BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31140 (453 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 279 1e-76 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 146 1e-36 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 137 6e-34 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 108 3e-25 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 81 6e-17 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 75 5e-15 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 69 3e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 61 9e-11 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 40 2e-04 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 1.3 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 4.1 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 5.4 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 7.2 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 7.2 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 7.2 SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 7.2 SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 24 9.5 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 279 bits (685), Expect = 1e-76 Identities = 130/150 (86%), Positives = 139/150 (92%) Frame = +3 Query: 3 DIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGME 182 DIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E Sbjct: 211 DIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLE 270 Query: 183 SCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKII 362 + GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+ Sbjct: 271 NAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIV 330 Query: 363 APPERKYSVWIGGSILAXLSTFQQMWISKE 452 APPERKYSVWIGGSILA LSTFQQMWISK+ Sbjct: 331 APPERKYSVWIGGSILASLSTFQQMWISKQ 360 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 146 bits (354), Expect = 1e-36 Identities = 66/150 (44%), Positives = 97/150 (64%) Frame = +3 Query: 3 DIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGME 182 +IKEK CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E Sbjct: 216 EIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSE 275 Query: 183 SCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKII 362 + G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++KI Sbjct: 276 ASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIY 335 Query: 363 APPERKYSVWIGGSILAXLSTFQQMWISKE 452 A PER ++ W+GGSILA LSTF+++ I+ E Sbjct: 336 ASPERMHNAWLGGSILASLSTFRRLLITSE 365 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 137 bits (332), Expect = 6e-34 Identities = 69/161 (42%), Positives = 105/161 (65%), Gaps = 12/161 (7%) Frame = +3 Query: 3 DIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGME 182 ++KEKLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E Sbjct: 211 EMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSE 270 Query: 183 SCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL--------AP 338 G+ E ++++I DVDIRK LY V+SGG++MY G+ R++KEI L P Sbjct: 271 QPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDP 330 Query: 339 STI---KIKIIAPPERKYSVWIGGSILA-XLSTFQQMWISK 449 + + K+KI P R+++V+IGG++LA ++ MW+SK Sbjct: 331 ARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSK 371 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 108 bits (260), Expect = 3e-25 Identities = 59/142 (41%), Positives = 79/142 (55%), Gaps = 11/142 (7%) Frame = +3 Query: 60 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 212 A AS+ K +E PDG G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 213 SIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYS 386 SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + + Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390 Query: 387 VWIGGSILAXLSTFQQMWISKE 452 VW GGSILA L FQ +W+SK+ Sbjct: 391 VWFGGSILASLDNFQHLWVSKQ 412 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 81.4 bits (192), Expect = 6e-17 Identities = 40/91 (43%), Positives = 59/91 (64%), Gaps = 3/91 (3%) Frame = +3 Query: 189 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 368 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 369 ---PERKYSVWIGGSILAXLSTFQQMWISKE 452 ER Y+ W+GGSIL+ L TF Q+WIS++ Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQ 415 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 74.9 bits (176), Expect = 5e-15 Identities = 46/144 (31%), Positives = 70/144 (48%), Gaps = 20/144 (13%) Frame = +3 Query: 3 DIKEKLCYVALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITI 122 +IKE +CYV+ +F+++M + LE Y LPD QV+ + Sbjct: 217 EIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNL 276 Query: 123 GNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIA 302 NERF PE LF PS + + GI E V S+ +I+ L N V GG +PG Sbjct: 277 SNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFH 336 Query: 303 DRMQKEITALAPSTIKIKIIAPPE 374 R+ E+ +LAP+ ++K+ P + Sbjct: 337 KRLSSELRSLAPANWEVKVFEPSD 360 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 68.9 bits (161), Expect = 3e-13 Identities = 47/160 (29%), Positives = 74/160 (46%), Gaps = 19/160 (11%) Frame = +3 Query: 6 IKEKLCYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 173 IKE+ CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299 Query: 174 GMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI- 347 + + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIH 359 Query: 348 -------------KIKIIAPPERKYSVWIGGSILAXLSTF 428 + +I+ ++ +VW GGS+LA F Sbjct: 360 RSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 60.9 bits (141), Expect = 9e-11 Identities = 27/108 (25%), Positives = 57/108 (52%) Frame = +3 Query: 129 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 308 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 309 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILAXLSTFQQMWISKE 452 +++E+T++ P I + W G S + F+ +++E Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTRE 701 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 39.5 bits (88), Expect = 2e-04 Identities = 18/70 (25%), Positives = 36/70 (51%), Gaps = 3/70 (4%) Frame = +3 Query: 243 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 413 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 414 XLSTFQQMWI 443 + ++WI Sbjct: 587 RIRIVSELWI 596 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 1.3 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 315 KEITALAPSTIKIKIIAPPERKYSVWIGGSILAXLST 425 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 4.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +2 Query: 194 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 322 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 5.4 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +2 Query: 341 DHQDQDHRSPREEVLRMDRWIHPG 412 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 24.6 bits (51), Expect = 7.2 Identities = 22/93 (23%), Positives = 37/93 (39%), Gaps = 1/93 (1%) Frame = +3 Query: 111 VITIGNERFRCPEALFQPSFLGMESC-GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 287 ++T+ NE + E L S S E+ + CD+D ++ + + T + Sbjct: 136 LVTLPNENNQVIEPLSSQSCKSQLSTQNYSESDFGWNQLCDLD---PIFKSLAFTDDTNL 192 Query: 288 YPGIADRMQKEITALAPSTIKIKIIAPPERKYS 386 +P AD I ++K A RKYS Sbjct: 193 FPAFADSEAALIMLKKREMTRVKHRAGRPRKYS 225 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 24.6 bits (51), Expect = 7.2 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -3 Query: 382 YFLSGGAMILILMVEGARAVISFCILSAIPGY 287 Y +SGGA + +A +S CI +PGY Sbjct: 412 YCISGGAAL----AASTQAFLSSCICPVLPGY 439 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 24.6 bits (51), Expect = 7.2 Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = +3 Query: 21 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 146 CYV+ +E + + SL SY +PD + I + R+ P Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81 >SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 24.6 bits (51), Expect = 7.2 Identities = 16/36 (44%), Positives = 19/36 (52%) Frame = +3 Query: 6 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 113 IKEKL VA EQE A +SL+ S + G V Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146 >SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 703 Score = 24.2 bits (50), Expect = 9.5 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +3 Query: 237 IRKDLYANTVMSGGTTMYPGIADRMQKEI 323 I LY++ S T + GI DR++K + Sbjct: 533 IYSSLYSSLNSSNSTVSFSGIFDRVEKSV 561 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,584,176 Number of Sequences: 5004 Number of extensions: 29548 Number of successful extensions: 112 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 106 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 168258430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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