Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31039
(758 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 163 3e-41
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 134 1e-32
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 54 3e-08
SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c... 31 0.13
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 30 0.31
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 29 0.54
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 29 0.72
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 28 1.3
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 27 2.2
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 27 2.9
SPBC651.09c |||RNA polymerase II associated Paf1 complex |Schizo... 27 3.8
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 27 3.8
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 5.1
SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex s... 26 5.1
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 26 6.7
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 25 8.9
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 163 bits (395), Expect = 3e-41
Identities = 83/166 (50%), Positives = 114/166 (68%), Gaps = 3/166 (1%)
Frame = +2
Query: 194 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNSI 364
G+ TLIPGDG+G E AV E+FK A++P++FE + + N + L + + S+
Sbjct: 19 GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78
Query: 365 AVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDVDCIII 544
NK+ +KGIL TP F G + N+ LR LD+YA++V +K++P K RH +VD II
Sbjct: 79 KRNKVGLKGILFTP-FEKGGHT-SFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAII 136
Query: 545 REQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVEDG 682
RE TEGEYS LEH+SVPGVVE LKIIT KS+RIA+FAFD+A+++G
Sbjct: 137 RENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNG 182
Score = 28.7 bits (61), Expect = 0.95
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +3
Query: 681 GRKKVTAVHKANIME 725
GRK VT +HKANIM+
Sbjct: 182 GRKSVTCIHKANIMK 196
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 134 bits (324), Expect = 1e-32
Identities = 68/165 (41%), Positives = 104/165 (63%), Gaps = 2/165 (1%)
Frame = +2
Query: 194 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPTLSAPLEDVVNSIA 367
G T+I GDG+GPE+ +V+ +FKAA +P+++E + N T + P +D S+
Sbjct: 44 GNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTTIP-DDAKESVR 102
Query: 368 VNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDVDCIIIR 547
NK+ +KG LATP G + ++N+ LR L+ANV S+ K + +V+ ++IR
Sbjct: 103 KNKVALKGPLATP--IGKGHV-SMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLIR 159
Query: 548 EQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVEDG 682
E TEGEYS +EHE +PGVV+ +K+IT A SER+ ++AF YA + G
Sbjct: 160 ENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTG 204
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 53.6 bits (123), Expect = 3e-08
Identities = 53/171 (30%), Positives = 81/171 (47%), Gaps = 13/171 (7%)
Frame = +2
Query: 197 RIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFE-SFFFSEVNP-------TLSAPLEDV 352
RI LIP DG+G E+V A + + + ++P + F F +++ T A E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME--NLPAKHKLKFDFIDLDAGWGTFERTGKALPERT 63
Query: 353 VNSIAVN-KICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDV 529
V + + G + +P G + + LR + LYANV VKSL K + V
Sbjct: 64 VERLKTECNAALFGAVQSPTHKVAGYSSPI-VALRKKMGLYANVRPVKSLDGAK--GKPV 120
Query: 530 DCIIIREQTEGEYSALEH--ESVPG--VVECLKIITAAKSERIAKFAFDYA 670
D +I+RE TE Y E ++ PG V E ++ I+ S +I K AF+ A
Sbjct: 121 DLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIA 171
>SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 680
Score = 31.5 bits (68), Expect = 0.13
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 89 RNIFRAVMQGSQHVGKGVHTSSVTTEKNQPRATKEGRIKCTLIPGD 226
RN F + ++ G G T S TT KNQPR+ ++ R+ + G+
Sbjct: 196 RNNFSGLESSTK--GNGTFTVSKTTRKNQPRSRRDPRLSWKALGGE 239
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 30.3 bits (65), Expect = 0.31
Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 8/101 (7%)
Frame = +2
Query: 203 KCTLIPGDGVGPELVYAVQEVFKAA-----SIPVDFESFFFSEVN-PTLSAPLED-VVNS 361
K ++PGD +GPE+V + EV K + ++FE + PL D V +
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 362 IAVNKICIKGILATPDFSHTG-ELQTLNMKLRNALDLYANV 481
+ G + P++++ + +KLR ++ ++AN+
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANL 105
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 29.5 bits (63), Expect = 0.54
Identities = 21/78 (26%), Positives = 41/78 (52%), Gaps = 2/78 (2%)
Frame = +3
Query: 495 HCPT*SAGTRTWTASSSESRP--RESTQLWNMNPFPAWWSV*RSSPQRNPSVSRNSLSTT 668
H P +A + + ++S+S P + ++ NP P W ++PQ P++S N S
Sbjct: 405 HPPPWAAASTSVSSSTSSPAPWAKPASSAAPSNPAP--WQQ-PAAPQSAPALSMNPSSLP 461
Query: 669 PWKMGRKKVTAVHKANIM 722
PW+ ++ +AV +N++
Sbjct: 462 PWQQPTQQ-SAVQPSNLV 478
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 29.1 bits (62), Expect = 0.72
Identities = 25/83 (30%), Positives = 39/83 (46%)
Frame = +2
Query: 236 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 415
P A+Q++ S P+D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 416 HTGELQTLNMKLRNALDLYANVV 484
+ LN+ + A L VV
Sbjct: 592 DENQEDHLNLTVHKADFLRMRVV 614
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +2
Query: 284 PVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNAL 463
P F S N L D + ++ +KI I +LAT + + ++ T +L
Sbjct: 245 PASFTSSRNKRSNERLFGMTSDSLQTVTPDKIAILDLLATQSIASSTDMLTAFRELTQDF 304
Query: 464 DLYANVVHVK 493
+YA+ + ++
Sbjct: 305 PIYAHYLSIQ 314
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.5 bits (58), Expect = 2.2
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -2
Query: 379 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVHW 284
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 27.1 bits (57), Expect = 2.9
Identities = 27/106 (25%), Positives = 44/106 (41%), Gaps = 10/106 (9%)
Frame = +2
Query: 104 AVMQGSQHVGKGVHTSSVTTEK-NQPRATKEGRIKCTLIPGDGVGPELVYAVQEVFKAAS 280
+V +GS +G+ + T + PR+ G I DG GP L + E K
Sbjct: 381 SVSEGSGVLGRQANWDDFTNGRFGDPRSPAYGEI-------DGYGPTLHFPPSEALKLWG 433
Query: 281 IPVD-------FESFFFSEVN--PTLSAPLEDVVNSIAVNKICIKG 391
PVD F+ S+++ P + P+E +IA + + G
Sbjct: 434 YPVDESDITSLFQKHIMSDISAIPWIDEPVEVETKTIAKYLLKLNG 479
>SPBC651.09c |||RNA polymerase II associated Paf1 complex
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 560
Score = 26.6 bits (56), Expect = 3.8
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +3
Query: 618 SSPQRNPSVSRNSLSTTPWKMGRKKVTAVHKANIMEAGRRTVPTQ 752
+SPQ +PS+S + +ST V A KA+ + + +P++
Sbjct: 469 TSPQPSPSLSTSIMSTPKLNPSESVVVASEKASSPDLSPKLLPSE 513
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -2
Query: 382 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVHWNAG 275
T L++ + ++++F CTK W +H +K+ K++ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 5.1
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +2
Query: 311 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLY 472
S++N A LED N +C++ +A S LQ +K R+ L Y
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQ--GVKFRSKLMAY 592
>SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex
subunit Tim8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 98
Score = 26.2 bits (55), Expect = 5.1
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +1
Query: 121 STCWKGCTHKFGNNGEKSTE 180
STCW C GN +KS E
Sbjct: 42 STCWPKCIGNIGNKLDKSEE 61
>SPAC22E12.11c |set3||histone lysine methyltransferase
Set3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +3
Query: 618 SSPQRNPSVSRNSLS-TTPWKMGRKKVTAVHK 710
+SP ++P S + L T+P K G +T VHK
Sbjct: 574 ASPLQHPRTSPDMLDKTSPCKRGLGTITTVHK 605
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -2
Query: 241 LGSYTIPRNQGAFDTTFLCCPRLIFLRCYRTCVYTL 134
L +YT PR + A T R+++L+ + +YT+
Sbjct: 913 LNAYTKPRYKSAASTRLNIYQRILYLKVFTWPLYTI 948
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,174,575
Number of Sequences: 5004
Number of extensions: 68093
Number of successful extensions: 227
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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