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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV31142
         (794 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   485   e-138
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   279   3e-76
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   264   1e-71
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   175   8e-45
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    97   2e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    89   9e-19
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    88   2e-18
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    80   4e-16
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    68   2e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    38   0.002
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.33 
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    27   4.1  
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   5.4  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  485 bits (1197), Expect = e-138
 Identities = 225/250 (90%), Positives = 238/250 (95%)
 Frame = +3

Query: 15  GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEH 194
           G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEH
Sbjct: 42  GIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEH 101

Query: 195 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 374
           P LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+H
Sbjct: 102 PCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTH 161

Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVAL 554
           TVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVAL
Sbjct: 162 TVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVAL 221

Query: 555 DFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNS 734
           DFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQP  LG+E+ GIHE  YNS
Sbjct: 222 DFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNS 281

Query: 735 IMKCDVDIRK 764
           IMKCDVDIRK
Sbjct: 282 IMKCDVDIRK 291


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  279 bits (684), Expect = 3e-76
 Identities = 124/242 (51%), Positives = 177/242 (73%)
 Frame = +3

Query: 39  KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 218
           KD  VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P
Sbjct: 50  KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109

Query: 219 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGY 398
           +NP ANREKM + MFE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE  
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169

Query: 399 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMAT 578
            L H + RLD+AGRD T YL+ +L  +GY+F  TA+ E VR++KEKLCYV+ D E +   
Sbjct: 170 VLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKL 229

Query: 579 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNSIMKCDVDI 758
           +  +T L ++Y LPDG+VI +G+ER+ CPE LFQP  +G E  G+ E ++++I   DVDI
Sbjct: 230 SEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDI 289

Query: 759 RK 764
           RK
Sbjct: 290 RK 291


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  264 bits (646), Expect = 1e-71
 Identities = 122/249 (48%), Positives = 172/249 (69%), Gaps = 1/249 (0%)
 Frame = +3

Query: 18  VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 194
           VM    QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L   PEEH
Sbjct: 47  VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106

Query: 195 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 374
           P+LLTE PL    N+EK+ +  +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166

Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVAL 554
           +VPIY+G+++P AI + ++ GRD+TDYL   L + G+   ++AE+EIVR+IKEK CYVA 
Sbjct: 167 SVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVAS 226

Query: 555 DFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPXXLGMESCGIHETVYNS 734
           DF  E+ +         +Y+LPD Q IT+G E F  PE LF P  +G E+ G+H  ++ S
Sbjct: 227 DFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKS 286

Query: 735 IMKCDVDIR 761
           I+  D+D+R
Sbjct: 287 ILLSDIDLR 295


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  175 bits (425), Expect = 8e-45
 Identities = 103/258 (39%), Positives = 142/258 (55%), Gaps = 16/258 (6%)
 Frame = +3

Query: 42  DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 218
           D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  LLTE P
Sbjct: 73  DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132

Query: 219 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 374
           LNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DSGDGV+H
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192

Query: 375 TVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYV 548
            +P+ EGY +  +I  + LAGRD+T ++  +L +R    S   TAER     IKE+ CYV
Sbjct: 193 IIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYV 247

Query: 549 ALDFEQEMA--TAAASTSLEKSYELPDGQ--VITIGNERFRCPEALFQPXXLGME-SCGI 713
             D  +E +         L+ + E   G    I +G ERF  PE  F P     +    +
Sbjct: 248 CPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPL 307

Query: 714 HETVYNSIMKCDVDIRKG 767
            E V N +    +D+RKG
Sbjct: 308 PELVDNVVQSSPIDVRKG 325


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 97.5 bits (232), Expect = 2e-21
 Identities = 54/195 (27%), Positives = 101/195 (51%), Gaps = 3/195 (1%)
 Frame = +3

Query: 30  MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 206
           + +  + VG++   + G  ++ + P E  +I+NWD ME++  +TF  +L +   EHP+ +
Sbjct: 62  LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120

Query: 207 TEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 380
           TE   NP   R  MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+  SH +
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180

Query: 381 PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 560
           P+  G  +     R+   G   + YL+K+   +  SF           +    C+V+ D+
Sbjct: 181 PVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDY 240

Query: 561 EQEMATAAASTSLEK 605
             ++A A     LE+
Sbjct: 241 THDIAHALDRDILER 255



 Score = 27.9 bits (59), Expect = 1.8
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +3

