BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31104
(606 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 129 4e-31
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 124 1e-29
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 111 1e-25
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 88 8e-19
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 81 9e-17
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 64 1e-11
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 42 7e-05
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 41 2e-04
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 31 0.099
SPBC1604.03c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 2.1
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 26 3.7
SPCC622.16c |epe1||Jmjc domain chromatin associated protein Epe1... 25 6.5
SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces po... 25 8.6
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 8.6
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 129 bits (311), Expect = 4e-31
Identities = 74/184 (40%), Positives = 113/184 (61%), Gaps = 11/184 (5%)
Frame = +2
Query: 83 LNDGNTIPIVALGTGRGTAKESDSIDEVRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIA 262
L DG+ IP + LGT R ++ + AV A++ GYRHID AA+Y +E++VG GI
Sbjct: 18 LADGSKIPGLGLGTWRSEPNQT------KNAVKTALQYGYRHIDAAAIYGNEDEVGDGIK 71
Query: 263 EAIANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIATK--- 433
E+ G V R++++VT+KLW + HA + V AL+++LK L LDY+D YLIH+P++ K
Sbjct: 72 ES---G-VPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGE 127
Query: 434 ---PDDSPDNIDY-----LETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPGX 589
P D N+ Y ETW+ M+ + G R IG+SNFN T + R++ + ++P
Sbjct: 128 DKFPKDKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAV 187
Query: 590 NQIE 601
+Q+E
Sbjct: 188 HQME 191
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 124 bits (299), Expect = 1e-29
Identities = 72/184 (39%), Positives = 112/184 (60%), Gaps = 11/184 (5%)
Frame = +2
Query: 83 LNDGNTIPIVALGTGRGTAKESDSIDEVRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIA 262
L +G+ IP + LGT R DE + AV A++AGYRHIDTA +Y +E+++G+GI
Sbjct: 17 LPNGDKIPSIGLGTWRSGK------DETKNAVCAALKAGYRHIDTAHIYGNEKEIGEGIR 70
Query: 263 EAIANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIA--TKP 436
E+ G V R +++VT+KLW + H V AL+++L+ L L+YID YLIH+P A + P
Sbjct: 71 ES---G-VPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGP 126
Query: 437 DDSPDN---------IDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPGX 589
++ P N + ETWQ M++ + G R IG+SNFN + R++ + ++P
Sbjct: 127 EELPRNEKGELIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTI 186
Query: 590 NQIE 601
+Q+E
Sbjct: 187 HQME 190
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 111 bits (266), Expect = 1e-25
Identities = 71/172 (41%), Positives = 94/172 (54%), Gaps = 2/172 (1%)
Frame = +2
Query: 92 GNTIPIVALGTGRGT-AKESDSIDE-VRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAE 265
G IP+ A G G KE I+ + +V A+ AG+ HID A VY +EE+VG + E
Sbjct: 7 GPKIPVPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVALKE 66
Query: 266 AIANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIATKPDDS 445
A V R +LF+T+K+ H D + AL ESL+KLG DY+DLYL+H PI
Sbjct: 67 AN----VPRSKLFITSKVM---HNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYEKKI 119
Query: 446 PDNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPGXNQIE 601
P + E W+ M+ A GL S+GVSNF + L+ S I P NQIE
Sbjct: 120 PIS----EGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIE 167
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 88.2 bits (209), Expect = 8e-19
Identities = 52/144 (36%), Positives = 80/144 (55%), Gaps = 1/144 (0%)
Frame = +2
Query: 173 AVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIANGLVTREELFVTTKLWNDKHARDQVV 352
+VY A++ GYRHID+A +Y +E G+ I + + RE+++ T+KL ND +
Sbjct: 32 SVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEETGTKREDIWFTSKL-NDLSGYKSTL 90
Query: 353 PALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSN 532
++ S+K GL YIDL+L+H P D +E+W+ ++ + G R+IGVSN
Sbjct: 91 SSIDASVKACGLGYIDLFLLHSPYG----------DRIESWKALEKGVEEGKLRAIGVSN 140
Query: 533 FNATQITRLV-SNSYIRPGXNQIE 601
F I L+ S+ I P NQIE
Sbjct: 141 FGPHHIQELLDSHPKIIPCVNQIE 164
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 81.4 bits (192), Expect = 9e-17
Identities = 61/180 (33%), Positives = 90/180 (50%), Gaps = 1/180 (0%)
Frame = +2
Query: 65 QAPCIQLNDGNTIPIVALGTGRGTAKESDSIDEVRQAVYWAIEAGYRHIDTAAVYQDEEQ 244
++ + L +G IP +G G K ++ V QA +++GYRHIDTAAVY +E+
