Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31167
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos... 33 0.17
At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative / phos... 33 0.23
At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phos... 31 0.53
At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative / phos... 31 0.93
At1g75370.1 68414.m08754 SEC14 cytosolic factor, putative / phos... 31 0.93
At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative / phos... 30 1.2
At1g27260.1 68414.m03321 paired amphipathic helix repeat-contain... 30 1.6
At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative / phos... 29 2.2
At5g38350.1 68418.m04628 disease resistance protein (NBS-LRR cla... 29 3.8
At2g18180.1 68415.m02115 SEC14 cytosolic factor, putative / phos... 29 3.8
At3g06410.1 68416.m00739 zinc finger (CCCH-type) family protein ... 28 5.0
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 28 6.6
At5g28930.1 68418.m03578 hypothetical protein various predicted ... 27 8.7
At5g02430.1 68418.m00167 WD-40 repeat family protein contains 6 ... 27 8.7
At2g32660.1 68415.m03992 disease resistance family protein / LRR... 27 8.7
>At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
SP:P24859 from [Kluyveromyces lactissimilar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus
Length = 608
Score = 33.1 bits (72), Expect = 0.17
Identities = 25/96 (26%), Positives = 42/96 (43%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD + + NF +I+ Q + +D Y + ++II L+ K +
Sbjct: 223 ILDVQGLGLKNFTKTARDLII-QLQKIDSDNYPETLHRMFIINAGSGFKLLWGTVKSFLD 281
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K S++HV +K + E +D LP +GG T
Sbjct: 282 PKTVSKIHVLGNKYQNKLLEMIDASQLPDFFGGTCT 317
>At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar to SEC14
CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/
PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) GB:P46250 from
[Candida albicans] (Yeast (1996) 12(11), 1097-1105);
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 579
Score = 32.7 bits (71), Expect = 0.23
Identities = 26/94 (27%), Positives = 41/94 (43%), Gaps = 2/94 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
I+D V+ M+F +++R + D Y + +YII L+ N K +
Sbjct: 222 IIDVHGVSWMSFRKLAQDLVMRMQ-KIDGDNYPETLNQMYIINAGNGFKLVWNTVKGFLD 280
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGN 645
K S++HV +K E +D LP GGN
Sbjct: 281 PKTTSKIHVLGNKYRSHLLEIIDPSELPEFLGGN 314
>At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650
: CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus
Length = 548
Score = 31.5 bits (68), Expect = 0.53
Identities = 25/96 (26%), Positives = 45/96 (46%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V + +F +K +L++ + +D Y + ++II L+ + K +
Sbjct: 214 ILDVQGVGLKSF-SKAARDLLQRIQKIDSDNYPETLNRMFIINAGSGFRLLWSTVKSFLD 272
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K +++HV +K E +D + LP GGN T
Sbjct: 273 PKTTAKIHVLGNKYQSKLLEIIDSNELPEFLGGNCT 308
>At2g21520.1 68415.m02561 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to phosphatidylinositol
transfer-like protein IV (GI:14486707) [Lotus japonicus]
Length = 631
Score = 30.7 bits (66), Expect = 0.93
Identities = 25/93 (26%), Positives = 41/93 (44%), Gaps = 2/93 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V + NF +I R + D Y + ++II L+ N K +
Sbjct: 233 ILDVQGVGLKNFNKSARDLITRLQ-KIDGDNYPETLHQMFIINAGPGFRLLWNTVKSFLD 291
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGG 642
K ++++HV +K + E +D + LP GG
Sbjct: 292 PKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGG 324
>At1g75370.1 68414.m08754 SEC14 cytosolic factor, putative /
phosphatidylinositol transfer-like protein, putative
similar to phosphatidylinositol transfer-like protein
III (GI:14486705) [Lotus japonicus]; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminu
Length = 612
Score = 30.7 bits (66), Expect = 0.93
Identities = 26/96 (27%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V NF +I+ Q + D Y + ++II L+ KQ +
Sbjct: 232 ILDVQGVGFKNFSKPARDLII-QLQKIDNDNYPETLHRMFIINGGSGFKLVWATVKQFLD 290
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K +++HV +K + E +D LP GG T
Sbjct: 291 PKTVTKIHVIGNKYQNKLLEIIDASQLPDFLGGTCT 326
>At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 554
Score = 30.3 bits (65), Expect = 1.2
