Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30998
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 99 6e-22
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 73 3e-14
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 41 2e-04
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 36 0.006
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 30 0.31
SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy... 28 1.3
SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 28 1.3
SPCC1682.08c |||RNA-binding protein Mcp2|Schizosaccharomyces pom... 27 2.2
SPBC651.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.0
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 6.7
SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 25 8.8
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 99.1 bits (236), Expect = 6e-22
Identities = 54/185 (29%), Positives = 84/185 (45%), Gaps = 3/185 (1%)
Frame = +2
Query: 113 KPAL-PGSRVPLCASCNGNIR-GPFITALGRIWCPEHFICVNATCRRPLQDIGFVEENGQ 286
KP L G+ C SC G++R G I+A G+ P+ F C TC + L+ +GF G+
Sbjct: 246 KPVLYRGNSEKSCHSCGGSLRAGRIISASGKKLHPQCFKC--DTCSQNLEHVGFYYREGK 303
Query: 287 LYCEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFG-NNPFFLEDGL 463
YC + + +P C C I+ ++ FH C C ++F N P D L
Sbjct: 304 FYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDL 363
Query: 464 PYCEADWNELFTTKCFACGFPVEAGDRWVEALNNNYHSQCFNCTVCKKNLEGQSFFAKGG 643
+C+ ++ + KC C P+ V+ + YHSQC+ C C L + +F
Sbjct: 364 YWCQTCYDNKYAVKCKKCRKPILGIS--VKGSDGEYHSQCWTCGACNALLGDEGYFMIEN 421
Query: 644 RPFCK 658
P C+
Sbjct: 422 TPICR 426
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 73.3 bits (172), Expect = 3e-14
Identities = 45/153 (29%), Positives = 71/153 (46%), Gaps = 6/153 (3%)
Frame = +2
Query: 143 LCASCNGNIRGPFITALGRIWCPEHFIC--VNATCRRPLQDIGFVEENGQL-YCEFCFEQ 313
+CASC I G ++ ALG I+ E F C N+ I N Q+ CE + +
Sbjct: 115 ICASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFR 174
Query: 314 YIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLE-DGLPYCEADWNE 490
+ C C ++G + A+ K FH E F C C +FG N + E +G YC ++
Sbjct: 175 RLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYST 234
Query: 491 LFTTKCFACGFPV--EAGDRWVEALNNNYHSQC 583
LF +C C P+ + + + ++ N+H C
Sbjct: 235 LFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267
Score = 64.5 bits (150), Expect = 2e-11
Identities = 33/118 (27%), Positives = 53/118 (44%), Gaps = 6/118 (5%)
Frame = +2
Query: 329 CDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDG-----LPYCEADWNEL 493
C C I G + A+G +H ECF C C L + F ++D +P CE D+
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175
Query: 494 FTTKCFACGFPVEAGDRWVEALNNNYHSQCFNCTVCKKNL-EGQSFFAKGGRPFCKTH 664
C +CG + ++ ALN +H + F C++C S++ G+ +C H
Sbjct: 176 LDLLCASCGMALRG--YYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYH 231
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/60 (25%), Positives = 26/60 (43%), Gaps = 4/60 (6%)
Frame = +2
Query: 251 LQDIGFVEENGQLYCEFCFEQYIAPA----CDKCHAKIKGDCLNAIGKHFHPECFNCVYC 418
+++ FV++ G F +A A C CH+ ++ DC+ H C +C C
Sbjct: 455 VEEARFVDQEGYDSSSFDMPLNLANASSDLCYVCHSALEEDCVLLGEIRCHIGCLSCTKC 514
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 41.1 bits (92), Expect = 2e-04
Identities = 26/96 (27%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
Frame = +2
Query: 377 GKHFHPECFNCVYCGKLFGNNPFFL---EDGLPYCEADWNELFTTKCFACGFPVEAGDRW 547
G +H +CF C C K ++ L DG P C + C AC ++ D
Sbjct: 37 GHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS-----CAHTCTACRMRIK--DYA 89
Query: 548 VEALNNNYHSQCFNCTVCKKNLEGQSFFAKGGRPFC 655
+ + ++YH +CF C C+K + +F FC
Sbjct: 90 LMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFC 125
Score = 37.5 bits (83), Expect = 0.002
Identities = 19/60 (31%), Positives = 26/60 (43%), Gaps = 3/60 (5%)
Frame = +2
Query: 497 TTKCFACGFPVEAGDRWVEALNNNYHSQCFNCTVCKKNLEGQSFF---AKGGRPFCKTHA 667
+T CF CG + + + + +H CF CT C K LE GRP C + A
Sbjct: 16 STVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSCA 75
Score = 36.7 bits (81), Expect = 0.004
