC365.07c |||TATA element modulatory factor homolog |Schizosac... 26 5.9 SPCC188.09c |||glycoprotein |Schizosaccharomyces pombe|chr 3|||M... 26 5.9 SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe... 25 7.9 SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 25 7.9 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 7.9 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 31.1 bits (67), Expect = 0.16 Identities = 18/56 (32%), Positives = 36/56 (64%), Gaps = 2/56 (3%) Frame = +2 Query: 260 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKR--LIEKQAKRK 421 KQ+ E D E + R+ + R + ++++ E + R++ KEE+E +R L+EK+++ K Sbjct: 677 KQQQEADREKMAREQRLREEEEKRILEE-RKRREKLDKEEEERRRRELLEKESEEK 731 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/45 (33%), Positives = 30/45 (66%), Gaps = 2/45 (4%) Frame = +2 Query: 293 KRQDQKRSDLDEQLKEYINEW--RKQRAKEEDELKRLIEKQAKRK 421 ++Q ++ L +Q +E E R+QR +EE+E KR++E++ +R+ Sbjct: 666 QKQKEREKKLKKQQQEADREKMAREQRLREEEE-KRILEERKRRE 709 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 31.1 bits (67), Expect = 0.16 Identities = 23/100 (23%), Positives = 42/100 (42%) Frame = +2 Query: 299 QDQKRSDLDEQLKEYINEWRKQRAKEEDELKRLIEKQAKRKVSXXXXXXXXXXXXXXXXX 478 ++Q + + +E+ K E K+ A E++ KR E+ AKR+ Sbjct: 566 EEQAKREAEEKAKREAEEKAKREA--EEKAKREAEENAKREAEEKAKREAEEKAKREAEE 623 Query: 479 XXXXXXXXXXXXDIEEKRQRLEEAEKKRQAMLQAMKDASK 598 + EEK +R E + KR+A +A ++A + Sbjct: 624 KAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEE 663 Score = 29.1 bits (62), Expect = 0.64 Identities = 22/100 (22%), Positives = 42/100 (42%) Frame = +2 Query: 299 QDQKRSDLDEQLKEYINEWRKQRAKEEDELKRLIEKQAKRKVSXXXXXXXXXXXXXXXXX 478 +++ + + +E+ K E K+ A E++ KR E++AKR+ Sbjct: 582 EEKAKREAEEKAKREAEENAKREA--EEKAKREAEEKAKREAEEKAKREAEEKAKREAEE 639 Query: 479 XXXXXXXXXXXXDIEEKRQRLEEAEKKRQAMLQAMKDASK 598 + EEK +R E KR+A +A ++A + Sbjct: 640 KAKREAEEKAKREAEEKAKREAEENAKREAEEKAKREAEE 679 Score = 27.5 bits (58), Expect = 1.9 Identities = 30/142 (21%), Positives = 55/142 (38%) Frame = +2 Query: 260 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKRLIEKQAKRKVSXXXX 439 K+E E + ++ KR + +E+ K E K+ A E++ KR E++AKR+ Sbjct: 586 KREAEEKAKREAEENAKR-EAEEKAKREAEEKAKREA--EEKAKREAEEKAKREAEEKAK 642 Query: 440 XXXXXXXXXXXXXXXXXXXXXXXXXDIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFPS 619 + EEK +R E KR+A + +K ++ + Sbjct: 643 REAEEKAKREAEEKAKREAEENAKREAEEKAKREAEENAKREAE-EKVKRETEENAKRKA 701 Query: 620 KRRAKTSV*AMPSWSATRPRSS 685 + K P ++ P +S Sbjct: 702 EEEGKREADKNPEIKSSAPLAS 723 >SPBPB2B2.01 |||amino acid permease, unknown 12|Schizosaccharomyces pombe|chr 2|||Manual Length = 585 Score = 29.5 bits (63), Expect = 0.48 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = -3 Query: 138 SLGTGSWTGSTATLECGAAATLVPNSCSPG 49 ++GTG W GS+ +L G AA+++ + C G Sbjct: 96 AIGTGVWVGSSKSLYRGGAASVLIDYCIVG 125 >SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces pombe|chr 3|||Manual Length = 815 Score = 28.7 bits (61), Expect = 0.84 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 2/52 (3%) Frame = +2 Query: 518 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFPS--KRRAKTSV*AMPSWSA 667 + +R R E+ + A + KDAS GP PS +R++TSV PS S+ Sbjct: 43 VHHERNRSEDILRTGGATSETTKDASAAGPVTPSFAMKRSQTSV--QPSMSS 92 >SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 266 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/59 (28%), Positives = 31/59 (52%), Gaps = 3/59 (5%) Frame = +2 Query: 260 KQEGEGDPEFIKRQDQ---KRSDLDEQLKEYINEWRKQRAKEEDELKRLIEKQAKRKVS 427 KQE + +++ D+ K + +E L++ + + + DE+K LIEKQ +K S Sbjct: 122 KQEHLDIKKRLEKSDETVCKLKEENENLQDMLRNVGNELVESRDEIKELIEKQKVQKES 180 >SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 27.5 bits (58), Expect = 1.9 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -3 Query: 138 SLGTGSWTGSTATLECGAAATLV 70 ++GTG W GS TL G AA+++ Sbjct: 96 AIGTGVWVGSKNTLREGGAASVL 118 