Identities = 26/96 (27%), Positives = 41/96 (42%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V + NF K +L+ + D Y + ++II L+ N K +
Sbjct: 215 ILDVQGVGLSNF-NKAAKDLLQSIQKIDNDNYPETLNRMFIINAGCGFRLLWNTVKSFLD 273
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K +++HV +K E +D + LP GG T
Sbjct: 274 PKTTAKIHVLGNKYQTKLLEIIDANELPEFLGGKCT 309
>At1g27260.1 68414.m03321 paired amphipathic helix repeat-containing
protein low similarity to transcriptional repressor
SIN3B [Mus musculus] GI:2921547; contains Pfam profile
PF02671: Paired amphipathic helix repeat
Length = 222
Score = 29.9 bits (64), Expect = 1.6
Identities = 20/63 (31%), Positives = 31/63 (49%)
Frame = +3
Query: 90 GLVERAKTRIDNLCTFKTLMPNFFEDIDVKKFAFSTVFSHAHMPKLTKDN*PNIYIKSLQ 269
GLVE K +D L F L+P F+ I + F V + P+ T ++ Y+ S++
Sbjct: 45 GLVELIKDHLDLLLGFNALLPARFQ-IPITPAGFQNVVGRSVPPETTIED-ATSYLNSVK 102
Query: 270 R*F 278
R F
Sbjct: 103 RAF 105
>At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus; supporting cDNA gi|23463078|gb|BT000834.1|
Length = 543
Score = 29.5 bits (63), Expect = 2.2
Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V + NF +I R + D Y + ++II ++ N K +
Sbjct: 197 ILDVQGVGLKNFNKAARDLITRLQ-KVDGDNYPETLNRMFIINAGSGFRMLWNTVKSFLD 255
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K +++HV +K E +D+ LP GG+ T
Sbjct: 256 PKTTAKIHVLGNKYQSKLLEIIDESELPEFLGGSCT 291
>At5g38350.1 68418.m04628 disease resistance protein (NBS-LRR
class), putative domain signature NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 833
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = -2
Query: 354 AVIMNFKVFAHNHNVLVIVQDEGSVKIIVVNF*YIYSVSYPWSVSAY 214
A+ + F H +++ QD+ +K +N +IY V +P + AY
Sbjct: 149 AIAKETRWFGHGSRIIITTQDQKLLKAHGIN--HIYKVEFPSAYEAY 193
>At2g18180.1 68415.m02115 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminussimilar to phosphatidylinositol
transfer-like protein IV (GI:14486707) [Lotus
japonicus];
Length = 558
Score = 28.7 bits (61), Expect = 3.8
Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +1
Query: 370 ILDYSDVNVMNFVTKFNPVILRQAGTLITDGYGMRIKGVYII*PSKTVDLILNLFKQVVS 549
ILD V + NF +I R + D Y + ++II ++ N K +
Sbjct: 200 ILDVQGVGLKNFNKAARDLITRLQ-KVDGDNYPETLNRMFIINAGSGFRMLWNTVKSFLD 258
Query: 550 TKLASRLHV--HKTIDTGTEFLDKDLLPIEYGGNGT 651
K +++HV +K E +D LP GG+ T
Sbjct: 259 PKTTAKIHVLGNKYQSKLLEIIDASELPEFLGGSCT 294
>At3g06410.1 68416.m00739 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 437
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -3
Query: 650 VPFPPYSIGNKSLSKNSVPVS 588
+P PY IG+ SLS +S PVS
Sbjct: 371 MPVAPYPIGSSSLSGSSAPVS 391
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/45 (22%), Positives = 26/45 (57%)
Frame = +1
Query: 238 TNRIYILKVYNDDFDASLVLDYYKYIVVVSEYLKIHDYCKGFEVI 372
+++++ ++ DDF ++ D K + +E+LK+H K +++
Sbjct: 153 SSKLFFYDLHGDDFKVQVMADASKSGLDEAEFLKLHSNAKRGDIV 197
>At5g28930.1 68418.m03578 hypothetical protein various predicted
proteins, Arabidopsis thaliana
Length = 509
Score = 27.5 bits (58), Expect = 8.7
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 334 LKIHDYCKGFEVILDYSDVNVMNFVTKFNPVI-LRQAGTL 450
L IHD +GF+V D +++ + NPVI L+ + T+
Sbjct: 340 LTIHDTTRGFDVAPDRVEISGIQVHVSLNPVIQLKPSSTV 379
>At5g02430.1 68418.m00167 WD-40 repeat family protein contains 6
WD-40 repeats (PF00400); rab11 binding protein, Bos
taurus, EMBL:AF117897
Length = 905
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = +1
Query: 529 LFKQVVSTKLASRLHVHKTIDTGTEFLDKDLLPIEYGGNG 648
L VVS S + K +DTG EF+ K EYG NG
Sbjct: 242 LLSSVVSNTRFSAFFLIKNLDTGKEFIVK-----EYGENG 276
>At2g32660.1 68415.m03992 disease resistance family protein / LRR
family protein contains leucine rich-repeat domains
Pfam:PF00560, INTERPRO:IPR001611; similar to Cf-4
[Lycopersicon hirsutum] gi|2808683|emb|CAA05268
Length = 589
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/41 (41%), Positives = 18/41 (43%)
Frame = -3
Query: 647 PFPPYSIGNKSLSKNSVPVSIVLCTCKRLANLVLTTCLNKF 525
P PP SI N S NS I L C R + VL N F
Sbjct: 189 PNPPVSIINLSAWNNSFTGDIPLSVCNRTSLDVLDLSYNNF 229
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,475,969
Number of Sequences: 28952
Number of extensions: 323646
Number of successful extensions: 789
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 774
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 789
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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