BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31165
(340 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 49 2e-07
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 42 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 36 0.001
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 35 0.004
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 33 0.012
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 26 1.8
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 24 5.5
SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotran... 24 5.5
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 24 5.5
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 24 7.3
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 24 7.3
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 49.2 bits (112), Expect = 2e-07
Identities = 22/52 (42%), Positives = 31/52 (59%)
Frame = +3
Query: 174 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGENDLRATLDSLGR 329
++ AKR S F+ + Q+ E KEAF L+D D DG IG D++ L SL +
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQ 79
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 42.3 bits (95), Expect = 2e-05
Identities = 18/37 (48%), Positives = 26/37 (70%)
Frame = +3
Query: 219 SQKQVAEFKEAFQLMDHDKDGIIGENDLRATLDSLGR 329
+ +Q+AEF+EAF L D D+DG I N+L + SLG+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQ 43
Score = 27.5 bits (58), Expect = 0.59
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 237 EFKEAFQLMDHDKDGIIGENDLRATLDSLG 326
E +EAF++ D D +G I +L L SLG
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLG 115
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 36.3 bits (80), Expect = 0.001
Identities = 18/36 (50%), Positives = 24/36 (66%)
Frame = +3
Query: 219 SQKQVAEFKEAFQLMDHDKDGIIGENDLRATLDSLG 326
S++Q E KEAF L D DKDG+I + + + L SLG
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLG 39
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 34.7 bits (76), Expect = 0.004
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +3
Query: 219 SQKQVAEFKEAFQLMDHDKDGIIGENDLRATLDSLG 326
+++Q + EAF+L D DKD I ++LRA + +LG
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 33.1 bits (72), Expect = 0.012
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +3
Query: 237 EFKEAFQLMDHDKDGIIGENDLRATLDSLG 326
EF + FQ+ D D G+IG +LR L SLG
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLG 107
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.8 bits (54), Expect = 1.8
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 228 QVAEFKEAFQLMDHDKDGIIGENDLRATLDSLGRLA 335
+ A+FK+ F DKD + + +L A ++ L +LA
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLA 247
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 24.2 bits (50), Expect = 5.5
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +3
Query: 249 AFQLMDHDKDGIIGENDLRATLDSLGRL 332
AFQL D D +G+I +++ +D++ ++
Sbjct: 104 AFQLYDLDNNGLISYDEMLRIVDAIYKM 131
>SPCC417.11c |||glutamate-1-semialdehyde
2,1-aminomutaseaminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 435
Score = 24.2 bits (50), Expect = 5.5
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 155 QAILSRKPQG*AHRIQCLLYVLTEAGRRVQGGIP 256
+ + SR G +I CL LT G+ + GG+P
Sbjct: 244 EVMTSRLSPGGLQQIYCLKPDLTTLGKYLGGGLP 277
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 24.2 bits (50), Expect = 5.5
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = +3
Query: 237 EFKEAFQLMDHDKDGIIGENDLRATLDSLG 326
E +EAF L D G I DLR + LG
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLG 42
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 23.8 bits (49), Expect = 7.3
Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = +3
Query: 159 QSSRGSRKAKRTGSNVFSMFSQKQVAEFKEAFQL-MDHDKDGIIGEN 296
++ R R+ GSN M K+ E KEA + MD +K I N
Sbjct: 825 RAKRAEREKASKGSNDDEMKIDKKTTEEKEATPMEMDEEKSQDISIN 871
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 23.8 bits (49), Expect = 7.3
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +3
Query: 231 VAEFKEAFQLMDHDKDGIIGENDLRAT 311
+AEF ++H K GI G L+ T
Sbjct: 4 IAEFDGILDSLEHSKTGISGSKILKLT 30
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.122 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 861,758
Number of Sequences: 5004
Number of extensions: 9359
Number of successful extensions: 38
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 98026656
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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