Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30970
(839 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50200.1 68414.m05629 aminoacyl-tRNA synthetase family protei... 73 2e-13
At3g17810.1 68416.m02271 dihydroorotate dehydrogenase family pro... 38 0.011
At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protei... 35 0.077
At2g36990.1 68415.m04537 RNA polymerase sigma subunit SigF (sigF... 33 0.31
At3g44700.1 68416.m04807 expressed protein contains Pfam profile... 31 0.95
At3g22790.1 68416.m02873 kinase interacting family protein simil... 30 1.7
At4g31820.1 68417.m04522 phototropic-responsive NPH3 family prot... 30 2.2
At4g25990.2 68417.m03742 expressed protein 29 5.1
At4g25990.1 68417.m03741 expressed protein 29 5.1
At3g05830.1 68416.m00654 expressed protein 28 8.9
>At1g50200.1 68414.m05629 aminoacyl-tRNA synthetase family protein
contains Pfam profiles: PF01411 tRNA synthetases class
II (A), PF02272 DHHA1 domain
Length = 1003
Score = 72.9 bits (171), Expect = 2e-13
Identities = 48/163 (29%), Positives = 84/163 (51%)
Frame = +2
Query: 263 ALGYKEFKETVSKLNTSIIPGELIFKLYDTHGLQEELINRIAELNNMTTDKEGFQKLLSK 442
A G ++F++ + + + G+ F L+DT+G +L +AE + D +GF K + +
Sbjct: 440 AKGIEKFRKAGQAVQGNTLSGDDAFILWDTYGFPLDLTQLMAEERGLLVDVDGFNKAMEE 499
Query: 443 HKHRHKTAFKEQALNKTILFNDAIDRLIKNGISRTNDNFKYNYEITDSKLELGTLTTKLC 622
+ R ++A +QA ++ DA L K G+S T+D+FKY + D + EL + T
Sbjct: 500 ARERSRSAQNKQAGGAIVMDADATSTLHKAGVSATDDSFKYIW-FQDHESELKAIYT--- 555
Query: 623 AILNEDCQWIDVLDTIENRPYYLVTEDTNFYCEEGGQISDSGV 751
+++ +N LV T+FY E+GGQI D+G+
Sbjct: 556 -----GSTFLESSAASDN--VGLVLGSTSFYAEQGGQIFDTGL 591
>At3g17810.1 68416.m02271 dihydroorotate dehydrogenase family
protein / dihydroorotate oxidase family protein low
similarity to SP|Q12882 Dihydropyrimidine dehydrogenase
[NADP+] precursor (EC 1.3.1.2) (DPD) (DHPDHase)
(Dihydrouracil dehydrogenase) (Dihydrothymine
dehydrogenase) {Homo sapiens}; contains Pfam profile
PF01180: Dihydroorotate dehydrogenase
Length = 426
Score = 37.5 bits (83), Expect = 0.011
Identities = 22/83 (26%), Positives = 42/83 (50%)
Frame = +2
Query: 422 FQKLLSKHKHRHKTAFKEQALNKTILFNDAIDRLIKNGISRTNDNFKYNYEITDSKLELG 601
F++L ++ R A + NKT + + IDR+ + G+ NF + + + ++ G
Sbjct: 145 FERLKKEYPDRILIASVMEEYNKTA-WEELIDRVEQTGVDALEINFSCPHGMPERRM--G 201
Query: 602 TLTTKLCAILNEDCQWIDVLDTI 670
+ CA+L+E C WI+ T+
Sbjct: 202 AAVGQDCALLDEVCGWINAKATV 224
>At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protein
contains Pfam profiles: PF01411 tRNA synthetases class
II (A), PF02272 DHHA1 domain
Length = 978
Score = 34.7 bits (76), Expect = 0.077
Identities = 36/148 (24%), Positives = 64/148 (43%)
Frame = +2
Query: 305 NTSIIPGELIFKLYDTHGLQEELINRIAELNNMTTDKEGFQKLLSKHKHRHKTAFKEQAL 484
+T + G+ F LYDT G E+ +AE ++ D GF+ + + + + A +
Sbjct: 467 DTPYLDGKDAFLLYDTFGFPVEITAEVAEERGVSIDMNGFEVEMENQRRQSQAAHN---V 523
Query: 485 NKTILFNDAIDRLIKNGISRTNDNFKYNYEITDSKLELGTLTTKLCAILNEDCQWIDVLD 664
K + +DA + KN D Y+ ++ + +L ++N V+
Sbjct: 524 VKLTVEDDA--DMTKN----IADTEFLGYDSLSARAVVKSL------LVNGK----PVIR 567
Query: 665 TIENRPYYLVTEDTNFYCEEGGQISDSG 748
E ++ + T FY E GGQI+D G
Sbjct: 568 VSEGSEVEVLLDRTPFYAESGGQIADHG 595
>At2g36990.1 68415.m04537 RNA polymerase sigma subunit SigF (sigF) /
sigma-like factor (SIG6) identical to RNA polymerase
sigma subunit SigF [Arabidopsis thaliana] GI:7209640;
contains Pfam profiles PF04545: Sigma-70, region 4,
PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2;
