BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31197 (323 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 34 0.005 SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr 2|||Ma... 30 0.100 SPAPYUG7.02c |sin1||stress activated MAP kinase interacting prot... 29 0.17 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 28 0.30 SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 28 0.40 SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc... 27 0.93 SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 2.1 SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharom... 25 2.1 SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|c... 25 2.8 SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch... 25 2.8 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 3.7 SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc... 25 3.7 SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 3.7 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 24 4.9 SPAC57A10.12c |ura3||dihydroorotate dehydrogenase Ura3|Schizosac... 24 4.9 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 24 4.9 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 24 4.9 SPBC30D10.08 |mgm101||mitochondrial nucleoid protein|Schizosacch... 24 4.9 SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 6.5 SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 23 8.6 SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch... 23 8.6 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 34.3 bits (75), Expect = 0.005 Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 1/103 (0%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQKKSENFGLSNAQLE 186 RE E K +R+ EEK +R E + KR+A +A + ++ + K E + + E Sbjct: 603 REAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAE 662 Query: 187 RNKTKGAAGRGEKNLPVHSH*AADHRGSLRS-TNSDRKARNSG 312 N + A + ++ ++ A+ + + N+ RKA G Sbjct: 663 ENAKREAEEKAKREAEENAKREAEEKVKRETEENAKRKAEEEG 705 Score = 28.7 bits (61), Expect = 0.23 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117 RE E K +R+ EEK +R E + KR+A A + ++ Sbjct: 571 REAEEKAKREAEEKAKREAEEKAKREAEENAKREAEE 607 Score = 28.7 bits (61), Expect = 0.23 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117 RE E K +R+ EEK +R E KR+A +A + ++ Sbjct: 579 REAEEKAKREAEEKAKREAEENAKREAEEKAKREAEE 615 Score = 27.5 bits (58), Expect = 0.53 Identities = 13/37 (35%), Positives = 23/37 (62%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117 RE E + +R+ EEK +R E + KR+A +A + ++ Sbjct: 563 REAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEE 599 Score = 27.1 bits (57), Expect = 0.70 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMKGCQQ 117 RE E +R+ EEK +R E + KR+A +A + ++ Sbjct: 595 REAEENAKREAEEKAKREAEEKAKREAEEKAKREAEE 631 >SPBC16G5.07c |||prohibitin |Schizosaccharomyces pombe|chr 2|||Manual Length = 354 Score = 29.9 bits (64), Expect = 0.100 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = +1 Query: 43 EKRQRLEEAEKKRQAMLQAMKGCQQGPGPNFTIQK-KSENFGLSNAQLERNKTKGAA 210 +KR + E+E KRQA + +G +Q + QK K+ N L+ AQ R K A Sbjct: 214 QKRAEILESEGKRQAAINVAEGDKQAEILDSEGQKIKTINSALAEAQAIREKASATA 270 >SPAPYUG7.02c |sin1||stress activated MAP kinase interacting protein Sin1|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 29.1 bits (62), Expect = 0.17 Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 2/52 (3%) Frame = +3 Query: 99 HERMPARTGTQLHHPKEERKLRFEQCPAGAQQDQGSS--WKRRKKSPCSFAL 248 HER+ A G +H E K FE P + GS K+ KKSPC+F + Sbjct: 576 HERLLAIDGEYVHIMPSESKNIFET-PKTSSIHAGSIILCKQSKKSPCNFKM 626 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 28.3 bits (60), Expect = 0.30 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +1 Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQAMLQAMK 105 +++ + KQ++++EKR E K ++ L+A K Sbjct: 513 KIKNEAWSKQKEVKEKRNTRREKRKSKKEFLKAQK 547 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 27.9 bits (59), Expect = 0.40 Identities = 14/35 (40%), Positives = 25/35 (71%), Gaps = 3/35 (8%) Frame = +1 Query: 4 VREIEYKKQRDI---EEKRQRLEEAEKKRQAMLQA 99 ++ +E KK+R EEKR+++EE++K + +LQA Sbjct: 123 LKHLEAKKRRIESMDEEKRRKIEESDKWHRVLLQA 157 >SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 26.6 bits (56), Expect = 0.93 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Frame = +2 Query: 125 DPTSPSKRR--AKTSV*AMPSWSATRPREQLEEEKKI 229 DP +P KR + ++ W PRE +EE KKI Sbjct: 46 DPNTPVKRLFFMRINIAQFSRWDYPYPREYIEELKKI 82 >SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 25.4 bits (53), Expect = 2.1 Identities = 11/28 (39%), Positives = 20/28 (71%) Frame = +1 Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84 +++ +EY+ ++ ++K Q LEE EKK Q Sbjct: 9 KLKSLEYRSKK-FDKKSQSLEEHEKKVQ 35 >SPAC1834.07 |klp3|krp1|kinesin-like protein Klp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 554 Score = 25.4 bits (53), Expect = 2.1 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +1 Query: 127 PNFTIQKKSENFGLSNAQLERN 192 P FTI++K +NF ++N ERN Sbjct: 490 PGFTIEQKDKNFSINN---ERN 508 >SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 262 Score = 25.0 bits (52), Expect = 2.8 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -3 Query: 216 SSSCSLGLVALQLGIAQTEVFALLLDGEVGSRSLLAS 106 S SC L + + A + FAL+ E+G RS +A+ Sbjct: 163 SMSCFKNLFSKKFSRAFIKAFALIFVSELGDRSQIAT 199 >SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.0 bits (52), Expect = 2.8 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = -2 Query: 307 SSWPFCRSLSNEETLDGQRLNANEQ 233 S W F S +ET+D ++L NE+ Sbjct: 365 SQWRFVVGTSTQETIDRRKLWTNEE 389 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 24.6 bits (51), Expect = 3.7 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -2 Query: 262 DGQRLNANEQGDFFLLFQL 206 DG + A +QGDFFLL ++ Sbjct: 1300 DGALVTAMKQGDFFLLDEI 1318 >SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 24.6 bits (51), Expect = 3.7 Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 3/71 (4%) Frame = +1 Query: 22 KKQRDIEEKRQRLEEAEKKRQAMLQ---AMKGCQQGPGPNFTIQKKSENFGLSNAQLERN 192 +K R ++ + ++L K A+LQ A++G + PG N +KK A RN Sbjct: 362 EKTRTLQGRARKL--IGKAGNALLQQELALEGHRAKPGENPLAKKKVNKKRKERAAQWRN 419 Query: 193 KTKGAAGRGEK 225 K + G+ +K Sbjct: 420 KKAESVGKKQK 430 >SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit Sec63 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 24.6 bits (51), Expect = 3.7 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = -2 Query: 241 NEQGDFFLLFQLLPWSCC 188 +EQG FF +F L+ SCC Sbjct: 8 DEQGIFFPVFLLVGTSCC 25 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 24.2 bits (50), Expect = 4.9 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +1 Query: 4 VREIEYKKQRDIEEKRQRLEEAEKKRQAMLQ 96 + EIE + +R E + +++ E EKKR Q Sbjct: 573 LEEIEEENKRKQERELKKIREKEKKRDKKKQ 603 >SPAC57A10.12c |ura3||dihydroorotate dehydrogenase Ura3|Schizosaccharomyces pombe|chr 1|||Manual Length = 443 Score = 24.2 bits (50), Expect = 4.9 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 209 LEEEKKISLFIRIKPLTIEGLFVRQTPTERPGTL 310 L EE+ + +K I+G+ V T +RP TL Sbjct: 311 LNEEELTDIADVLKKCKIDGVIVGNTTVQRPKTL 344 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 24.2 bits (50), Expect = 4.9 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = -1 Query: 218 PLPAAPLVLLRSSWALLKPKFSLFFWMVKL 129 P + P L+SSW LK K L ++KL Sbjct: 83 PFNSPPTFFLKSSWLPLKQKRVLTSSLIKL 112 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 24.2 bits (50), Expect = 4.9 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +1 Query: 7 REIEYKKQRDIEEKRQRLEEAEKKRQAM 90 R + K QR+ E RQ+ EE ++++QAM Sbjct: 44 RNRQRKLQRE-RELRQKYEEEQRRQQAM 70 >SPBC30D10.08 |mgm101||mitochondrial nucleoid protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 267 Score = 24.2 bits (50), Expect = 4.9 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = +1 Query: 13 IEYKKQRDIEEKRQRLEEAE 72 I+Y+K ++I E ++ LEE E Sbjct: 58 IQYQKDKNIYEPKENLEEKE 77 >SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 167 Score = 23.8 bits (49), Expect = 6.5 Identities = 9/28 (32%), Positives = 19/28 (67%) Frame = +1 Query: 1 RVREIEYKKQRDIEEKRQRLEEAEKKRQ 84 R++E KK++ E+K+++ E KK++ Sbjct: 139 RLKEQREKKEQKKEQKKEKKTERRKKKK 166 >SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 23.4 bits (48), Expect = 8.6 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = +3 Query: 126 TQLHHPKEERKLRFEQCPAGAQQDQGSSWK 215 + LHH L Q PAG ++ + +SWK Sbjct: 100 SSLHHLGTYIDLFLIQSPAGGKKSRIASWK 129 >SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 747 Score = 23.4 bits (48), Expect = 8.6 Identities = 10/31 (32%), Positives = 18/31 (58%) Frame = +1 Query: 103 KGCQQGPGPNFTIQKKSENFGLSNAQLERNK 195 K ++GP P+ KKS+ F L ++L ++ Sbjct: 92 KKVEKGPRPDIYDLKKSQQFELDTSRLSSDE 122 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.314 0.128 0.354 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,240,034 Number of Sequences: 5004 Number of extensions: 22930 Number of successful extensions: 97 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 85 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 91 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 87815546 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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