BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31166 (359 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 1.5 SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces pom... 25 2.7 SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po... 25 2.7 SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 25 3.5 SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8 |Schizosaccha... 25 3.5 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 4.7 SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 24 6.2 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 24 6.2 SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c... 24 8.2 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 26.2 bits (55), Expect = 1.5 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = -1 Query: 338 VSSLVSEVRNGFRFAVSVRV-TVASTHSNNNVRLVVVVESLLQLTS 204 V SL ++ + R A V TVA +N+ RL VV++SLL + S Sbjct: 1288 VDSLPTDANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLSVLS 1333 >SPBC29B5.04c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 605 Score = 25.4 bits (53), Expect = 2.7 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = +3 Query: 201 ETGELKEALDDDNKPHVIVAVRGSYSYTNTDGKPETI 311 ETG E D++ V+V + + DGKP I Sbjct: 85 ETGTYPEKDDENTNLQVVVDTKAQLNVNVNDGKPNDI 121 >SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 25.4 bits (53), Expect = 2.7 Identities = 13/50 (26%), Positives = 29/50 (58%), Gaps = 1/50 (2%) Frame = -1 Query: 353 FTLSMVSSLVSEVRNGFRFAVSVRVTVASTHSNNNVRLVVVVESLL-QLT 207 F+ ++ L + +G +AVS++ + ++ N+ +L ++ SLL Q+T Sbjct: 247 FSAEFLNGLTDHMDSGIDYAVSLQKLIDASMDKNSQKLARLIFSLLYQIT 296 >SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-lysine forming] |Schizosaccharomyces pombe|chr 1|||Manual Length = 368 Score = 25.0 bits (52), Expect = 3.5 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +3 Query: 213 LKEALDDDNKPHVIVAVRGSYSYTNTDGKPETITYFADETG 335 LKE ++DN P ++ ++ Y N +G E ++ F G Sbjct: 76 LKELPENDNSPLKHTHIQFAHCYKNQEGWREVLSRFPAGNG 116 >SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 453 Score = 25.0 bits (52), Expect = 3.5 Identities = 12/52 (23%), Positives = 23/52 (44%) Frame = +3 Query: 156 AYNYNFETSNGIVRSETGELKEALDDDNKPHVIVAVRGSYSYTNTDGKPETI 311 A N + ++ +++++D +PHV+VA G + ETI Sbjct: 97 ALGANLNLKHALIVGGMDMIRQSIDLSKRPHVVVATPGRLADLIRSNGEETI 148 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 24.6 bits (51), Expect = 4.7 Identities = 17/53 (32%), Positives = 28/53 (52%), Gaps = 3/53 (5%) Frame = -1 Query: 254 NNVRLVV---VVESLLQLTSFATHDAVGSLEIVVVSSIGAGVEFRPNDLRVVL 105 +N+ LVV + S Q+TS ++ SLE ++ +GA R +D +V L Sbjct: 956 SNIALVVDELINLSSKQITSHPQSLSIASLEASLIFVLGASSIIREDDSKVTL 1008 >SPBC11G11.02c |end3||actin cortical patch component End3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 24.2 bits (50), Expect = 6.2 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = +3 Query: 156 AYNYNFETSNGIVRSETGELKEALDDDNKPHVIVA 260 A F+ NG+ ++ + EAL +K H++ A Sbjct: 65 AMKITFDLINGVYKTVPDRVPEALVSTSKKHLVAA 99 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 24.2 bits (50), Expect = 6.2 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = +3 Query: 213 LKEALDDDNKPHVIVAVRGSYSYTNTDGKPETITYFADE 329 +K +DDD+ ++ +R YS+ + PE T F D+ Sbjct: 605 VKYTIDDDSVERIMNCIRAIYSF---EELPEFQTVFLDD 640 >SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1316 Score = 23.8 bits (49), Expect = 8.2 Identities = 9/27 (33%), Positives = 14/27 (51%) Frame = +3 Query: 96 PPTEDYPKIVRSEFDASPDGAYNYNFE 176 PP +D+P + F+ + YNY E Sbjct: 1255 PPVKDFPTLEFGSFEKIYNVGYNYGKE 1281 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,368,805 Number of Sequences: 5004 Number of extensions: 23512 Number of successful extensions: 76 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 76 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 110009772 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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