SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31197
         (323 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo...    34   0.005
SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr 2|||Ma...    30   0.100
SPAPYUG7.02c |sin1||stress activated MAP kinase interacting prot...    29   0.17 
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy...    28   0.30 
SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo...    28   0.40 
SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc...    27   0.93 
SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   2.1  
SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharom...    25   2.1  
SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|c...    25   2.8  
SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch...    25   2.8  
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        25   3.7  
SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc...    25   3.7  
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ...    25   3.7  
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    24   4.9  
SPAC57A10.12c |ura3||dihydroorotate dehydrogenase Ura3|Schizosac...    24   4.9  
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy...    24   4.9  
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac...    24   4.9  
SPBC30D10.08 |mgm101||mitochondrial nucleoid protein|Schizosacch...    24   4.9  
SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    24   6.5  
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc...    23   8.6  
SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch...    23   8.6  

>SPAC17C9.03 |tif471||translation initiation factor eIF4G
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1403

 Score = 34.3 bits (75), Expect = 0.005
 Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 1/103 (0%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQKKSENFGLSNAQLE 186
           RE E K +R+ EEK +R  E + KR+A  +A +  ++        + K E    +  + E
Sbjct: 603 REAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAE 662

Query: 187 RNKTKGAAGRGEKNLPVHSH*AADHRGSLRS-TNSDRKARNSG 312
            N  + A  + ++    ++   A+ +    +  N+ RKA   G
Sbjct: 663 ENAKREAEEKAKREAEENAKREAEEKVKRETEENAKRKAEEEG 705



 Score = 28.7 bits (61), Expect = 0.23
 Identities = 14/37 (37%), Positives = 22/37 (59%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
           RE E K +R+ EEK +R  E + KR+A   A +  ++
Sbjct: 571 REAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 607



 Score = 28.7 bits (61), Expect = 0.23
 Identities = 14/37 (37%), Positives = 22/37 (59%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
           RE E K +R+ EEK +R  E   KR+A  +A +  ++
Sbjct: 579 REAEEKAKREAEEKAKREAEENAKREAEEKAKREAEE 615



 Score = 27.5 bits (58), Expect = 0.53
 Identities = 13/37 (35%), Positives = 23/37 (62%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
           RE E + +R+ EEK +R  E + KR+A  +A +  ++
Sbjct: 563 REAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEE 599



 Score = 27.1 bits (57), Expect = 0.70
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117
           RE E   +R+ EEK +R  E + KR+A  +A +  ++
Sbjct: 595 REAEENAKREAEEKAKREAEEKAKREAEEKAKREAEE 631


>SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 354

 Score = 29.9 bits (64), Expect = 0.100
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
 Frame = +1

Query: 43  EKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQK-KSENFGLSNAQLERNKTKGAA 210
           +KR  + E+E KRQA +   +G +Q    +   QK K+ N  L+ AQ  R K    A
Sbjct: 214 QKRAEILESEGKRQAAINVAEGDKQAEILDSEGQKIKTINSALAEAQAIREKASATA 270


>SPAPYUG7.02c |sin1||stress activated MAP kinase interacting protein
           Sin1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 665

 Score = 29.1 bits (62), Expect = 0.17
 Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
 Frame = +3

Query: 99  HERMPARTGTQLHHPKEERKLRFEQCPAGAQQDQGSS--WKRRKKSPCSFAL 248
           HER+ A  G  +H    E K  FE  P  +    GS    K+ KKSPC+F +
Sbjct: 576 HERLLAIDGEYVHIMPSESKNIFET-PKTSSIHAGSIILCKQSKKSPCNFKM 626


>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 606

 Score = 28.3 bits (60), Expect = 0.30
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +1

Query: 1   RVREIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMK 105
           +++   + KQ++++EKR    E  K ++  L+A K
Sbjct: 513 KIKNEAWSKQKEVKEKRNTRREKRKSKKEFLKAQK 547


>SPBC947.07 |||ribosome biogenesis protein
           Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 233

 Score = 27.9 bits (59), Expect = 0.40
 Identities = 14/35 (40%), Positives = 25/35 (71%), Gaps = 3/35 (8%)
 Frame = +1

Query: 4   VREIEYKKQRDI---EEKRQRLEEAEKKRQAMLQA 99
           ++ +E KK+R     EEKR+++EE++K  + +LQA
Sbjct: 123 LKHLEAKKRRIESMDEEKRRKIEESDKWHRVLLQA 157


>SPAC12B10.03 |||WD repeat protein, human WDR20
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 26.6 bits (56), Expect = 0.93
 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
 Frame = +2

Query: 125 DPTSPSKRR--AKTSV*AMPSWSATRPREQLEEEKKI 229
           DP +P KR    + ++     W    PRE +EE KKI
Sbjct: 46  DPNTPVKRLFFMRINIAQFSRWDYPYPREYIEELKKI 82


>SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
          2|||Manual
          Length = 233

 Score = 25.4 bits (53), Expect = 2.1
 Identities = 11/28 (39%), Positives = 20/28 (71%)
 Frame = +1

Query: 1  RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84
          +++ +EY+ ++  ++K Q LEE EKK Q
Sbjct: 9  KLKSLEYRSKK-FDKKSQSLEEHEKKVQ 35