identical to cDNA partial mRNA for putative sigma-like
transcription factor (sig6 gene) GI:6273429
Length = 547
Score = 32.7 bits (71), Expect = 0.31
Identities = 25/99 (25%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
Frame = +2
Query: 197 WKDLVTRYPEVESLNDVETPGFALGYKEFKETVSKLNTSIIPGE-LIFKL-YDTHGLQEE 370
W D T + E+ + +ETP ++G + + V L + P E I KL + G ++
Sbjct: 444 WSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQR 503
Query: 371 LINRIAELNNMTTDKEGFQKLLSKHKHRHKTAFKEQALN 487
++ I E+ ++ KE ++L S+ +R K L+
Sbjct: 504 SLSEIGEIYGLS--KERVRQLESRALYRLKQNMNSHGLH 540
>At3g44700.1 68416.m04807 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function; expression
supported by MPSS
Length = 368
Score = 31.1 bits (67), Expect = 0.95
Identities = 20/86 (23%), Positives = 38/86 (44%), Gaps = 2/86 (2%)
Frame = +2
Query: 380 RIAELNNMTTDKEGFQKLLSKHKHRHKTAFKEQALNKTILFNDAIDRL--IKNGISRTND 553
R+ + KE Q ++ +HKHR F ++A K + D I+ + ++ I +
Sbjct: 69 RVLSIGPYHHGKEHLQ-MIQEHKHRFLGIFMDEAQKKGVDMKDLIEAVSELEEDIRESYS 127
Query: 554 NFKYNYEITDSKLELGTLTTKLCAIL 631
YN +++ K + + C IL
Sbjct: 128 ESLYNGDVSGRKKLIDMMVLDCCFIL 153
>At3g22790.1 68416.m02873 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1694
Score = 30.3 bits (65), Expect = 1.7
Identities = 30/107 (28%), Positives = 51/107 (47%), Gaps = 2/107 (1%)
Frame = +2
Query: 2 IRHEAFKICSDVFNNPHLLSNLYDEVAATLS--STYPELVSNKSSAKLIIEHEAQAYAKM 175
+ + A K ++V N S L+ E A L+ + E++SN E AQ ++
Sbjct: 263 LTNRATKAETEVENLKQAHSRLHSEKEAGLAEYNRCLEMISNLEKKVRDAEENAQNFSNQ 322
Query: 176 RAGLAKKWKDLVTRYPEVESLNDVETPGFALGYKEFKETVSKLNTSI 316
A + K L R+ E+ +N+V+ G L Y++ ET+SKL +
Sbjct: 323 SAKAEDEIKAL--RH-ELVKVNEVKD-GLRLRYQQCLETISKLEREV 365
>At4g31820.1 68417.m04522 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 571
Score = 29.9 bits (64), Expect = 2.2
Identities = 18/77 (23%), Positives = 34/77 (44%)
Frame = +2
Query: 8 HEAFKICSDVFNNPHLLSNLYDEVAATLSSTYPELVSNKSSAKLIIEHEAQAYAKMRAGL 187
++AF+IC+ + N Y+ A ++ Y E+ + LI + E + +G+
Sbjct: 78 YKAFEICAKFCYGMTVTLNAYNITAVRCAAEYLEMTEDADRGNLIYKIE----VFLNSGI 133
Query: 188 AKKWKDLVTRYPEVESL 238
+ WKD + SL
Sbjct: 134 FRSWKDSIIVLQTTRSL 150
>At4g25990.2 68417.m03742 expressed protein
Length = 409
Score = 28.7 bits (61), Expect = 5.1
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 68 YDEVAATLSSTYPELVSNKSS 130
YDE AA LS+ YP++ S+K +
Sbjct: 54 YDEAAALLSTAYPKIFSSKKA 74
>At4g25990.1 68417.m03741 expressed protein
Length = 394
Score = 28.7 bits (61), Expect = 5.1
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 68 YDEVAATLSSTYPELVSNKSS 130
YDE AA LS+ YP++ S+K +
Sbjct: 54 YDEAAALLSTAYPKIFSSKKA 74
>At3g05830.1 68416.m00654 expressed protein
Length = 336
Score = 27.9 bits (59), Expect = 8.9
Identities = 19/72 (26%), Positives = 32/72 (44%)
Frame = +2
Query: 275 KEFKETVSKLNTSIIPGELIFKLYDTHGLQEELINRIAELNNMTTDKEGFQKLLSKHKHR 454
+E E ++K + EL L D ++ E +NR+ E+ + K + L + +
Sbjct: 179 REVTEAIAKAGIGGMDSELQKLLEDVSPMKFERMNRLVEVKDEEITKLKDEIRLMSGQWK 238
Query: 455 HKTAFKEQALNK 490
HKT E L K
Sbjct: 239 HKTKELESQLEK 250
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,339,016
Number of Sequences: 28952
Number of extensions: 320325
Number of successful extensions: 984
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 984
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1941125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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