BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31142 (794 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 485 e-138 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 279 3e-76 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 264 1e-71 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 175 8e-45 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 97 2e-21 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 89 9e-19 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 88 2e-18 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 80 4e-16 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 68 2e-12 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.33 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.1 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 5.4 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 485 bits (1197), Expect = e-138 Identities = 225/250 (90%), Positives = 238/250 (95%) Frame = +3 Query: 15 GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEH 194 G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEH Sbjct: 42 GIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEH 101 Query: 195 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 374 P LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+H Sbjct: 102 PCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTH 161 Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVAL 554 TVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVAL Sbjct: 162 TVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVAL 221 Query: 555 DFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNS 734 DFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQP LG+E+ GIHE YNS Sbjct: 222 DFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNS 281 Query: 735 IMKCDVDIRK 764 IMKCDVDIRK Sbjct: 282 IMKCDVDIRK 291 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 279 bits (684), Expect = 3e-76 Identities = 124/242 (51%), Positives = 177/242 (73%) Frame = +3 Query: 39 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 218 KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109 Query: 219 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGY 398 +NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169 Query: 399 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMAT 578 L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + Sbjct: 170 VLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKL 229 Query: 579 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNSIMKCDVDI 758 + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDI Sbjct: 230 SEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDI 289 Query: 759 RK 764 RK Sbjct: 290 RK 291 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 264 bits (646), Expect = 1e-71 Identities = 122/249 (48%), Positives = 172/249 (69%), Gaps = 1/249 (0%) Frame = +3 Query: 18 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 194 VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L PEEH Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 Query: 195 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 374 P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166 Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVAL 554 +VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA Sbjct: 167 SVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVAS 226 Query: 555 DFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNS 734 DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ G+H ++ S Sbjct: 227 DFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKS 286 Query: 735 IMKCDVDIR 761 I+ D+D+R Sbjct: 287 ILLSDIDLR 295 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 175 bits (425), Expect = 8e-45 Identities = 103/258 (39%), Positives = 142/258 (55%), Gaps = 16/258 (6%) Frame = +3 Query: 42 DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 218 D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 219 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 374 LNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYV 548 +P+ EGY + +I + LAGRD+T ++ +L +R S TAER IKE+ CYV Sbjct: 193 IIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYV 247 Query: 549 ALDFEQEMA--TAAASTSLEKSYELPDGQ--VITIGNERFRCPEALFQPXXLGME-SCGI 713 D +E + L+ + E G I +G ERF PE F P + + Sbjct: 248 CPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPL 307 Query: 714 HETVYNSIMKCDVDIRKG 767 E V N + +D+RKG Sbjct: 308 PELVDNVVQSSPIDVRKG 325 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 97.5 bits (232), Expect = 2e-21 Identities = 54/195 (27%), Positives = 101/195 (51%), Gaps = 3/195 (1%) Frame = +3 Query: 30 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 206 + + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ + Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120 Query: 207 TEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 380 TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH + Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180 Query: 381 PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 560 P+ G + R+ G + YL+K+ + SF + C+V+ D+ Sbjct: 181 PVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDY 240 Query: 561 EQEMATAAASTSLEK 605 ++A A LE+ Sbjct: 241 THDIAHALDRDILER 255 Score = 27.9 bits (59), Expect = 1.8 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +3 Query: 648 ERFRCPEALFQPXXLGMESCGIHETVYNSIMK 743 E+ R PE +F P +G++ GI E + SI++ Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILE-IMRSILQ 624 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 88.6 bits (210), Expect = 9e-19 Identities = 43/134 (32%), Positives = 69/134 (51%) Frame = +3 Query: 84 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 263 + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 264 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 443 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182 Query: 444 LTDYLMKILTERGY 485 + D L + L ++ + Sbjct: 183 INDILAQTLRDKNF 196 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 87.8 bits (208), Expect = 2e-18 Identities = 71/268 (26%), Positives = 118/268 (44%), Gaps = 38/268 (14%) Frame = +3 Query: 48 YVGDEAQSKRGILTLKYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEA 215 ++G+E + TL++ H G + +W +W N + P ++ +LLT+ Sbjct: 43 FLGNELANCNDFTTLQFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQP 102 Query: 216 PLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG--------------IVLD 353 + Q++FE F A A L + G T +V+D Sbjct: 103 VFTMPSIEHNTIQLVFEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVID 162 Query: 354 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 533 SG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKE 220 Query: 534 KLCYVALDFEQEMATA--AASTSLEKSYELPD------------------GQVITIGNER 653 +CYV+ +F+++M + LE Y LPD QV+ + NER Sbjct: 221 SVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNER 280 Query: 654 FRCPEALFQPXXLGMESCGIHETVYNSI 737 F PE LF P + + GI E V S+ Sbjct: 281 FMIPELLFSPSDIEIREAGIPEAVMQSV 308 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 79.8 bits (188), Expect = 4e-16 Identities = 43/161 (26%), Positives = 80/161 (49%), Gaps = 2/161 (1%) Frame = +3 Query: 15 GVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPE 188 GV + ++ YV DE Q I + +K +GII +W+ W +L+V P Sbjct: 41 GVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPT 100 Query: 189 EHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGV 368 E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D G Sbjct: 101 EYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDN 160 Query: 369 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 491 + P+ +G + I + LAG L + ++ F Sbjct: 161 ASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQLFNTMNIEF 201 Score = 51.6 bits (118), Expect = 1e-07 Identities = 33/101 (32%), Positives = 46/101 (45%), Gaps = 9/101 (8%) Frame = +3 Query: 486 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 665 S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299 Query: 666 EALFQPXXLGMESC---------GIHETVYNSIMKCDVDIR 761 E LF P S G+HE VY SI+ CD ++R Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELR 340 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 67.7 bits (158), Expect = 2e-12 Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%) Frame = +3 Query: 99 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 266 PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 267 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 446 P +A + ++ LYA G G+V+D G + PI +G + A +L L GR + Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231 Query: 447 TDYLMKILTERGYSFTTTAEREIVRDIKE 533 T +L +L E + ++ + DI E Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.9 bits (84), Expect = 0.002 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%) Frame = +3 Query: 90 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 233 L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L + Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257 Query: 234 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 413 EK+ I+F + + +++ + + +G + V+D G + + EG +P++ Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317 Query: 414 ILRLDLAGRDLTDYLMKILTERGYSF 491 ++++ G D+T MK+L + + Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.33 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +3 Query: 159 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 302 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 4.1 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +1 Query: 385 STKVTLCPTPSSVWTW 432 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 5.4 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 201 LLTEAPLNPKANREKMTQIMFETFN 275 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,878,672 Number of Sequences: 5004 Number of extensions: 54835 Number of successful extensions: 199 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 190 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 387388442 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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