>SPAC1834.07 |klp3|krp1|kinesin-like protein
           Klp3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 554

 Score = 25.4 bits (53), Expect = 2.1
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = +1

Query: 127 PNFTIQKKSENFGLSNAQLERN 192
           P FTI++K +NF ++N   ERN
Sbjct: 490 PGFTIEQKDKNFSINN---ERN 508


>SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 262

 Score = 25.0 bits (52), Expect = 2.8
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -3

Query: 216 SSSCSLGLVALQLGIAQTEVFALLLDGEVGSRSLLAS 106
           S SC   L + +   A  + FAL+   E+G RS +A+
Sbjct: 163 SMSCFKNLFSKKFSRAFIKAFALIFVSELGDRSQIAT 199


>SPAC31G5.10 |eta2||Myb family transcriptional regulator
           Eta2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 25.0 bits (52), Expect = 2.8
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -2

Query: 307 SSWPFCRSLSNEETLDGQRLNANEQ 233
           S W F    S +ET+D ++L  NE+
Sbjct: 365 SQWRFVVGTSTQETIDRRKLWTNEE 389


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 24.6 bits (51), Expect = 3.7
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = -2

Query: 262  DGQRLNANEQGDFFLLFQL 206
            DG  + A +QGDFFLL ++
Sbjct: 1300 DGALVTAMKQGDFFLLDEI 1318


>SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 438

 Score = 24.6 bits (51), Expect = 3.7
 Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
 Frame = +1

Query: 22  KKQRDIEEKRQRLEEAEKKRQAMLQ---AMKGCQQGPGPNFTIQKKSENFGLSNAQLERN 192
           +K R ++ + ++L    K   A+LQ   A++G +  PG N   +KK        A   RN
Sbjct: 362 EKTRTLQGRARKL--IGKAGNALLQQELALEGHRAKPGENPLAKKKVNKKRKERAAQWRN 419

Query: 193 KTKGAAGRGEK 225
           K   + G+ +K
Sbjct: 420 KKAESVGKKQK 430


>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
           Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 24.6 bits (51), Expect = 3.7
 Identities = 10/18 (55%), Positives = 13/18 (72%)
 Frame = -2

Query: 241 NEQGDFFLLFQLLPWSCC 188
           +EQG FF +F L+  SCC
Sbjct: 8   DEQGIFFPVFLLVGTSCC 25


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = +1

Query: 4   VREIEYKKQRDIEEKRQRLEEAEKKRQAMLQ 96
           + EIE + +R  E + +++ E EKKR    Q
Sbjct: 573 LEEIEEENKRKQERELKKIREKEKKRDKKKQ 603


>SPAC57A10.12c |ura3||dihydroorotate dehydrogenase
           Ura3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 443

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +2

Query: 209 LEEEKKISLFIRIKPLTIEGLFVRQTPTERPGTL 310
           L EE+   +   +K   I+G+ V  T  +RP TL
Sbjct: 311 LNEEELTDIADVLKKCKIDGVIVGNTTVQRPKTL 344


>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 435

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = -1

Query: 218 PLPAAPLVLLRSSWALLKPKFSLFFWMVKL 129
           P  + P   L+SSW  LK K  L   ++KL
Sbjct: 83  PFNSPPTFFLKSSWLPLKQKRVLTSSLIKL 112


>SPCC1393.02c |||non-specific DNA binding protein Spt2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 406

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 12/28 (42%), Positives = 19/28 (67%)
 Frame = +1

Query: 7   REIEYKKQRDIEEKRQRLEEAEKKRQAM 90
           R  + K QR+  E RQ+ EE ++++QAM
Sbjct: 44  RNRQRKLQRE-RELRQKYEEEQRRQQAM 70


>SPBC30D10.08 |mgm101||mitochondrial nucleoid
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 267

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +1

Query: 13  IEYKKQRDIEEKRQRLEEAE 72
           I+Y+K ++I E ++ LEE E
Sbjct: 58  IQYQKDKNIYEPKENLEEKE 77


>SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 167

 Score = 23.8 bits (49), Expect = 6.5
 Identities = 9/28 (32%), Positives = 19/28 (67%)
 Frame = +1

Query: 1   RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84
           R++E   KK++  E+K+++  E  KK++
Sbjct: 139 RLKEQREKKEQKKEQKKEKKTERRKKKK 166


>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 23.4 bits (48), Expect = 8.6
 Identities = 11/30 (36%), Positives = 16/30 (53%)
 Frame = +3

Query: 126 TQLHHPKEERKLRFEQCPAGAQQDQGSSWK 215
           + LHH      L   Q PAG ++ + +SWK
Sbjct: 100 SSLHHLGTYIDLFLIQSPAGGKKSRIASWK 129


>SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 747

 Score = 23.4 bits (48), Expect = 8.6
 Identities = 10/31 (32%), Positives = 18/31 (58%)
 Frame = +1

Query: 103 KGCQQGPGPNFTIQKKSENFGLSNAQLERNK 195
           K  ++GP P+    KKS+ F L  ++L  ++
Sbjct: 92  KKVEKGPRPDIYDLKKSQQFELDTSRLSSDE 122


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.314    0.128    0.354 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,240,034
Number of Sequences: 5004
Number of extensions: 22930
Number of successful extensions: 97
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 87815546
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)

- SilkBase 1999-2023 -