Identities = 33/128 (25%), Positives = 49/128 (38%), Gaps = 3/128 (2%)
Frame = +2
Query: 98 RGILNKPALPGSRVPLCASCNGNI--RGPFITALGRIWCPEHFICVNATCRRPLQDIGFV 271
+ I P+ GS V C C R I+ G +W + F C D V
Sbjct: 5 KSISKSPSSKGSTV--CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLV 62
Query: 272 E-ENGQLYCEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFF 448
+ +G+ C C A C C +IK L + +H ECF C C K ++ F
Sbjct: 63 QTSDGRPVCSSC-----AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFK 117
Query: 449 LEDGLPYC 472
++ +C
Sbjct: 118 RDNRTIFC 125
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 35.9 bits (79), Expect = 0.006
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +2
Query: 215 HFICVNATCRRPLQDIGFVEENGQLYCEFCFEQYI--APACDKCHAKIKGDCLNAIGK 382
H IC+ CR ++ GF+ G LYC FC E ++ + +C C K+ + IG+
Sbjct: 1089 HQICI--ICRDIIKQ-GFITTCGHLYCSFCLEAWLKHSSSCPMCKTKLNKNNAYYIGE 1143
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 30.3 bits (65), Expect = 0.31
Identities = 25/105 (23%), Positives = 45/105 (42%), Gaps = 1/105 (0%)
Frame = +2
Query: 377 GKHFHPECFNCVYCG-KLFGNNPFFLEDGLPYCEADWNELFTTKCFACGFPVEAGDRWVE 553
GK +H +CF CV C KL ++ F +D + + +L C C P+ +
Sbjct: 41 GKCWHSDCFKCVNCNKKLDPSSEDFSQDDQ---KQIFCKLCVDICNGCSTPI---CEFNA 94
Query: 554 ALNNNYHSQCFNCTVCKKNLEGQSFFAKGGRPFCKTHAR*KSNKN 688
N+ + C C+ C+ + +F G+ +C R + +N
Sbjct: 95 VSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQDEEN 137
>SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 613
Score = 28.3 bits (60), Expect = 1.3
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 560 NNNYHSQCFNCTVCKKNLEGQSFFA 634
N+ Y QCF C V K+ LE + A
Sbjct: 551 NDTYFGQCFACAVVKRTLERNNITA 575
>SPBP8B7.28c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 215
Score = 28.3 bits (60), Expect = 1.3
Identities = 34/129 (26%), Positives = 54/129 (41%), Gaps = 8/129 (6%)
Frame = +2
Query: 335 KCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDGLPYCEADWNELFTTKCFA 514
K HAK LN+ GK+ + C+ C K+ G + Y + W++ FT F
Sbjct: 18 KLHAKKYRGTLNSKGKNDNDCLIMCMRCRKVKGID--------SYSKTQWSKTFT---FV 66
Query: 515 CGFPVEAGDRWV--EALNNNYHSQCFNCTVCKKNLEGQSFFAKGGR----PFCK--THAR 670
G V D V H + CT C++ +G + F+K R P C+ H++
Sbjct: 67 RGRTVSVSDPKVICRTCQPKQHDSIW-CTACQQT-KGINEFSKAQRHVLDPRCQICVHSQ 124
Query: 671 *KSNKNNLQ 697
+NL+
Sbjct: 125 RNDGDDNLE 133
>SPCC1682.08c |||RNA-binding protein Mcp2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 703
Score = 27.5 bits (58), Expect = 2.2
Identities = 19/64 (29%), Positives = 27/64 (42%), Gaps = 2/64 (3%)
Frame = -3
Query: 202 YASKSRDEWTSNVSITRCAKWHTRAGQGRLIQYSPSSFWCR*YPKSFTTCGS--SYTGTF 29
Y K+RD W++ V+++ + G L YS SS Y K + S S T
Sbjct: 268 YTVKNRDSWSNEVNLSASTNNLSTNTSGTLKPYSLSSSRSSSYSKGVNSAASLQSIWETM 327
Query: 28 KNGN 17
N N
Sbjct: 328 NNSN 331
>SPBC651.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 237
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = -3
Query: 646 PTSLGEEALSFEVLFTHRAVEALTVVVIVER 554
P +GE ++S E +FTH VE++ + + +R
Sbjct: 104 PLVVGEASVSLEDIFTHGTVESVIPLFMKKR 134
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 25.8 bits (54), Expect = 6.7
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = -3
Query: 310 LKTEFTVELSVLFDESDILQRTSACCIHADEVLGAPYASKSRDEWTSNVSITRCAKWHTR 131
L + ++ ++ E +Q S+ C D+V A YA +R NV+ CA R
Sbjct: 285 LALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAV-SKR 343
Query: 130 AGQGRLIQYSPS 95
A + IQY P+
Sbjct: 344 ACKQYSIQYFPT 355
>SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 697
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/44 (29%), Positives = 16/44 (36%)
Frame = +2
Query: 329 CDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDG 460
C H +K + LN G H C + K G N E G
Sbjct: 362 CGGAHIWLKREDLNHGGSHKINNCIGQILLAKRLGKNRIIAETG 405
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,176,517
Number of Sequences: 5004
Number of extensions: 68886
Number of successful extensions: 236
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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