BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31138 (784 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 110 2e-25 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 67 3e-12 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 49 7e-07 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 31 0.14 SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 31 0.19 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 30 0.33 SPCPB1C11.01 |amt1||ammonium transporter Amt1|Schizosaccharomyce... 28 1.3 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.3 SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 27 3.0 SPAC56F8.13 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 27 4.0 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 4.0 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 26 5.3 SPCC613.07 |||zf-HIT|Schizosaccharomyces pombe|chr 3|||Manual 26 7.0 SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual 25 9.3 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 9.3 SPCC2H8.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 25 9.3 SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 25 9.3 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 9.3 SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr 1... 25 9.3 SPAC5D6.02c |mug165||sequence orphan|Schizosaccharomyces pombe|c... 25 9.3 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 110 bits (265), Expect = 2e-25 Identities = 51/171 (29%), Positives = 85/171 (49%), Gaps = 2/171 (1%) Frame = +1 Query: 94 CNSCDKVIQ-GRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVHCVPCFTKHHSPR 270 C+SC ++ GRI++A KK HP+ F C+TC + ++ F+ +C + + SPR Sbjct: 258 CHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPR 317 Query: 271 CHGCGDPITDRVIQALGVSWHA-HHFVXXXXXXXXXXXXFMEQAGRPYCSDCYADKFATR 447 C C PI D+ + +H HHF + + +C CY +K+A + Sbjct: 318 CKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVK 377 Query: 448 CKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSVMENQPLCGKC 600 CK C PI+ ++ D ++H C+TC C + D + ++EN P+C C Sbjct: 378 CKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 66.9 bits (156), Expect = 3e-12 Identities = 45/167 (26%), Positives = 66/167 (39%), Gaps = 10/167 (5%) Frame = +1 Query: 73 KVEAPAVCNSCDKVIQGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVH-----C 237 KV + +C SC +VI G+ V AL +H E F C+ C + F + ++ C Sbjct: 109 KVSSSKICASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLC 168 Query: 238 VPCFTKHHSPRCHGCGDPITDRVIQALGVSWHAHHFV-XXXXXXXXXXXXFMEQAGRPYC 414 + + C CG + I AL +H HF + E G+ YC Sbjct: 169 ETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYC 228 Query: 415 SDCYADKFATRCKGCGNPIVDKAI----IALDAKWHRDCFTCMKCRN 543 Y+ FA RC GC PI+ + + + WH C K N Sbjct: 229 HYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPCHMIYKFWN 275 Score = 32.3 bits (70), Expect = 0.081 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +1 Query: 448 CKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSV 570 C CG I + + AL +H +CF C C + V F + Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPI 156 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 49.2 bits (112), Expect = 7e-07 Identities = 24/67 (35%), Positives = 30/67 (44%) Frame = +1 Query: 