BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31197
(323 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 34 0.005
SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr 2|||Ma... 30 0.100
SPAPYUG7.02c |sin1||stress activated MAP kinase interacting prot... 29 0.17
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 28 0.30
SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 28 0.40
SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc... 27 0.93
SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 2.1
SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharom... 25 2.1
SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|c... 25 2.8
SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch... 25 2.8
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 3.7
SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc... 25 3.7
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 3.7
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 24 4.9
SPAC57A10.12c |ura3||dihydroorotate dehydrogenase Ura3|Schizosac... 24 4.9
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 24 4.9
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 24 4.9
SPBC30D10.08 |mgm101||mitochondrial nucleoid protein|Schizosacch... 24 4.9
SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 6.5
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 23 8.6
SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch... 23 8.6
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 34.3 bits (75), Expect = 0.005
Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 1/103 (0%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQKKSENFGLSNAQLE 186
RE E K +R+ EEK +R E + KR+A +A + ++ + K E + + E
Sbjct: 603 REAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAE 662
Query: 187 RNKTKGAAGRGEKNLPVHSH*AADHRGSLRS-TNSDRKARNSG 312
N + A + ++ ++ A+ + + N+ RKA G
Sbjct: 663 ENAKREAEEKAKREAEENAKREAEEKVKRETEENAKRKAEEEG 705
Score = 28.7 bits (61), Expect = 0.23
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
RE E K +R+ EEK +R E + KR+A A + ++
Sbjct: 571 REAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 607
Score = 28.7 bits (61), Expect = 0.23
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
RE E K +R+ EEK +R E KR+A +A + ++
Sbjct: 579 REAEEKAKREAEEKAKREAEENAKREAEEKAKREAEE 615
Score = 27.5 bits (58), Expect = 0.53
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
RE E + +R+ EEK +R E + KR+A +A + ++
Sbjct: 563 REAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEE 599
Score = 27.1 bits (57), Expect = 0.70
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
RE E +R+ EEK +R E + KR+A +A + ++
Sbjct: 595 REAEENAKREAEEKAKREAEEKAKREAEEKAKREAEE 631
>SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 354
Score = 29.9 bits (64), Expect = 0.100
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 43 EKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQK-KSENFGLSNAQLERNKTKGAA 210
+KR + E+E KRQA + +G +Q + QK K+ N L+ AQ R K A
Sbjct: 214 QKRAEILESEGKRQAAINVAEGDKQAEILDSEGQKIKTINSALAEAQAIREKASATA 270
>SPAPYUG7.02c |sin1||stress activated MAP kinase interacting protein
Sin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 29.1 bits (62), Expect = 0.17
Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = +3
Query: 99 HERMPARTGTQLHHPKEERKLRFEQCPAGAQQDQGSS--WKRRKKSPCSFAL 248
HER+ A G +H E K FE P + GS K+ KKSPC+F +
Sbjct: 576 HERLLAIDGEYVHIMPSESKNIFET-PKTSSIHAGSIILCKQSKKSPCNFKM 626
>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 606
Score = 28.3 bits (60), Expect = 0.30
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMK 105
+++ + KQ++++EKR E K ++ L+A K
Sbjct: 513 KIKNEAWSKQKEVKEKRNTRREKRKSKKEFLKAQK 547
>SPBC947.07 |||ribosome biogenesis protein
Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
Length = 233
Score = 27.9 bits (59), Expect = 0.40
Identities = 14/35 (40%), Positives = 25/35 (71%), Gaps = 3/35 (8%)
Frame = +1
Query: 4 VREIEYKKQRDI---EEKRQRLEEAEKKRQAMLQA 99
++ +E KK+R EEKR+++EE++K + +LQA
Sbjct: 123 LKHLEAKKRRIESMDEEKRRKIEESDKWHRVLLQA 157
>SPAC12B10.03 |||WD repeat protein, human WDR20
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 543
Score = 26.6 bits (56), Expect = 0.93
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = +2
Query: 125 DPTSPSKRR--AKTSV*AMPSWSATRPREQLEEEKKI 229
DP +P KR + ++ W PRE +EE KKI
Sbjct: 46 DPNTPVKRLFFMRINIAQFSRWDYPYPREYIEELKKI 82
>SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 233
Score = 25.4 bits (53), Expect = 2.1
