BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV30985 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 306 2e-84 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 148 7e-37 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 139 3e-34 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 121 9e-29 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 2e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 78 1e-15 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 74 2e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 2e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 4e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.6 SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 7.9 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 7.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 306 bits (751), Expect = 2e-84 Identities = 141/161 (87%), Positives = 151/161 (93%) Frame = +3 Query: 3 KLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGI 182 KLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ GI Sbjct: 215 KLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGI 274 Query: 183 HETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPE 362 HE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+APPE Sbjct: 275 HEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPE 334 Query: 363 RKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 485 RKYSVWIGGSILASLSTFQQMWISK+EYDESGPGIV+RKCF Sbjct: 335 RKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 148 bits (359), Expect = 7e-37 Identities = 68/161 (42%), Positives = 103/161 (63%) Frame = +3 Query: 3 KLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGI 182 K CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ G+ Sbjct: 220 KCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGL 279 Query: 183 HETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPE 362 H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++KI A PE Sbjct: 280 HIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPE 339 Query: 363 RKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRKCF 485 R ++ W+GGSILASLSTF+++ I+ EEY ++ ++ R+ F Sbjct: 340 RMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 139 bits (337), Expect = 3e-34 Identities = 70/164 (42%), Positives = 106/164 (64%), Gaps = 12/164 (7%) Frame = +3 Query: 3 KLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGI 182 KLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ Sbjct: 215 KLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGL 274 Query: 183 HETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL--------APSTI- 335 E ++++I DVDIRK LY V+SGG++MY G+ R++KEI L P+ + Sbjct: 275 SEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLP 334 Query: 336 --KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKEEYDESG 458 K+KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 335 NFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 121 bits (292), Expect = 9e-29 Identities = 65/148 (43%), Positives = 85/148 (57%), Gaps = 11/148 (7%) Frame = +3 Query: 48 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYN 200 A AS+ K +E PDG G ERFR E LF PSF S G+HE VY Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 201 SIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYS 374 SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + + Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390 Query: 375 VWIGGSILASLSTFQQMWISKEEYDESG 458 VW GGSILASL FQ +W+SK+EYDE G Sbjct: 391 VWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 2e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = +3 Query: 177 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 356 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 357 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 482 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 78.2 bits (184), Expect = 1e-15 Identities = 54/178 (30%), Positives = 82/178 (46%), Gaps = 20/178 (11%) Frame = +3 Query: 6 LCYVALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITIGNERF 125 +CYV+ +F+++M + LE Y LPD QV+ + NERF Sbjct: 222 VCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERF 281 Query: 126 RCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQK 305 PE LF PS + + GI E V S+ +I+ L N V GG +PG R+ Sbjct: 282 MIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSS 341 Query: 306 EITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHRK 479 E+ +LAP+ ++K+ P + W S + L + I++ EY E G I+ RK Sbjct: 342 ELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 73.7 bits (173), Expect = 2e-14 Identities = 50/175 (28%), Positives = 79/175 (45%), Gaps = 19/175 (10%) Frame = +3 Query: 9 CYVALDFEQEMATAAASTS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFLGMESC 176 CYV D +E + L+ + E G TI G ERF PE F P + Sbjct: 245 CYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSDFL 304 Query: 177 G-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI------ 335 + E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I Sbjct: 305 TPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEML 364 Query: 336 --------KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPGIVHR 476 + +I+ ++ +VW GGS+LA F +K +Y+E G I R Sbjct: 365 SGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.9 bits (166), Expect = 2e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = +3 Query: 117 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 296 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 297 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 461 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = +3 Query: 231 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 401 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 402 SLSTFQQMWISKEEYDESGPGIVHRK 479 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +3 Query: 378 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 479 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = +3 Query: 303 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 413 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = -1 Query: 541 ELGRAQHHTAPGSRGRAA*KHLRWT 467 E+ R++ H+ PGS+G A +L WT Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = +2 Query: 182 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 310 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,421,752 Number of Sequences: 5004 Number of extensions: 47153 Number of successful extensions: 141 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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