Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31108
(751 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 40 0.002
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 37 0.016
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 36 0.029
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 35 0.050
At5g44590.1 68418.m05464 hypothetical protein 32 0.35
At3g21320.1 68416.m02693 hypothetical protein 30 1.9
At2g05120.1 68415.m00539 expressed protein 29 2.5
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 29 2.5
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 29 3.3
At5g18720.1 68418.m02221 hypothetical protein predicted proteins... 29 3.3
At2g42480.1 68415.m05255 meprin and TRAF homology domain-contain... 29 3.3
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 29 4.4
At2g35320.1 68415.m04331 expressed protein 28 5.8
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 39.5 bits (88), Expect = 0.002
Identities = 35/115 (30%), Positives = 52/115 (45%)
Frame = +2
Query: 188 ADATDKVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPYYS 367
++ATD ED YD+I LS+ ++VLL+E GG P + +
Sbjct: 43 SNATDFASED-YYDYIIVGGGTAGCPLAATLSQ--SFRVLLLERGGVPYNRPNV-MSHDG 98
Query: 368 NMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGSSSINLMFYVRGNKADYD 532
+ T D P + +G RG+VLGGSS+IN FY R +K ++
Sbjct: 99 FLTTLTDVNNFDSPAQSFI---SEEGVPNARGRVLGGSSAINAGFYSRADKQFFE 150
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 36.7 bits (81), Expect = 0.016
Identities = 45/156 (28%), Positives = 69/156 (44%), Gaps = 2/156 (1%)
Frame = +2
Query: 185 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 361
P TD K + ++D+I LSE + VL++E GG+P +
Sbjct: 23 PYMTTDPKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEDKK 80
Query: 362 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGNKADYDEW 538
Y G S +T+ +++ + G RG+VLGGSS+IN FY R + DE+
Sbjct: 81 Y--YGYS---LINTDEYSSVAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRAS----DEF 131
Query: 539 AADGNEGWSFEEVLPYFKKSESFMGKFDAEATKYHS 646
GW + V +K ES + F E T++ S
Sbjct: 132 VK--KAGWDKDLVQESYKWVESKV-VFMPELTRWQS 164
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 35.9 bits (79), Expect = 0.029
Identities = 31/92 (33%), Positives = 40/92 (43%)
Frame = +2
Query: 302 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 481
VL++E GG+P P ++MG S + + P + G R +VLGG
Sbjct: 97 VLVLERGGSP-----YENPTATDMGNSVNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGG 151
Query: 482 SSINLMFYVRGNKADYDEWAADGNEGWSFEEV 577
S IN FY R DY E A W EEV
Sbjct: 152 SVINGGFYSRAGN-DYVEEAE-----WEMEEV 177
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 35.1 bits (77), Expect = 0.050
Identities = 45/156 (28%), Positives = 69/156 (44%), Gaps = 2/156 (1%)
Frame = +2
Query: 185 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 361
P TD K + ++D+I LSE + VL++E GG+P + +
Sbjct: 2 PYMTTDAKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEERK 59
Query: 362 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGNKADYDEW 538
Y G S +T+ +++ + G RG+VLGGSS+IN FY R + DE+
Sbjct: 60 Y--FGYS---LLNTDEYSSVAQSFTSVDGIENYRGRVLGGSSAINGGFYSRAS----DEF 110
Query: 539 AADGNEGWSFEEVLPYFKKSESFMGKFDAEATKYHS 646
GW V +K ES + F E T++ S
Sbjct: 111 VK--KAGWDKGLVQESYKWVESKV-VFMPELTQWQS 143
>At5g44590.1 68418.m05464 hypothetical protein
Length = 349
Score = 32.3 bits (70), Expect = 0.35
