Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31118
(746 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 51 2e-07
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 47 2e-06
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 29 0.93
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 27 2.1
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 27 3.8
SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |... 27 3.8
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 5.0
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 26 5.0
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 26 6.6
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 50.8 bits (116), Expect = 2e-07
Identities = 24/59 (40%), Positives = 40/59 (67%)
Frame = +3
Query: 378 NESEDAKEQRRRNHAINQQLKQDRELYQATHRLLLLGAGESGKSTIVKQMRILHVNGFS 554
N ++ E ++ N I +Q++ + + +++LLLGA +SGKSTI KQ++IL+ NGFS
Sbjct: 5 NGLSESGESKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGFS 63
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 47.2 bits (107), Expect = 2e-06
Identities = 30/84 (35%), Positives = 45/84 (53%), Gaps = 1/84 (1%)
Frame = +3
Query: 471 RLLLLGAGESGKSTIVKQMRILHVNGFSXXXXXXXXXXXXXNVRDAIITITGAMSTLTPP 650
++LLLGAG+SGK+TI+KQMR+L+ GFS N+ ++ + AM
Sbjct: 76 KVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLCLLLEAMDNSNVS 135
Query: 651 IPLEKPEN-KPRVDYIQDVASQPD 719
+ PEN K R ++ SQP+
Sbjct: 136 L---LPENEKYRAVILRKHTSQPN 156
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 28.7 bits (61), Expect = 0.93
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 4/42 (9%)
Frame = +3
Query: 414 NHAINQQLKQDRELYQATHRLLLL----GAGESGKSTIVKQM 527
N+A+NQ ++DREL Q H L LL G SG I+K++
Sbjct: 493 NNAVNQLSEKDRELPQIEHILPLLRRGIGIHHSGLLPILKEV 534
>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 695
Score = 27.5 bits (58), Expect = 2.1
Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 7/63 (11%)
Frame = +3
Query: 360 SLGGHRNESEDAKEQRRRNHAINQQ--LKQDR-----ELYQATHRLLLLGAGESGKSTIV 518
S+ + S+ K Q+ N + N L QD+ +L + +LLLLG +SGK T++
Sbjct: 238 SVKSMKKVSQQLKPQKNTNDSNNDHTLLSQDQLIELSKLVKPRTKLLLLGPPKSGKKTLL 297
Query: 519 KQM 527
++
Sbjct: 298 SRL 300
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 26.6 bits (56), Expect = 3.8
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +3
Query: 423 INQQLKQDRELYQATHRLLLL 485
+N +LK DRE+YQ + L +
Sbjct: 103 VNMELKSDREMYQLVQKFLAI 123
>SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 760
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +1
Query: 25 PTFDTMYL*SLVISGRDIS 81
PTF+TM L SL+I+G D S
Sbjct: 733 PTFETMGLYSLLIAGEDTS 751
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +3
Query: 642 TPPIPLEKPENKPRVDYIQDVASQ 713
+PP P KP +KP V Y + +Q
Sbjct: 629 SPPTPRTKPSHKPPVSYKNKLVTQ 652
>SPBC354.05c |sre2||membrane-tethered transcription factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 793
Score = 26.2 bits (55), Expect = 5.0
Identities = 17/49 (34%), Positives = 29/49 (59%)
Frame = -3
Query: 219 VLLRRHISYATVVSSAHEFIANLATKYPQLKSEASSLLDTHLTITTADI 73
V L R ++ AT++S A E+I +L +K +L E + +L L+ T+ I
Sbjct: 473 VPLSRKLNKATILSKATEYIKSLQSKNKKL-IEENKILQKRLSEYTSVI 520
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 25.8 bits (54), Expect = 6.6
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +3
Query: 423 INQQLKQDRELYQATHRLLLLGAGESGKSTIVKQMRILHVNGFS 554
+ QQ ++RE+ R+LLLG +GK+TI+K + VN S
Sbjct: 7 LRQQKLKEREV-----RVLLLGLDNAGKTTILKCLLNEDVNEVS 45
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,641,949
Number of Sequences: 5004
Number of extensions: 48835
Number of successful extensions: 150
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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