BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31117 (599 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 161 5e-41 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 161 5e-41 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 101 6e-23 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 101 6e-23 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 101 6e-23 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 62 6e-11 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 38 8e-04 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 37 0.003 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 31 0.17 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.8 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 2.8 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 8.5 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 161 bits (392), Expect = 5e-41 Identities = 78/128 (60%), Positives = 91/128 (71%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXX 267 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 21 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKR 80 Query: 268 XXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHY 447 Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Y Sbjct: 81 KKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDRLY 138 Query: 448 CGKCHSTM 471 CG+CH T+ Sbjct: 139 CGRCHLTL 146 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 1 MQIFVKTLTGKTITLEVESS 20 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 161 bits (392), Expect = 5e-41 Identities = 78/128 (60%), Positives = 91/128 (71%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVXXXXXXXXXX 267 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 21 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKR 80 Query: 268 XXXNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHY 447 Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Y Sbjct: 81 KKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDRLY 138 Query: 448 CGKCHSTM 471 CG+CH T+ Sbjct: 139 CGRCHLTL 146 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 1 MQIFVKTLTGKTITLEVESS 20 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 21 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 1 MQIFVKTLTGKTITLEVESS 20 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 21 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 1 MQIFVKTLTGKTITLEVESS 20 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 21 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 97 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 146 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 173 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 222 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 249 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 298 Score = 101 bits (243), Expect = 6e-23 Identities = 47/50 (94%), Positives = 50/50 (100%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 DTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQKESTLHLV Sbjct: 325 DTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 374 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 1 MQIFVKTLTGKTITLEVESS 20 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 77 MQIFVKTLTGKTITLEVESS 96 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 153 MQIFVKTLTGKTITLEVESS 172 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 229 MQIFVKTLTGKTITLEVESS 248 Score = 39.9 bits (89), Expect = 3e-04 Identities = 19/20 (95%), Positives = 19/20 (95%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 MQIFVKTLTGKTITLEVE S Sbjct: 305 MQIFVKTLTGKTITLEVESS 324 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 62.1 bits (144), Expect = 6e-11 Identities = 24/50 (48%), Positives = 37/50 (74%) Frame = +1 Query: 88 DTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+++ S LHLV Sbjct: 21 DKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLV 70 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/20 (60%), Positives = 16/20 (80%) Frame = +3 Query: 27 MQIFVKTLTGKTITLEVEPS 86 M I VKTLTGK I L+++P+ Sbjct: 1 MLIKVKTLTGKEIELDIDPN 20 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 38.3 bits (85), Expect = 8e-04 Identities = 15/42 (35%), Positives = 29/42 (69%) Frame = +1 Query: 94 IENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 219 I +K KIQ ++ +Q+LI++G+ L D +T+ +YNI+++ Sbjct: 24 ISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 36.7 bits (81), Expect = 0.003 Identities = 18/51 (35%), Positives = 29/51 (56%) Frame = +1 Query: 85 QDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 237 + ++ +K I I +QRLI+AG+ L+D +L Y IQ ++HLV Sbjct: 23 ESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLV 73 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 30.7 bits (66), Expect = 0.17 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = -3 Query: 549 NPSYTAVASVSYRLEGTYLFVVVFEDHRAVTLPAV 445 NP+YT SY L TYL F++HR + AV Sbjct: 144 NPAYTNSHQNSYSLNETYLSYDFFDNHRGASSSAV 178 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 26.6 bits (56), Expect = 2.8 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -2 Query: 217 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 131 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 26.6 bits (56), Expect = 2.8 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = +1 Query: 331 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 489 V+R Y ++ +N + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 8.5 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +1 Query: 139 PNQQRLIFAGKQLEDGRTLSDYNI 210 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,321,764 Number of Sequences: 5004 Number of extensions: 44186 Number of successful extensions: 122 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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