Sbjct: 7 ESTTVTLTNGMVIP--RIGFGAFMLKYNECYGLVTQA----LDSGYRHIDTAAVYGNEDI 60
Query: 245 VGQGIAEAIANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPI 424
G+ I + V R ++F+T+KL N A++ SL LG YIDL+LI P
Sbjct: 61 CGKAIVDWCEKNNVKRTDIFLTSKLANCSDYY-STRAAIRSSLHHLG-TYIDLFLIQSPA 118
Query: 425 ATKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRL-VSNSYIRPGXNQIE 601
K + +W+ M++ G RS+GVSN+ + L SN P NQIE
Sbjct: 119 GGKK-------SRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIE 171
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 64.5 bits (150), Expect = 1e-11
Identities = 40/145 (27%), Positives = 75/145 (51%), Gaps = 1/145 (0%)
Frame = +2
Query: 170 QAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIANGL-VTREELFVTTKLWNDKHARDQ 346
Q V A+ G R ID+A Y++E++ Q I + + + RE++ + TK+ + ++
Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFER 87
Query: 347 VVPALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSIGV 526
A+++SL++ G +D+ LIH P P + +E+W+ + ++ G IGV
Sbjct: 88 TWKAVEQSLRRTGRPKLDVVLIHSP------KWP--VRRIESWRALLQHQKEGRINKIGV 139
Query: 527 SNFNATQITRLVSNSYIRPGXNQIE 601
SN+N + ++S P NQ+E
Sbjct: 140 SNYNIHHLEEIISLGLPLPAINQVE 164
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 41.9 bits (94), Expect = 7e-05
Identities = 33/123 (26%), Positives = 56/123 (45%), Gaps = 8/123 (6%)
Frame = +2
Query: 92 GNTIPIVALGTGRGTAKESDSIDEVRQAVYWAIEAGYRHIDTAAVYQD---EEQVGQGIA 262
G + +LG E ++ + + A + G DTA +Y + E +G+ I
Sbjct: 22 GLKVSAFSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGNSETVMGKAIK 81
Query: 263 EA---IANGLVTREELF-VTTKLWNDKH-ARDQVVPALQESLKKLGLDYIDLYLIHFPIA 427
E + ++T + F TKL N +R ++ L SLK+LGL Y+D+ + H P
Sbjct: 82 ELGWDRSEYVITTKVFFGAGTKLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAHRPDP 141
Query: 428 TKP 436
+ P
Sbjct: 142 SVP 144
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 40.7 bits (91), Expect = 2e-04
Identities = 36/121 (29%), Positives = 57/121 (47%), Gaps = 14/121 (11%)
Frame = +2
Query: 95 NTIPIVALGTGRGTAKESDSIDEVRQAVYW-AIEAGYRHIDTAAVY---QDEEQVGQGIA 262
+T+P + G A S +E QAV A + G D++ +Y +EE +G+
Sbjct: 11 DTVPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGANEECIGRWFK 70
Query: 263 EAIANGLVTREELFVTTKLWNDKHAR----------DQVVPALQESLKKLGLDYIDLYLI 412
+ R+E+F+ TK +K+ D + AL SLK+LG+D IDLY +
Sbjct: 71 QT-----GRRKEIFLATKFGYEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYV 125
Query: 413 H 415
H
Sbjct: 126 H 126
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 31.5 bits (68), Expect = 0.099
Identities = 13/46 (28%), Positives = 27/46 (58%)
Frame = +2
Query: 461 YLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPGXNQI 598
+++TW+ +++ G ++GVS F ++ RL+S+ + P QI
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQI 203
>SPBC1604.03c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 330
Score = 27.1 bits (57), Expect = 2.1
Identities = 13/49 (26%), Positives = 25/49 (51%)
Frame = +2
Query: 71 PCIQLNDGNTIPIVALGTGRGTAKESDSIDEVRQAVYWAIEAGYRHIDT 217
P + N N +P++ LG G K+ D +D +A+ + ++ +DT
Sbjct: 32 PVDEKNRINAVPLLLLGCLYGKYKDDDFVDFFDKAIPLLVPQTHKGLDT 80
>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 3.7
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 419 PIATKPDDSPDNIDYLETWQGMQDARQL 502
P+AT D + ++D L T QG+ D R L
Sbjct: 428 PMATHVDQTEHDLDILVTEQGLADLRGL 455
>SPCC622.16c |epe1||Jmjc domain chromatin associated protein
Epe1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 948
Score = 25.4 bits (53), Expect = 6.5
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1
Query: 180 YTAWRTSSIESDSLAVPRPV 121
YTAW TSS +SD +P V
Sbjct: 329 YTAWLTSSNDSDKKFLPNMV 348
>SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 418
Score = 25.0 bits (52), Expect = 8.6
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +2
Query: 179 YWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIANGLVTREELFVTTK 316
Y A+EAG HIDT+ + E + +A VT E ++T K
Sbjct: 237 YCALEAGATHIDTSILGIGERNGITPLGALLARMYVTDRE-YITHK 281
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 25.0 bits (52), Expect = 8.6
Identities = 18/64 (28%), Positives = 29/64 (45%)
Frame = -1
Query: 240 SSSWYTAAVSICLYPASIAQYTAWRTSSIESDSLAVPRPVPKATMGIVLPSFSCMQGACT 61
SSS T++V+ + P S + ++W +SS E D +T I S S +
Sbjct: 933 SSSAITSSVAASVTPTSSSSASSWSSSS-EVDPSTAASATGSSTSSIATASVSGSSTSSV 991
Query: 60 AIAA 49
A A+
Sbjct: 992 ATAS 995
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,474,526
Number of Sequences: 5004
Number of extensions: 48323
Number of successful extensions: 154
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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