Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV30962
(701 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 71 2e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 57 2e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 32 0.091
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.5
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 28 1.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.6
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.0
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 7.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.9 bits (166), Expect = 2e-13
Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
Frame = +1
Query: 64 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 237
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 238 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 414
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 415 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 507
EVA++ ++ D DG+I Y F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 2e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +1
Query: 106 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 285
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 286 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 465
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 466 DDGMIPYAAFLKKVMA 513
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 57.2 bits (132), Expect = 2e-09
Identities = 37/146 (25%), Positives = 71/146 (48%)
Frame = +1
Query: 76 SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 255
SK + AF +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 256 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 435
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 436 VTKDCMDPEDDDGMIPYAAFLKKVMA 513
+ ++ DP + G Y F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.9 bits (69), Expect = 0.091
Identities = 33/161 (20%), Positives = 63/161 (39%), Gaps = 1/161 (0%)
Frame = +1
Query: 31 RPGGNRQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 210
R GG ++++ + D+ A F ++D + ID L +RAL N + +
Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76
Query: 211 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 387
F+ + ++ ++D E+ +L+D +E G + L
Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134
Query: 388 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 510
L E +DD E+ + ++ D DG I F+ +M
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.5
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 666 APPEELSPPRALPAPVPQSRASVF*GPSHRT 574
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 27.9 bits (59), Expect = 1.5
Identities = 22/97 (22%), Positives = 44/97 (45%), Gaps = 2/97 (2%)
Frame = +2
Query: 38 EGTD-KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSR 211
E TD ++HT T A+ KG P++ KAK+ + ++ +S Q P++
Sbjct: 38 ERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTK 97
Query: 212 NSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 322
+S+ + + + SK S+ ++ + +IS
Sbjct: 98 HSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 64 MSDLSKNDVER-ASFAFSIYDFEGKGKID 147
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.0
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 400 LGEKLDDSEVAEVTKDCMDPED 465
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 7.9
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +2
Query: 83 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 256
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 257 KSSFPSTAKQRKTKT 301
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,585,562
Number of Sequences: 5004
Number of extensions: 48370
Number of successful extensions: 149
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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