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +2 Query: 287 FIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDE 385 FIKR DQ L+E+L + E + KE+D+ Sbjct: 1087 FIKRVDQLEEALNEKLARLVGEQMVEGDKEKDK 1119 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +2 Query: 287 FIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDE 385 FIKR DQ L+E+L + E + KE+D+ Sbjct: 1087 FIKRVDQLEEALNEKLARLVGEQMVEGDKEKDK 1119 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -2 Query: 367 PLFAPFVDVFLQLFIQVRPLLVLTLDEFWISLTLLFRSG 251 P+FA + L LF+Q+ P + + FW S+ L+ +G Sbjct: 796 PIFAYVISKCLNLFMQIDPSIGVA---FWSSMVLVVAAG 831 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 26.6 bits (56), Expect = 3.4 Identities = 10/20 (50%), Positives = 17/20 (85%) Frame = +2 Query: 521 EEKRQRLEEAEKKRQAMLQA 580 EEKR+++EE++K + +LQA Sbjct: 138 EEKRRKIEESDKWHRVLLQA 157 >SPAC22A12.14c |||BSD domain protein, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = +2 Query: 263 QEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEE 379 Q G + E D+ RSD +E E +N+ +KQ ++E+ Sbjct: 225 QSGNDEEEIFSWGDE-RSDEEESDNEQVNDEKKQSSEED 262 >SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 26.2 bits (55), Expect = 4.5 Identities = 18/51 (35%), Positives = 22/51 (43%), Gaps = 1/51 (1%) Frame = -1 Query: 185 CWLFIECRTGRSRSLSHSALVLGPAAQRRWNV-EPRRPSCRIPAARGTTSI 36 CW I G S L+ AL+L PA+ NV S +P G SI Sbjct: 292 CWWIIPMALGSSAGLACRALLLNPASVTYPNVLSSVEISSGLPVIYGMASI 342 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +2 Query: 284 EFIKRQDQKRSDLDEQLKEYINEWRKQRAK 373 E +KR+DQK+ D+ E L+EY + +R K Sbjct: 272 ELLKRRDQKQQDV-EALQEYSAKLSLERDK 300 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +3 Query: 15 SPRWRPLYTSGPPGCRNSARGSPRLHIP 98 SPR P+ SG P N+A G P L +P Sbjct: 1589 SPRMGPVNNSGSPLAMNAA-GQPSLAVP 1615 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 1/39 (2%) Frame = +2 Query: 293 KRQDQKRSDLDEQL-KEYINEWRKQRAKEEDELKRLIEK 406 KR+ Q+ D L KEY + +++ K+ D+L+ L+ K Sbjct: 326 KREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSK 364 >SPBC365.07c |||TATA element modulatory factor homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = +2 Query: 299 QDQKRSDLDEQLKEYINEWRKQRAKEEDELKRLIEKQA 412 + Q S + +L I + +K ++ E +LKRL EKQA Sbjct: 51 EGQALSKNELKLNNTIKQLKKSLSEAETKLKRLDEKQA 88 >SPCC188.09c |||glycoprotein |Schizosaccharomyces pombe|chr 3|||Manual Length = 609 Score = 25.8 bits (54), Expect = 5.9 Identities = 15/47 (31%), Positives = 23/47 (48%) Frame = +2 Query: 53 GLQEFGTRVAAAPHSNVAVLPVQEPVPSD*VTDFYPCDTQ*TTNMSD 193 G +EF T +A+A S + V EP T Y D + TT +++ Sbjct: 192 GSEEFTTTLASASGSISGTVEVIEPTAGTVTTTIYSGDQEYTTILAE 238 >SPAC4F8.04 |||Brix domain protein Rpf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 306 Score = 25.4 bits (53), Expect = 7.9 Identities = 18/39 (46%), Positives = 24/39 (61%), Gaps = 3/39 (7%) Frame = +2 Query: 290 IKRQDQKRSDLDEQLKEYINEWRKQRAKEED---ELKRL 397 I RQ Q L ++ K+ + E RK+RAKEE+ E KRL Sbjct: 8 IVRQQQYMKALHQKNKDKL-ERRKERAKEEEKDPEKKRL 45 >SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 833 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/24 (41%), Positives = 19/24 (79%) Frame = +2 Query: 350 EWRKQRAKEEDELKRLIEKQAKRK 421 E + Q+ ++E+EL+ L E++AK+K Sbjct: 103 ELKLQKKEKEEELRNLEEEKAKKK 126 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.4 bits (53), Expect = 7.9 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +2 Query: 254 APKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKRLIEK 406 AP + E +P+ + L E+ K + E RKQR +D + IEK Sbjct: 794 APAPQAEPEPKI------DETSLTEEQKIQLEEARKQRKAADDAARAAIEK 838 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,134,313 Number of Sequences: 5004 Number of extensions: 35086 Number of successful extensions: 206 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 190 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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