BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV31032 (603 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 31 0.13 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 28 0.91 SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 28 0.91 SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 26 4.9 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 6.4 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 8.5 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 31.1 bits (67), Expect = 0.13 Identities = 31/82 (37%), Positives = 40/82 (48%), Gaps = 8/82 (9%) Frame = -3 Query: 547 YLGM*APSATKPFSSAV*STL*VT-----PSGPTYS*NP--LTLMPSFSVPTFSTLPASS 389 Y G+ +P T PFSSA+ +T PS P YS +P + PS+S + S P S Sbjct: 1531 YKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSP 1590 Query: 388 ARILFSVSYK*W*PS-GATSLS 326 + S SY PS ATS S Sbjct: 1591 SYSPTSPSYSPTSPSYSATSPS 1612 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 28.3 bits (60), Expect = 0.91 Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 1/66 (1%) Frame = -3 Query: 526 SATKPFSSAV*STL*VTPSGPTYS*NPLTL-MPSFSVPTFSTLPASSARILFSVSYK*W* 350 ++T SS+ S++ +P+G S TL M S P FS + S+++ SVSY Sbjct: 130 TSTVDISSSTSSSVINSPTGTAVSSQISTLSMSPSSTPVFSPSASVSSKVASSVSYVSSE 189 Query: 349 PSGATS 332 PS ++S Sbjct: 190 PSDSSS 195 >SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 198 Score = 28.3 bits (60), Expect = 0.91 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = +3 Query: 327 DNDVAPEGYHYLYETENKI 383 DND+ PE Y LYE E+K+ Sbjct: 132 DNDLEPEVYDILYEEESKL 150 >SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 25.8 bits (54), Expect = 4.9 Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 4/49 (8%) Frame = +1 Query: 346 KATTTCTRPRTRFSLKKPA----RSRTLAPKTKASRSRDSTNTLAPTVS 480 K TTT + T FS KPA ++ AP + ++ ST PT S Sbjct: 286 KGTTTTSSAGTGFSFGKPATTEDTNKPTAPNSAFTKPATSTGDNKPTFS 334 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 25.4 bits (53), Expect = 6.4 Identities = 17/65 (26%), Positives = 23/65 (35%), Gaps = 3/65 (4%) Frame = +1 Query: 82 DPVLLEVPEEPTSEPRRTSANTLVMLTRDPAXXXXXXXXXXXXXQSHPHTLPARWS---H 252 D +L + P P PR + + +LTRDP +HP W H Sbjct: 893 DAILSDEPLYPIHMPRDSVSILQQLLTRDPKKRLGSGPNDAEDVMTHPFFSNINWDDIYH 952 Query: 253 PHTLP 267 T P Sbjct: 953 KRTQP 957 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 25.0 bits (52), Expect = 8.5 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +1 Query: 358 TCTRPRTRFSLKKPARSRTLAPKTKASRSRDSTNT 462 T RP + +LK P S + AP++ +S + S T Sbjct: 500 TVCRPHKKVTLKMPLNSGSSAPQSPSSNTSASVLT 534 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,702,603 Number of Sequences: 5004 Number of extensions: 28769 Number of successful extensions: 95 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 93 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 264253462 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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