400 GRPYCSDCYADKFATRCKGCGNPIVDKAIIALDAKWHRDCFTCMKCRNPVTDSTFSVMEN 579 GRP CS C A C C I D A+++ +HR+CF C CR + DS F Sbjct: 67 GRPVCSSC-----AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNR 121 Query: 580 QPLCGKC 600 C C Sbjct: 122 TIFCNDC 128 Score = 35.9 bits (79), Expect = 0.007 Identities = 17/59 (28%), Positives = 26/59 (44%) Frame = +1 Query: 94 CNSCDKVIQGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVHCVPCFTKHHSPR 270 C +C I+ + + +H E F C+ CRK I + F + N + C C H R Sbjct: 78 CTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136 Score = 35.5 bits (78), Expect = 0.009 Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 5/64 (7%) Frame = +1 Query: 427 ADKFATRCKGCGNPIVDKAI-IALDAK-WHRDCFTCMKCRNPVTDS---TFSVMENQPLC 591 + K +T C CG + I+ WH+DCF C KC + S + +P+C Sbjct: 12 SSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVC 71 Query: 592 GKCA 603 CA Sbjct: 72 SSCA 75 Score = 28.7 bits (61), Expect = 0.99 Identities = 25/121 (20%), Positives = 40/121 (33%), Gaps = 2/121 (1%) Frame = +1 Query: 67 STKVEAPAVCNSCDKVIQGRI--VTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVHCV 240 S + VC C + Q R ++ WH + F C C K ++ + Sbjct: 10 SPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRP 69 Query: 241 PCFTKHHSPRCHGCGDPITDRVIQALGVSWHAHHFVXXXXXXXXXXXXFMEQAGRPYCSD 420 C + H+ C C I D + + S+H F F +C+D Sbjct: 70 VCSSCAHT--CTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCND 127 Query: 421 C 423 C Sbjct: 128 C 128 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 31.5 bits (68), Expect = 0.14 Identities = 32/124 (25%), Positives = 45/124 (36%), Gaps = 4/124 (3%) Frame = +1 Query: 64 MSTKVEAPAVCNSC-DKVIQGRIVTALNKKWHPEHFVCNTCRKPIDGAK--FHQHN-NGV 231 + T E C C + V V K WH + F C C K +D + F Q + + Sbjct: 14 LETPSERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQI 73 Query: 232 HCVPCFTKHHSPRCHGCGDPITDRVIQALGVSWHAHHFVXXXXXXXXXXXXFMEQAGRPY 411 C C C+GC PI + A+ S A+H F + G+ Y Sbjct: 74 FCKLCV-----DICNGCSTPICE--FNAVSNS-QANHPCCSNCRAFINSNAFGKFKGQHY 125 Query: 412 CSDC 423 C C Sbjct: 126 CLPC 129 Score = 29.5 bits (63), Expect = 0.57 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = +1 Query: 91 VCNSCDKVIQGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVHCVPCFTK 255 +CN C I + ++ HP C+ CR I+ F + +C+PC K Sbjct: 81 ICNGCSTPICEFNAVSNSQANHP---CCSNCRAFINSNAFGKFKGQHYCLPCLRK 132 Score = 28.7 bits (61), Expect = 0.99 Identities = 11/39 (28%), Positives = 17/39 (43%), Gaps = 1/39 (2%) Frame = +1 Query: 448 CKGCGNPIVDKAIIALDAK-WHRDCFTCMKCRNPVTDST 561 C C + + + K WH DCF C+ C + S+ Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSS 62 >SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 31.1 bits (67), Expect = 0.19 Identities = 13/45 (28%), Positives = 22/45 (48%) Frame = +1 Query: 214 QHNNGVHCVPCFTKHHSPRCHGCGDPITDRVIQALGVSWHAHHFV 348 Q+ + + PC + H+ P C C D + ++ I +W AH V Sbjct: 382 QYGSKIEDTPC-SLHNVPGCFSCFDRLGEKNITETNSNWFAHRLV 425 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 30.3 bits (65), Expect = 0.33 Identities = 25/93 (26%), Positives = 45/93 (48%) Frame = +2 Query: 245 ASPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTA 424 +S IT+ ++++TP + S L +T+SS +++ + N + TA Sbjct: 3368 SSTPITSSTVVNSSTPITS--STALNTSTPITSSSVLNSSTPITSSTVVNSSTPITSSTA 