Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV31021
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51010.1 68416.m05585 expressed protein 33 0.23
At3g23930.1 68416.m03006 expressed protein 32 0.41
At5g45520.1 68418.m05591 hypothetical protein 30 1.7
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 30 1.7
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 30 1.7
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 2.2
At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 29 2.9
At5g03660.1 68418.m00325 expressed protein low similarity to out... 29 2.9
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 29 2.9
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 3.8
At5g25070.1 68418.m02971 expressed protein 29 3.8
At1g01670.1 68414.m00085 U-box domain-containing protein 29 3.8
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 6.7
At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to ... 28 6.7
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 6.7
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 28 6.7
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 8.8
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 27 8.8
>At3g51010.1 68416.m05585 expressed protein
Length = 188
Score = 32.7 bits (71), Expect = 0.23
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 621
I ++R + +KKRQA++Q K + +++K A ER +QLEEE
Sbjct: 125 IGQRRAFILSEKKKRQALVQEAKRKKRIKQ---VERKMAAVARDRAWAERLIELQQLEEE 181
Query: 622 KKISLS 639
KK S+S
Sbjct: 182 KKKSMS 187
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 31.9 bits (69), Expect = 0.41
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 184 KQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 291
K + G IK +D++ + QLKE EWRK+R
Sbjct: 21 KDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENF-----GLSNAQLERNKTK 603
++E+ ++ E E++ + +++ KT P+ +K +N G SN + + +K K
Sbjct: 624 NLEDGKKHDEGKEERSLKSDEVVEEEKKTSPSEEATEKFQNKPGDQKGKSNVEGDGDKGK 683
Query: 604 EQLEEEKK 627
LEEEKK
Sbjct: 684 ADLEEEKK 691
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 29.9 bits (64), Expect = 1.7
Identities = 17/69 (24%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQLEE 618
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+ +
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEKEVK 957
Query: 619 EKKISLSIR 645
EK I+
Sbjct: 958 EKVTEKEIK 966
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 29.9 bits (64), Expect = 1.7
Identities = 26/74 (35%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK---EQLE 615
+EKR+R+EE E+K +KD N ++ + E SN +LE N K + +
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLN--LKTELEEARNSNVELELNNRKLSQDLVS 169
Query: 616 EEKKI-SLSIRIKP 654
E KI SLS KP
Sbjct: 170 AEAKISSLSSNDKP 183
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 181 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 279
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At5g05210.1 68418.m00555 nucleolar matrix protein-related contains
Pfam domain, PF04935: Surfeit locus protein 6
Length = 386
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/59 (20%), Positives = 33/59 (55%)
Frame = +1
Query: 454 RQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKI 630
+Q + + +K+++ + K+ + F ++K+ + G ++E+NK ++ + EKK+
Sbjct: 310 KQSIHKDKKRQEKNAEKWKERIEGQQKFKVEKQQKRSGNIADRIEQNKMRKIAKREKKL 368
>At5g03660.1 68418.m00325 expressed protein low similarity to outer
surface protein F [Borrelia burgdorferi] GI:466482;
contains Pfam profile PF04949: Family of unknown
function (DUF662)
Length = 173
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +1
Query: 505 MKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIRIK 651
+KD +F+ Q K E+ LS L K KE+ E+KK+ + R++
Sbjct: 18 LKDQLSESMSFSSQMKKEDDELSMKALSAFKAKEEEIEKKKMEIRERVQ 66
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 29.1 bits (62), Expect = 2.9
Identities = 23/71 (32%), Positives = 34/71 (47%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 618
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
Query: 619 EKKISLSIRIK 651
+ + + K
Sbjct: 508 LSRRYVELEAK 518
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 439 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 588
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At5g25070.1 68418.m02971 expressed protein
Length = 736
Score = 28.7 bits (61), Expect = 3.8
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 448 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE-EK 624
EKR EAEKK A + K+A + + KS N Q+E K +LE+ E
Sbjct: 565 EKRMPELEAEKKVAASTRNFKEAGR----IAAEAKSLNLEKDKTQMETGKANAELEKAEH 620
Query: 625 KISLSIR 645
+I +I+
Sbjct: 621 EIEETIK 627
>At1g01670.1 68414.m00085 U-box domain-containing protein
Length = 365
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 6/73 (8%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQ-----AMLQAMKDASKTGPNFT-IQKKSENFGLSNAQLERNKTKE 606
EE+R+RLE E KR+ M + ++A + T I E + E N+ K
Sbjct: 177 EEQRRRLEIEELKREKEQRDKMRRVREEALSSSSGVTKILYNEEVMRRREVEAELNRAKA 236
Query: 607 QLEEEKKISLSIR 645
++E+ K++ + ++
Sbjct: 237 EIEDMKRVQIELK 249
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 27.9 bits (59), Expect = 6.7
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = +1
Query: 466 EEAEKKRQAMLQAMKDASKTGPNFT--IQKKSENFGLSNAQLERNKTKEQLEEEKKISLS 639
EEA+K R+ L+ KDAS + + KK+E + +KE E+ ++ +
Sbjct: 710 EEAKKTREQALRKRKDASFKHVIISEKVDKKAEKLQTKSLPYPYT-SKEVFEQSMRMPIG 768
Query: 640 IRIKPLTIEGL 672
P TI G+
Sbjct: 769 PEFNPTTIVGV 779
>At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 304
Score = 27.9 bits (59), Expect = 6.7
Identities = 23/81 (28%), Positives = 39/81 (48%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 621
I+++ +L++ KK QA + K +K I+K E + R K +LEE
Sbjct: 44 IDKQYDKLDKLLKKLQASHEESKSVTKAPAMKAIKKTMEKDVDEVGSIARF-IKGKLEEL 102
Query: 622 KKISLSIRIKPLTIEGLSVDK 684
+ +L+ R KP +G VD+
Sbjct: 103 DRENLANRQKPGCAKGSGVDR 123
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 212 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 334
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 27.9 bits (59), Expect = 6.7
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKT---KEQL 612
+E+K + LEE +K + A+ A + G +IQ + +S + N++ +L
Sbjct: 155 LEQKLKSLEELSEKVSELKSALIVAEEEGKKSSIQMQEYQEKVSKLESSLNQSSARNSEL 214
Query: 613 EEEKKISL 636
EE+ +I+L
Sbjct: 215 EEDLRIAL 222
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +1
Query: 448 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQL---E 615
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 616 EEKK 627
EE+K
Sbjct: 504 EERK 507
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/61 (18%), Positives = 32/61 (52%)
Frame = +1
Query: 445 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 624
EE+++ EEA+++ + + ++A + ++K E + + K +E++E ++
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKR 549
Query: 625 K 627
+
Sbjct: 550 R 550
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 27.5 bits (58), Expect = 8.8
Identities = 21/80 (26%), Positives = 36/80 (45%)
Frame = +1
Query: 442 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 621
+ E ++L + + ML+ + + S N QK+ E +N E KE++E
Sbjct: 684 LHELSEKLSFKTSQMERMLENLDEKSNEIDN---QKRHEEDVTANLNQEIKILKEEIENL 740
Query: 622 KKISLSIRIKPLTIEGLSVD 681
KK S+ ++ E L VD
Sbjct: 741 KKNQDSLMLQAEQAENLRVD 760
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.130 0.360
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,395,479
Number of Sequences: 28952
Number of extensions: 159636
Number of successful extensions: 656
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 656
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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