Query: 648 ERFRCPEALFQPXXLGMESCGIHETVYNSIMK 743
           E+ R PE +F P  +G++  GI E +  SI++
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILE-IMRSILQ 624


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 88.6 bits (210), Expect = 9e-19
 Identities = 43/134 (32%), Positives = 69/134 (51%)
 Frame = +3

Query: 84  LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 263
           + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + MF
Sbjct: 63  MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122

Query: 264 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRD 443
           E+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+G+ L         +G  
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSGNA 182

Query: 444 LTDYLMKILTERGY 485
           + D L + L ++ +
Sbjct: 183 INDILAQTLRDKNF 196


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 87.8 bits (208), Expect = 2e-18
 Identities = 71/268 (26%), Positives = 118/268 (44%), Gaps = 38/268 (14%)
 Frame = +3

Query: 48  YVGDEAQSKRGILTLKYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEA 215
           ++G+E  +     TL++   H  G + +W     +W     N   + P   ++ +LLT+ 
Sbjct: 43  FLGNELANCNDFTTLQFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQP 102

Query: 216 PLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG--------------IVLD 353
                +      Q++FE F   A      A L  +  G  T               +V+D
Sbjct: 103 VFTMPSIEHNTIQLVFEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVID 162

Query: 354 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 533
           SG   +H +P+ +      A+ R+D+ G+ LT+YL ++++ R Y+     E  IV +IKE
Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKE 220

Query: 534 KLCYVALDFEQEMATA--AASTSLEKSYELPD------------------GQVITIGNER 653
            +CYV+ +F+++M        + LE  Y LPD                   QV+ + NER
Sbjct: 221 SVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNER 280

Query: 654 FRCPEALFQPXXLGMESCGIHETVYNSI 737
           F  PE LF P  + +   GI E V  S+
Sbjct: 281 FMIPELLFSPSDIEIREAGIPEAVMQSV 308


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 79.8 bits (188), Expect = 4e-16
 Identities = 43/161 (26%), Positives = 80/161 (49%), Gaps = 2/161 (1%)
 Frame = +3

Query: 15  GVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPE 188
           GV   +  ++ YV DE Q    I  + +K    +GII +W+     W      +L+V P 
Sbjct: 41  GVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPT 100

Query: 189 EHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGV 368
           E+ +++TE   NP++ R+++ +  FE  + PA Y+  QAV   +A+ ++T +++D G   
Sbjct: 101 EYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDN 160

Query: 369 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 491
           +   P+ +G  +   I +  LAG  L   + ++       F
Sbjct: 161 ASVTPVVDGLIIRKGIFKQSLAGDFLNANIEQLFNTMNIEF 201



 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 33/101 (32%), Positives = 46/101 (45%), Gaps = 9/101 (8%)
 Frame = +3

Query: 486 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 665
           S+    +R ++ + KE +  V    +     A AS+   K +E PDG     G ERFR  
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299

Query: 666 EALFQPXXLGMESC---------GIHETVYNSIMKCDVDIR 761
           E LF P      S          G+HE VY SI+ CD ++R
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELR 340


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%)
 Frame = +3

Query: 99  PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 266
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 267 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 446
               P   +A + ++ LYA G   G+V+D G   +   PI +G  +  A  +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231

Query: 447 TDYLMKILTERGYSFTTTAEREIVRDIKE 533
           T +L  +L E   +  ++ +     DI E
Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
 Frame = +3

Query: 90  LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 233
           L YPI+ G+    D         D+ +I+ ++  + L++   +   + V+     L  + 
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257

Query: 234 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 413
             EK+  I+F   +     +  +++ + + +G +   V+D G   +    + EG  +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317

Query: 414 ILRLDLAGRDLTDYLMKILTERGYSF 491
            ++++  G D+T   MK+L    + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.33
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +3

Query: 159  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 302
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 9/16 (56%), Positives = 9/16 (56%)
 Frame = +1

Query: 385 STKVTLCPTPSSVWTW 432
           STK   CP PS  W W
Sbjct: 52  STKTATCPDPSWEWAW 67


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 201 LLTEAPLNPKANREKMTQIMFETFN 275
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,878,672
Number of Sequences: 5004
Number of extensions: 54835
Number of successful extensions: 199
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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