3425 Query: 425 TQTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 TS+P+ + S+ SS ++ S TAIAS Sbjct: 3426 LNTSTPITSSTVVNSSTPITSSSVLNSSTAIAS 3458 Score = 29.1 bits (62), Expect = 0.75 Identities = 23/93 (24%), Positives = 46/93 (49%) Frame = +2 Query: 245 ASPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTA 424 +S IT+ A++ +TP + S +L +T+SS +++ + + N + + Sbjct: 620 SSTPITSSTALNTSTPITS--SSVLNSSTPITSSSVLNSSTPITSSTALNTSTPITSSSV 677 Query: 425 TQTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 +S+P+ + A S+ SS ++ S TAI S Sbjct: 678 LNSSTPITSSTALNTSTPITSSSVLNSSTAITS 710 Score = 27.9 bits (59), Expect = 1.7 Identities = 22/93 (23%), Positives = 44/93 (47%) Frame = +2 Query: 245 ASPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTA 424 +S IT+ ++++TP + S +L +T+SS +++ + N + T Sbjct: 3092 SSTAITSSSIVNSSTPITS--SSVLNSSTAITSSSILNSSTPITSSSILNSSTPITSSTV 3149 Query: 425 TQTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 +S+P+ + S+ SS ++ S TAI S Sbjct: 3150 VNSSTPITSSTTLNTSTPITSSSVLNSSTAITS 3182 Score = 25.8 bits (54), Expect = 7.0 Identities = 21/93 (22%), Positives = 44/93 (47%) Frame = +2 Query: 245 ASPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTA 424 +S IT+ ++++TP + S ++ +T+SS +++ + N + T Sbjct: 3236 SSTPITSSSVLNSSTPITS--STVVNTSTPITSSSVLNSSTPITSSTVVNTSTPITSSTV 3293 Query: 425 TQTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 +S+P+ + S+ SS ++ S TAI S Sbjct: 3294 VNSSTPITSSTTLNTSTPITSSSVLNSSTAITS 3326 Score = 25.4 bits (53), Expect = 9.3 Identities = 20/93 (21%), Positives = 44/93 (47%) Frame = +2 Query: 245 ASPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTA 424 +S IT+ ++ +TP + S +L +T+S+ +++ + + N + + Sbjct: 932 SSTPITSSTGLNTSTPITS--SSVLNSSTPITSSTVLNSSTPITSSTALNTSTPITSSSV 989 Query: 425 TQTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 +S+P+ + S+ SS ++ S TAI S Sbjct: 990 LNSSTPITSSSVLNTSTPITSSSVLNSSTAITS 1022 >SPCPB1C11.01 |amt1||ammonium transporter Amt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 497 Score = 28.3 bits (60), Expect = 1.3 Identities = 15/55 (27%), Positives = 23/55 (41%) Frame = -2 Query: 585 WLVFHHGECRIGHWIPALHAREAIAVPLSIKRDDGFIDDWISAPLAASGELVCVA 421 W V + R HW A+A ++I GF+ W + + A G + C A Sbjct: 271 WCVIDFAKNR--HWSVVGFCEGAVAGLVAITPGSGFVPPWAAVVIGALGAVFCYA 323 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 27.5 bits (58), Expect = 2.3 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 7/30 (23%) Frame = +3 Query: 300 PCDPSS----W---RVVARSPLRLRWMQEG 368 P DPS W R+VA S +RLRW+++G Sbjct: 1272 PIDPSEKLGFWDKIRLVAHSQIRLRWLEDG 1301 >SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 2812 Score = 27.1 bits (57), Expect = 3.0 Identities = 12/54 (22%), Positives = 24/54 (44%) Frame = +1 Query: 106 DKVIQGRIVTALNKKWHPEHFVCNTCRKPIDGAKFHQHNNGVHCVPCFTKHHSP 267 D + G + + +K +HP+ + +TCRK I + + +H+ P Sbjct: 2536 DTIPLGEYLDSAHKVYHPKEWSLSTCRKLIAEKQMEDLETRLKVYDLVCRHYRP 2589 >SPAC56F8.13 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 101 Score = 26.6 bits (56), Expect = 4.0 Identities = 11/20 (55%), Positives = 12/20 (60%) Frame = +3 Query: 12 TQHNTKVKKYKNNHRHNHVN 71 TQ KK KN + HNHVN Sbjct: 4 TQKKKNEKKTKNPYSHNHVN 23 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 26.6 bits (56), Expect = 4.0 Identities = 19/92 (20%), Positives = 40/92 (43%) Frame = +2 Query: 248 SPNITARGAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTAT 427 S ++ + ++ S ++ S LA ++S A + S ++ S PT++ Sbjct: 116 SSSLASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSATPTSS 175 Query: 428 QTSSPLAARGAEIQSSIKPSSRLMLSGTAIAS 523 TSS L++ A ++ + L+ T A+ Sbjct: 176 ATSSSLSSTAASNSATSSSLASSSLNSTTSAT 207 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 26.2 bits (55), Expect = 5.3 Identities = 14/44 (31%), Positives = 21/44 (47%) Frame = +2 Query: 269 GAMDAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRL 400 G ++ + T+ +K CRG + S VDA A+ G N L Sbjct: 636 GQKYGSSSTSTIIAKCPMCRGNIVQDSLVDATILQAIHGPLNSL 679 >SPCC613.07 |||zf-HIT|Schizosaccharomyces pombe|chr 3|||Manual Length = 345 Score = 25.8 bits (54), Expect = 7.0 Identities = 12/44 (27%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = +1 Query: 169 VCNTCRKPIDGAKFHQHNNGVHCVPCFTKH-HSPRCHGCGDPIT 297 +C+TC+K + + ++ C+ C +H +C G DP T Sbjct: 8 ICSTCQKNASKYRCPRCDSRFCCLECNLEHKRLTKCSGERDPAT 51 >SPBC646.04 |pla1||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 566 Score = 25.4 bits (53), Expect = 9.3 Identities = 14/47 (29%), Positives = 22/47 (46%) Frame = -2 Query: 612 DELSAFTAEWLVFHHGECRIGHWIPALHAREAIAVPLSIKRDDGFID 472 +E A A +F +G R+G + P + VP + RD+ F D Sbjct: 72 NEKMANEAGGKIFTYGSYRLGVYGPGSDIDTLVVVPKHVSRDNFFQD 118 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/47 (23%), Positives = 20/47 (42%) Frame = +1 Query: 523 TCMKCRNPVTDSTFSVMENQPLCGKCA*FITNI*IKSPIEMYSNTFR 663 TC C+ T + QPLC C F+ + P+ + ++ + Sbjct: 634 TCTNCQTRTTPLWRRSPDGQPLCNACGLFMKINGVVRPLSLKTDVIK 680 >SPCC2H8.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 266 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +3 Query: 9 ITQHNTKVKKYKNNHRHNHVNKG 77 IT+ +TK KK K+N N+V G Sbjct: 110 ITKPHTKTKKLKSNSIFNYVRNG 132 >SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.4 bits (53), Expect = 9.3 Identities = 7/21 (33%), Positives = 15/21 (71%) Frame = +3 Query: 12 TQHNTKVKKYKNNHRHNHVNK 74 T N++ +++NH H+H++K Sbjct: 533 THENSQEHHHEHNHNHDHIHK 553 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 25.4 bits (53), Expect = 9.3 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 338 TTSSAVDARRSLAVEGSWNRLAVPIAPTATQTSS 439 TTS++ S+ G + L+ PI PT TS+ Sbjct: 167 TTSTSCTTSTSIPPTGGSSSLSTPITPTVPPTST 200 >SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1004 Score = 25.4 bits (53), Expect = 9.3 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 26 ESKKI*KQSPAQSCQQRLKPPPSVTA 103 E+ K+ PA+S +Q L P PS+T+ Sbjct: 904 ETPKVISARPAESVKQPLTPVPSMTS 929 >SPAC5D6.02c |mug165||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 25.4 bits (53), Expect = 9.3 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = -2 Query: 399 SLFHEPSTAKLLLASTADEVVSVPRHAKSLDHTVS 295 S FH+PS+ L AD SVP ++S + S Sbjct: 32 SYFHKPSSTGLFATLVADTNSSVPSASRSPESIAS 66 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,088,574 Number of Sequences: 5004 Number of extensions: 61978 Number of successful extensions: 217 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 379359666 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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