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 536 WAADGNEGWSFEEVLPYFKKSESFMGKFDAEATKYHSKGGYLSVASDDNMHEIED 700
W + EGW +E+L +K+ ++G + E SKGG L + DD + +D
Sbjct: 159 WPSTELEGWEIDEIL--IEKARDYLGLSELEIPT--SKGGRLCIHVDDALSPCQD 209
>At3g21320.1 68416.m02693 hypothetical protein
Length = 540
Score = 29.9 bits (64), Expect = 1.9
Identities = 17/54 (31%), Positives = 21/54 (38%)
Frame = -1
Query: 604 AFTFLKVGQDFLETPAFVSIGSPFVVIGLVTPNVKHQIDAAASAEHLTTWPSAS 443
AF GQD LETP + SPF PN + +D W + S
Sbjct: 390 AFMVPVYGQDSLETPFRFPVSSPFSHSYFPPPNARTTVDQTNPFGQFQRWSNTS 443
>At2g05120.1 68415.m00539 expressed protein
Length = 1234
Score = 29.5 bits (63), Expect = 2.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 4/35 (11%)
Frame = +2
Query: 515 NKADYDEW----AADGNEGWSFEEVLPYFKKSESF 607
N AD D+W A NEGWS EE L + + F
Sbjct: 1122 NAADQDDWDRHHQASTNEGWSEEETLQNLRNTALF 1156
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 29.5 bits (63), Expect = 2.5
Identities = 28/100 (28%), Positives = 40/100 (40%), Gaps = 3/100 (3%)
Frame = +2
Query: 302 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 481
VLL+E G +P I + S G + + P + R G R +VLGG
Sbjct: 70 VLLLERGDSPYNNPNITR--LSAFGAALSDLSESSPSQ---RFVSEDGVINARARVLGGG 124
Query: 482 SSINLMFYVRGNKADYDEWAADG---NEGWSFEEVLPYFK 592
S++N FY R DG NE + + E F+
Sbjct: 125 SALNAGFYTRAGTKYVRNMGWDGALANESYQWVEAKVAFQ 164
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 29.1 bits (62), Expect = 3.3
Identities = 29/88 (32%), Positives = 38/88 (43%), Gaps = 6/88 (6%)
Frame = +2
Query: 302 VLLVEAGG----NPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKV 469
VL++E GG NPT AT+I N T+ +T P+ + G R +V
Sbjct: 70 VLVLERGGAPYDNPT-ATDI-----ENFATTLS---NTSPKSWSQLFISEDGVYNTRARV 120
Query: 470 LGGSSSINLMFYVRGNK--ADYDEWAAD 547
LGG S +N FY R EW D
Sbjct: 121 LGGGSVLNAGFYTRAGDEYVKETEWKTD 148
>At5g18720.1 68418.m02221 hypothetical protein predicted proteins -
Arabidopsis thaliana
Length = 552
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 321 VVILHWQPRYRNLTTVIWGHQKTGLTILNHRKGLVVPTKTRDALGHVVRCSAEAAASI*C 500
++I + +P + + W + +++ N R G TK R HV+ C+ + AS C
Sbjct: 126 LMITYLEPDSNDEKELQWFEKDLPVSVGNFRLGENKSTKDRSIFSHVIHCNERSNAS--C 183
Query: 501 FT 506
F+
Sbjct: 184 FS 185
>At2g42480.1 68415.m05255 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 743
Score = 29.1 bits (62), Expect = 3.3
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +2
Query: 524 DYDEWAADGNEGWSFEEVLPYFKKSESFMGKFD---AEATKYHSKGGYLSVASDDNMHEI 694
DYDEW D E E + + K +S K D E K + G ++ + +I
Sbjct: 255 DYDEWEQDIEERLKNLEGMEFDSKLDSLKSKLDEISLERKKSYDADGSRVQQLEERVKDI 314
Query: 695 EDLIIKAAVE 724
E LI+K+ +E
Sbjct: 315 E-LILKSKLE 323
>At5g23720.1 68418.m02780 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 929
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +3
Query: 147 WLRNVRSPAITYGQPMLPTRFSKIQTMTSSSSE 245
WL VR + TYG P R +I M +S+ E
Sbjct: 59 WLDLVRKRSATYGSSGFPHRLHRIDDMVTSAGE 91
>At2g35320.1 68415.m04331 expressed protein
Length = 307
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = -1
Query: 637 FSRLRVKFTHEAFTFLKVGQDFLETPAFVSIGSPFVVIGLVTPNVKHQ 494
F ++ +F H F F +G + E A ++ PFV I L P+ H+
Sbjct: 234 FKWIKERFNHPKFRFCAIGDGWEECAAAQALQWPFVKIDL-QPDSSHR 280
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,544,076
Number of Sequences: 28952
Number of extensions: 324631
Number of successful extensions: 869
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -