BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31046 (747 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.2 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.1 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.8 SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces... 26 5.0 SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizos... 26 5.0 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 26 5.0 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 6.6 SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 26 6.6 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 6.6 SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 8.7 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 8.7 SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.7 SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 8.7 SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 8.7 >SPAC1006.07 |||translation initiation factor eIF4A|Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 28.3 bits (60), Expect = 1.2 Identities = 9/21 (42%), Positives = 17/21 (80%) Frame = +1 Query: 223 AIDLLTNDECRLLLEVEDFFN 285 +I+ +TND+ R++ E+E F+N Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 27.5 bits (58), Expect = 2.1 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 356 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 445 STR + N+VP N L I+C + NK Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.6 bits (56), Expect = 3.8 Identities = 21/89 (23%), Positives = 37/89 (41%), Gaps = 2/89 (2%) Frame = +1 Query: 487 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSP- 663 P+ S V P P A++ AS+P + S P ++ + T + P Q P Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952 Query: 664 -PGPATQLLLSYAQQAQCTALAPSASLAP 747 P P T + + A + ++ S ++ P Sbjct: 953 HPNPYTP-VAPQSPVAAASRISSSPNMPP 980 >SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 711 Score = 26.2 bits (55), Expect = 5.0 Identities = 17/62 (27%), Positives = 26/62 (41%) Frame = +1 Query: 562 SPFVTSQPTEELLREFETVYGAVELTHLTPPQSPPGPATQLLLSYAQQAQCTALAPSASL 741 +PF PT +R +++YG+ P SPP A Q + Y + + A S Sbjct: 210 TPFSIHSPTASSVRSCQSIYGS--------PLSPPNSAFQAEMPYFPISPAISCASSCPS 261 Query: 742 AP 747 P Sbjct: 262 TP 263 >SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 297 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 618 LWCCRVNAFNTAAESAGTRDSVAS 689 LW CR N+F+ A +DSV+S Sbjct: 124 LWDCRSNSFSPIQVLADAKDSVSS 147 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 26.2 bits (55), Expect = 5.0 Identities = 12/37 (32%), Positives = 18/37 (48%) Frame = +1 Query: 634 LTHLTPPQSPPGPATQLLLSYAQQAQCTALAPSASLA 744 +TH TPP PP P L +++ +A S+A Sbjct: 902 ITHPTPPPPPPLPVKTSLNTFSHPDSVNIVANDTSVA 938 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Frame = +1 Query: 466 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 618 +E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+ Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/47 (27%), Positives = 22/47 (46%) Frame = +3 Query: 93 QDKLVHLHKTPTSLDINPSGLLFAFVELNHYNNECESEGVMGCGHRF 233 +D H K I+P LLF F E NH +++ ++ G ++ Sbjct: 345 EDSCFHKIKAAQWHQISPRSLLFQFQEQNHIHHKKIRRKLLAAGWKW 391 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Frame = -3 Query: 640 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 506 A T H P + P+ +QW +S +DL+ ++ V+ +E K Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791 >SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 731 Score = 25.4 bits (53), Expect = 8.7 Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +1 Query: 424 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 540 +D CKQ+ +FS E++ D L SI +S + ++ P Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 25.4 bits (53), Expect = 8.7 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +1 Query: 241 NDECRLLLEVEDFFNDDC 294 NDECR L + FF+ +C Sbjct: 535 NDECRRLKQCNHFFHREC 552 >SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 95 Score = 25.4 bits (53), Expect = 8.7 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = +3 Query: 96 DKLVHLHKTPTSLDINPSGLLFAFVELNH 182 D + H K +S NPS LLF L H Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87 >SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2310 Score = 25.4 bits (53), Expect = 8.7 Identities = 14/49 (28%), Positives = 23/49 (46%) Frame = -1 Query: 366 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 220 W T+ V R S RKV + + + EE +++ ALI + + R Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191 >SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual Length = 304 Score = 25.4 bits (53), Expect = 8.7 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Frame = +3 Query: 72 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 206 L + ++KLV+L T T LD + ++ A V+ N Y N C+++ Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,780,034 Number of Sequences: 5004 Number of extensions: 53774 Number of successful extensions: 154 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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