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = +1
Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84
+++ +EY+ ++ ++K Q LEE EKK Q
Sbjct: 9 KLKSLEYRSKK-FDKKSQSLEEHEKKVQ 35
>SPAC1834.07 |klp3|krp1|kinesin-like protein
Klp3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 554
Score = 25.4 bits (53), Expect = 2.1
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 127 PNFTIQKKSENFGLSNAQLERN 192
P FTI++K +NF ++N ERN
Sbjct: 490 PGFTIEQKDKNFSINN---ERN 508
>SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 262
Score = 25.0 bits (52), Expect = 2.8
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 216 SSSCSLGLVALQLGIAQTEVFALLLDGEVGSRSLLAS 106
S SC L + + A + FAL+ E+G RS +A+
Sbjct: 163 SMSCFKNLFSKKFSRAFIKAFALIFVSELGDRSQIAT 199
>SPAC31G5.10 |eta2||Myb family transcriptional regulator
Eta2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.0 bits (52), Expect = 2.8
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 307 SSWPFCRSLSNEETLDGQRLNANEQ 233
S W F S +ET+D ++L NE+
Sbjct: 365 SQWRFVVGTSTQETIDRRKLWTNEE 389
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 24.6 bits (51), Expect = 3.7
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2
Query: 262 DGQRLNANEQGDFFLLFQL 206
DG + A +QGDFFLL ++
Sbjct: 1300 DGALVTAMKQGDFFLLDEI 1318
>SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 438
Score = 24.6 bits (51), Expect = 3.7
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +1
Query: 22 KKQRDIEEKRQRLEEAEKKRQAMLQ---AMKGCQQGPGPNFTIQKKSENFGLSNAQLERN 192
+K R ++ + ++L K A+LQ A++G + PG N +KK A RN
Sbjct: 362 EKTRTLQGRARKL--IGKAGNALLQQELALEGHRAKPGENPLAKKKVNKKRKERAAQWRN 419
Query: 193 KTKGAAGRGEK 225
K + G+ +K
Sbjct: 420 KKAESVGKKQK 430
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 24.6 bits (51), Expect = 3.7
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -2
Query: 241 NEQGDFFLLFQLLPWSCC 188
+EQG FF +F L+ SCC
Sbjct: 8 DEQGIFFPVFLLVGTSCC 25
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 24.2 bits (50), Expect = 4.9
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 4 VREIEYKKQRDIEEKRQRLEEAEKKRQAMLQ 96
+ EIE + +R E + +++ E EKKR Q
Sbjct: 573 LEEIEEENKRKQERELKKIREKEKKRDKKKQ 603
>SPAC57A10.12c |ura3||dihydroorotate dehydrogenase
Ura3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 443
Score = 24.2 bits (50), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 209 LEEEKKISLFIRIKPLTIEGLFVRQTPTERPGTL 310
L EE+ + +K I+G+ V T +RP TL
Sbjct: 311 LNEEELTDIADVLKKCKIDGVIVGNTTVQRPKTL 344
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 24.2 bits (50), Expect = 4.9
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -1
Query: 218 PLPAAPLVLLRSSWALLKPKFSLFFWMVKL 129
P + P L+SSW LK K L ++KL
Sbjct: 83 PFNSPPTFFLKSSWLPLKQKRVLTSSLIKL 112
>SPCC1393.02c |||non-specific DNA binding protein Spt2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 406
Score = 24.2 bits (50), Expect = 4.9
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAM 90
R + K QR+ E RQ+ EE ++++QAM
Sbjct: 44 RNRQRKLQRE-RELRQKYEEEQRRQQAM 70
>SPBC30D10.08 |mgm101||mitochondrial nucleoid
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 267
Score = 24.2 bits (50), Expect = 4.9
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 13 IEYKKQRDIEEKRQRLEEAE 72
I+Y+K ++I E ++ LEE E
Sbjct: 58 IQYQKDKNIYEPKENLEEKE 77
>SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 167
Score = 23.8 bits (49), Expect = 6.5
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +1
Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84
R++E KK++ E+K+++ E KK++
Sbjct: 139 RLKEQREKKEQKKEQKKEKKTERRKKKK 166
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 23.4 bits (48), Expect = 8.6
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +3
Query: 126 TQLHHPKEERKLRFEQCPAGAQQDQGSSWK 215
+ LHH L Q PAG ++ + +SWK
Sbjct: 100 SSLHHLGTYIDLFLIQSPAGGKKSRIASWK 129
>SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 747
Score = 23.4 bits (48), Expect = 8.6
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +1
Query: 103 KGCQQGPGPNFTIQKKSENFGLSNAQLERNK 195
K ++GP P+ KKS+ F L ++L ++
Sbjct: 92 KKVEKGPRPDIYDLKKSQQFELDTSRLSSDE 122
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.128 0.354
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,240,034
Number of Sequences: 5004
Number of extensions: 22930
Number of successful extensions: 97
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 87815546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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