SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31205
         (566 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC839.13c |rpl1601||60S ribosomal protein L13/L16|Schizosaccha...   191   5e-50
SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein L13/L16|Schiz...   189   3e-49
SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizos...   182   4e-47
SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr...    31   0.12 
SPBC16G5.04 |mrpl23||mitochondrial ribosomal protein subunit L13...    30   0.27 
SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po...    28   0.83 
SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schi...    27   1.5  
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb...    27   1.9  
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho...    26   4.4  
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha...    25   5.9  
SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual     25   7.7  

>SPBC839.13c |rpl1601||60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 197

 Score =  191 bits (466), Expect = 5e-50
 Identities = 92/175 (52%), Positives = 116/175 (66%)
 Frame = +1

Query: 1   LLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKXCNVNPARGPFHFRAPSKILWKTVRG 180
           LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG FHFRAPS+I  K VRG
Sbjct: 28  LLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAFHFRAPSRIFQKAVRG 87

Query: 181 MIPHKTERGKNALKRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIG 360
           M+PHKT RG+ AL+ L+  +G PPPFD               LKPGR YC VGRLS E+G
Sbjct: 88  MLPHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRLSSEVG 147

Query: 361 WKYRDVVRKLEDKRKGKAVKRVAYEKKLKRITKDAGEKVSKATTPFTTIIQSYGY 525
           WKY D+V KLE++RK K+     Y+ KL +  K A    +K  +P    +  +GY
Sbjct: 148 WKYNDIVAKLEERRKVKSA--AFYQAKLAKQKKIAS---AKEASPVNQKLSQFGY 197


>SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 197

 Score =  189 bits (460), Expect = 3e-49
 Identities = 89/162 (54%), Positives = 111/162 (68%)
 Frame = +1

Query: 1   LLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKXCNVNPARGPFHFRAPSKILWKTVRG 180
           LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG FHFRAPS+I  K VRG
Sbjct: 28  LLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAFHFRAPSRIFQKAVRG 87

Query: 181 MIPHKTERGKNALKRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIG 360
           M+PHKT RG+ AL+ L+  +G PPPFD               LKPGR YC VGRLS E+G
Sbjct: 88  MLPHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRLSSEVG 147

Query: 361 WKYRDVVRKLEDKRKGKAVKRVAYEKKLKRITKDAGEKVSKA 486
           WKY D+V KLE++RK K+     Y+ KL +  K A  K + +
Sbjct: 148 WKYSDIVSKLEERRKVKSA--AFYQAKLAKQKKIASAKAASS 187


>SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein
           L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 197

 Score =  182 bits (442), Expect = 4e-47
 Identities = 81/138 (58%), Positives = 100/138 (72%)
 Frame = +1

Query: 1   LLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKXCNVNPARGPFHFRAPSKILWKTVRG 180
           LL G KVVVVRCE++NISG+FFRNKLK +++LRK C  NP+RG FHFRAPS+I  K VRG
Sbjct: 28  LLAGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRYNPSRGAFHFRAPSRIFTKAVRG 87

Query: 181 MIPHKTERGKNALKRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRLSHEIG 360
           M+PHKT RG  ALK L+  +G PPPFD               LKP R YC +GRLS E+G
Sbjct: 88  MLPHKTTRGNIALKNLQALEGIPPPFDKQKRLVVPAALRVLRLKPSRKYCTIGRLSSEVG 147

Query: 361 WKYRDVVRKLEDKRKGKA 414
           WKY+++V KLE++RK K+
Sbjct: 148 WKYKNIVSKLEERRKIKS 165


>SPAPYUK71.03c |||C2 domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1225

 Score = 31.1 bits (67), Expect = 0.12
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +1

Query: 403 KGKAVKRVAYEKKLKRITKDAGEKVSKATTP 495
           KGK V R+ Y+ K   +  D+GE+++K   P
Sbjct: 540 KGKTVGRLNYDMKFHAVVPDSGEEITKVDGP 570


>SPBC16G5.04 |mrpl23||mitochondrial ribosomal protein subunit
           L13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 157

 Score = 29.9 bits (64), Expect = 0.27
 Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 2/85 (2%)
 Frame = +1

Query: 10  GNKVVVVRCEQINISGNFFRNKLKLMSFLRKXCNVN--PARGPFHFRAPSKILWKTVRGM 183
           G+ VVV  C +I ISG    N  K  S   +  ++           R    +L + V GM
Sbjct: 52  GDVVVVTDCSEIGISGRKLENH-KYYSHSGQPGHLKEWTMEEMAAKRGHKDLLRRAVGGM 110

Query: 184 IPHKTERGKNALKRLRTYDGCPPPF 258
           +P + +     +KRL  YDG   P+
Sbjct: 111 LP-RNKLWDRRMKRLYIYDGAEHPY 134


>SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 474

 Score = 28.3 bits (60), Expect = 0.83
 Identities = 17/45 (37%), Positives = 23/45 (51%)
 Frame = +1

Query: 340 RLSHEIGWKYRDVVRKLEDKRKGKAVKRVAYEKKLKRITKDAGEK 474
           R S E   K  D   K E+KR+ K  K+   EKK K+  K+  +K
Sbjct: 428 RKSVESSEKDEDEAAKKEEKRRKKEAKKEKKEKKEKKEKKEKKKK 472


>SPAC29B12.02c |set2||histone lysine methyltransferase Set2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 798

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 16/40 (40%), Positives = 24/40 (60%)
 Frame = +1

Query: 382 RKLEDKRKGKAVKRVAYEKKLKRITKDAGEKVSKATTPFT 501
           R  + KR+ K  +R+AYE+ LKR  K   EK +K++   T
Sbjct: 647 RIAQQKREEK--RRLAYEESLKRHAKKLHEKKTKSSQDAT 684


>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1919

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
 Frame = +1

Query: 394 DKRKGKAVKRVAYEKKLKRITKDAGE-KVSKATTPFTTIIQSYG 522
           DKR+   +  + YEK+L+ + K+  E  +     P  ++I+ YG
Sbjct: 18  DKRETIYIDLLNYEKQLRNLNKNTREDSLQTNLNPLLSLIKKYG 61


>SPBC3E7.01 |fab1|ste12,
           SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
           Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1932

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 10/24 (41%), Positives = 17/24 (70%)
 Frame = -1

Query: 248 GHPSYVRSLLRAFLPRSVLCGIMP 177
           G+ SY+ + L+AFLP++V   + P
Sbjct: 381 GNESYLSNFLKAFLPKTVCDYLFP 404


>SPCC1020.08 |||wybutosine biosynthesis protein
           Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 688

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = -3

Query: 513 LDDGSEWCCRLRHLLTSILGDPLKFLFIGNSLNSL 409
           L D  E   R+RH   S++G+P+ + +I   ++ L
Sbjct: 408 LPDRYEEASRVRHCALSLVGEPIFYPYINEFVSML 442


>SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 815

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = -1

Query: 227 SLLRAFLPRSVLCGIMPLTVFHRILDGAL 141
           S L AFLP+  LC ++PL    + L G L
Sbjct: 693 SSLFAFLPQLSLCMLIPLVFGKKNLPGTL 721


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,380,609
Number of Sequences: 5004
Number of extensions: 46936
Number of successful extensions: 133
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -